Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09600 and BWI76_RS09605 are separated by 68 nucleotides BWI76_RS09605 and BWI76_RS09610 are separated by 324 nucleotides BWI76_RS09610 and BWI76_RS09615 are separated by 30 nucleotides BWI76_RS09615 and BWI76_RS09620 are separated by 138 nucleotides BWI76_RS09620 and BWI76_RS09625 overlap by 73 nucleotides BWI76_RS09625 and BWI76_RS09630 are separated by 0 nucleotides
BWI76_RS09600: BWI76_RS09600 - macrolide ABC transporter permease/ATP-binding protein MacB, at 2,008,593 to 2,010,539
_RS09600
BWI76_RS09605: BWI76_RS09605 - cold-shock protein, at 2,010,608 to 2,010,838
_RS09605
BWI76_RS09610: BWI76_RS09610 - ATP-dependent Clp protease adaptor ClpS, at 2,011,163 to 2,011,480
_RS09610
BWI76_RS09615: BWI76_RS09615 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,011,511 to 2,013,793
_RS09615
BWI76_RS09620: BWI76_RS09620 - translation initiation factor IF-1, at 2,013,932 to 2,014,150
_RS09620
BWI76_RS09625: BWI76_RS09625 - hypothetical protein, at 2,014,078 to 2,014,429
_RS09625
BWI76_RS09630: BWI76_RS09630 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,014,430 to 2,015,134
_RS09630
Position (kb)
2011
2012
2013
2014 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2010.584 kb on + strand at 2010.592 kb on - strand at 2010.832 kb on + strand at 2011.146 kb on - strand at 2011.165 kb on + strand at 2011.267 kb on - strand, within BWI76_RS09610 at 2011.337 kb on - strand, within BWI76_RS09610 at 2011.456 kb on + strand at 2011.579 kb on + strand at 2011.612 kb on + strand at 2012.273 kb on + strand, within BWI76_RS09615 at 2012.492 kb on + strand, within BWI76_RS09615 at 2013.343 kb on + strand, within BWI76_RS09615 at 2013.483 kb on - strand, within BWI76_RS09615 at 2014.300 kb on + strand, within BWI76_RS09625
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,010,584 + +0.6 2,010,592 - -0.3 2,010,832 + +1.0 2,011,146 - -0.5 2,011,165 + -0.0 2,011,267 - BWI76_RS09610 0.33 +1.1 2,011,337 - BWI76_RS09610 0.55 -1.0 2,011,456 + +0.2 2,011,579 + -0.6 2,011,612 + -0.5 2,012,273 + BWI76_RS09615 0.33 -3.5 2,012,492 + BWI76_RS09615 0.43 -3.3 2,013,343 + BWI76_RS09615 0.80 +1.1 2,013,483 - BWI76_RS09615 0.86 -0.5 2,014,300 + BWI76_RS09625 0.63 -1.7
Or see this region's nucleotide sequence