Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04885 and BWI76_RS04890 are separated by 207 nucleotides BWI76_RS04890 and BWI76_RS04895 are separated by 162 nucleotides BWI76_RS04895 and BWI76_RS04900 are separated by 50 nucleotides
BWI76_RS04885: BWI76_RS04885 - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,022,684 to 1,024,573
_RS04885
BWI76_RS04890: BWI76_RS04890 - dihydrolipoyl dehydrogenase, at 1,024,781 to 1,026,205
_RS04890
BWI76_RS04895: BWI76_RS04895 - hypothetical protein, at 1,026,368 to 1,026,652
_RS04895
BWI76_RS04900: BWI76_RS04900 - DUF2950 domain-containing protein, at 1,026,703 to 1,027,497
_RS04900
Position (kb) 1024
1025
1026
1027 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1026.265 kb on + strand at 1026.273 kb on - strand at 1026.273 kb on - strand at 1026.384 kb on - strand at 1026.400 kb on - strand, within BWI76_RS04895 at 1026.416 kb on + strand, within BWI76_RS04895 at 1026.424 kb on - strand, within BWI76_RS04895 at 1026.944 kb on - strand, within BWI76_RS04900
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,026,265 + -4.4 1,026,273 - -1.6 1,026,273 - +1.3 1,026,384 - +2.2 1,026,400 - BWI76_RS04895 0.11 -2.7 1,026,416 + BWI76_RS04895 0.17 -0.8 1,026,424 - BWI76_RS04895 0.20 -1.3 1,026,944 - BWI76_RS04900 0.30 +0.1
Or see this region's nucleotide sequence