Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13410 and BWI76_RS13415 are separated by 1 nucleotides BWI76_RS13415 and BWI76_RS13420 overlap by 11 nucleotides BWI76_RS13420 and BWI76_RS13425 are separated by 22 nucleotides
BWI76_RS13410: BWI76_RS13410 - polyamine ABC transporter ATP-binding protein, at 2,797,268 to 2,798,278
_RS13410
BWI76_RS13415: BWI76_RS13415 - spermidine/putrescine ABC transporter permease, at 2,798,280 to 2,799,209
_RS13415
BWI76_RS13420: BWI76_RS13420 - ABC transporter permease, at 2,799,199 to 2,800,005
_RS13420
BWI76_RS13425: BWI76_RS13425 - 1-pyrroline dehydrogenase, at 2,800,028 to 2,801,455
_RS13425
Position (kb)
2799
2800
2801 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2798.327 kb on + strand at 2798.475 kb on - strand, within BWI76_RS13415 at 2798.596 kb on + strand, within BWI76_RS13415 at 2798.604 kb on - strand, within BWI76_RS13415 at 2798.693 kb on - strand, within BWI76_RS13415 at 2799.088 kb on + strand, within BWI76_RS13415 at 2799.296 kb on - strand, within BWI76_RS13420 at 2799.351 kb on + strand, within BWI76_RS13420 at 2800.018 kb on + strand at 2800.059 kb on - strand at 2800.147 kb on + strand at 2800.309 kb on - strand, within BWI76_RS13425 at 2800.758 kb on + strand, within BWI76_RS13425 at 2800.823 kb on - strand, within BWI76_RS13425 at 2800.846 kb on + strand, within BWI76_RS13425 at 2800.890 kb on - strand, within BWI76_RS13425
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,798,327 + +1.3 2,798,475 - BWI76_RS13415 0.21 +0.2 2,798,596 + BWI76_RS13415 0.34 -2.2 2,798,604 - BWI76_RS13415 0.35 +0.6 2,798,693 - BWI76_RS13415 0.44 +0.5 2,799,088 + BWI76_RS13415 0.87 -3.8 2,799,296 - BWI76_RS13420 0.12 -3.9 2,799,351 + BWI76_RS13420 0.19 +1.0 2,800,018 + -1.3 2,800,059 - +0.5 2,800,147 + -1.6 2,800,309 - BWI76_RS13425 0.20 -1.0 2,800,758 + BWI76_RS13425 0.51 -0.0 2,800,823 - BWI76_RS13425 0.56 +1.5 2,800,846 + BWI76_RS13425 0.57 +0.9 2,800,890 - BWI76_RS13425 0.60 -0.1
Or see this region's nucleotide sequence