Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03730 and BWI76_RS03735 are separated by 38 nucleotides BWI76_RS03735 and BWI76_RS03740 overlap by 4 nucleotides BWI76_RS03740 and BWI76_RS03745 are separated by 8 nucleotides
BWI76_RS03730: BWI76_RS03730 - amidohydrolase, at 773,398 to 774,501
_RS03730
BWI76_RS03735: BWI76_RS03735 - hypothetical protein, at 774,540 to 774,794
_RS03735
BWI76_RS03740: BWI76_RS03740 - hypothetical protein, at 774,791 to 775,558
_RS03740
BWI76_RS03745: BWI76_RS03745 - putative 2-hydroxyglutaryl-CoA dehydratase, at 775,567 to 776,718
_RS03745
Position (kb) 774
775
776 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 773.842 kb on + strand, within BWI76_RS03730 at 773.844 kb on + strand, within BWI76_RS03730 at 773.850 kb on - strand, within BWI76_RS03730 at 773.888 kb on - strand, within BWI76_RS03730 at 773.934 kb on - strand, within BWI76_RS03730 at 774.129 kb on + strand, within BWI76_RS03730 at 774.166 kb on + strand, within BWI76_RS03730 at 774.183 kb on + strand, within BWI76_RS03730 at 774.249 kb on + strand, within BWI76_RS03730 at 774.355 kb on - strand, within BWI76_RS03730 at 774.366 kb on + strand, within BWI76_RS03730 at 774.366 kb on + strand, within BWI76_RS03730 at 774.490 kb on + strand at 774.635 kb on - strand at 774.808 kb on + strand at 774.873 kb on + strand, within BWI76_RS03740 at 774.889 kb on + strand, within BWI76_RS03740 at 774.897 kb on - strand, within BWI76_RS03740 at 774.897 kb on - strand, within BWI76_RS03740 at 775.074 kb on + strand, within BWI76_RS03740 at 775.279 kb on - strand, within BWI76_RS03740 at 775.402 kb on + strand, within BWI76_RS03740 at 775.432 kb on + strand, within BWI76_RS03740 at 775.467 kb on + strand, within BWI76_RS03740 at 775.507 kb on + strand at 775.598 kb on + strand at 775.756 kb on - strand, within BWI76_RS03745 at 775.783 kb on - strand, within BWI76_RS03745 at 775.866 kb on + strand, within BWI76_RS03745 at 776.359 kb on - strand, within BWI76_RS03745 at 776.431 kb on - strand, within BWI76_RS03745 at 776.492 kb on - strand, within BWI76_RS03745
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 773,842 + BWI76_RS03730 0.40 -0.5 773,844 + BWI76_RS03730 0.40 +1.8 773,850 - BWI76_RS03730 0.41 +1.3 773,888 - BWI76_RS03730 0.44 -0.3 773,934 - BWI76_RS03730 0.49 +0.6 774,129 + BWI76_RS03730 0.66 +2.4 774,166 + BWI76_RS03730 0.70 +1.5 774,183 + BWI76_RS03730 0.71 -0.5 774,249 + BWI76_RS03730 0.77 -1.3 774,355 - BWI76_RS03730 0.87 -3.7 774,366 + BWI76_RS03730 0.88 +0.8 774,366 + BWI76_RS03730 0.88 -1.5 774,490 + +1.1 774,635 - -2.2 774,808 + +1.3 774,873 + BWI76_RS03740 0.11 +0.7 774,889 + BWI76_RS03740 0.13 -1.3 774,897 - BWI76_RS03740 0.14 +1.4 774,897 - BWI76_RS03740 0.14 +0.8 775,074 + BWI76_RS03740 0.37 +0.8 775,279 - BWI76_RS03740 0.64 +0.5 775,402 + BWI76_RS03740 0.80 +1.2 775,432 + BWI76_RS03740 0.83 +1.1 775,467 + BWI76_RS03740 0.88 -1.1 775,507 + -1.3 775,598 + -4.2 775,756 - BWI76_RS03745 0.16 -1.7 775,783 - BWI76_RS03745 0.19 -0.1 775,866 + BWI76_RS03745 0.26 +1.3 776,359 - BWI76_RS03745 0.69 -0.3 776,431 - BWI76_RS03745 0.75 -0.7 776,492 - BWI76_RS03745 0.80 +1.5
Or see this region's nucleotide sequence