Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06550 and BWI76_RS06555 are separated by 110 nucleotides BWI76_RS06555 and BWI76_RS06560 are separated by 201 nucleotides BWI76_RS06560 and BWI76_RS06565 are separated by 66 nucleotides
BWI76_RS06550: BWI76_RS06550 - competence protein ComEA, at 1,377,773 to 1,378,138
_RS06550
BWI76_RS06555: BWI76_RS06555 - thioesterase superfamily protein, at 1,378,249 to 1,378,650
_RS06555
BWI76_RS06560: BWI76_RS06560 - 7-cyano-7-deazaguanine synthase QueC, at 1,378,852 to 1,379,547
_RS06560
BWI76_RS06565: BWI76_RS06565 - putative periplasmic solute-binding protein, at 1,379,614 to 1,381,314
_RS06565
Position (kb)
1378
1379
1380 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1377.918 kb on + strand, within BWI76_RS06550 at 1378.025 kb on + strand, within BWI76_RS06550 at 1378.079 kb on - strand, within BWI76_RS06550 at 1378.745 kb on + strand at 1378.987 kb on + strand, within BWI76_RS06560 at 1378.992 kb on + strand, within BWI76_RS06560 at 1379.119 kb on + strand, within BWI76_RS06560 at 1379.156 kb on - strand, within BWI76_RS06560 at 1379.766 kb on - strand at 1379.929 kb on - strand, within BWI76_RS06565 at 1380.101 kb on - strand, within BWI76_RS06565 at 1380.215 kb on - strand, within BWI76_RS06565 at 1380.284 kb on - strand, within BWI76_RS06565 at 1380.416 kb on - strand, within BWI76_RS06565
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,377,918 + BWI76_RS06550 0.40 +0.9 1,378,025 + BWI76_RS06550 0.69 +0.1 1,378,079 - BWI76_RS06550 0.84 +0.1 1,378,745 + +1.1 1,378,987 + BWI76_RS06560 0.19 +1.3 1,378,992 + BWI76_RS06560 0.20 +1.5 1,379,119 + BWI76_RS06560 0.38 -2.8 1,379,156 - BWI76_RS06560 0.44 +0.2 1,379,766 - -0.1 1,379,929 - BWI76_RS06565 0.19 +1.0 1,380,101 - BWI76_RS06565 0.29 +1.1 1,380,215 - BWI76_RS06565 0.35 +1.4 1,380,284 - BWI76_RS06565 0.39 -2.2 1,380,416 - BWI76_RS06565 0.47 -3.3
Or see this region's nucleotide sequence