Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03530 and BWI76_RS03535 are separated by 138 nucleotides BWI76_RS03535 and BWI76_RS03540 are separated by 123 nucleotides BWI76_RS03540 and BWI76_RS03545 are separated by 241 nucleotides BWI76_RS03545 and BWI76_RS03550 are separated by 17 nucleotides
BWI76_RS03530: BWI76_RS03530 - tryptophan permease, at 732,670 to 733,926
_RS03530
BWI76_RS03535: BWI76_RS03535 - Tryptophanase (EC 4.1.99.1) <small>(from data)</small>, at 734,065 to 735,453
_RS03535
BWI76_RS03540: BWI76_RS03540 - tryptophanase leader peptide, at 735,577 to 735,672
_RS03540
BWI76_RS03545: BWI76_RS03545 - QacE family quaternary ammonium compound efflux SMR transporter, at 735,914 to 736,243
_RS03545
BWI76_RS03550: BWI76_RS03550 - QacE family quaternary ammonium compound efflux SMR transporter, at 736,261 to 736,680
_RS03550
Position (kb) 734
735
736 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 733.067 kb on - strand, within BWI76_RS03530 at 733.087 kb on - strand, within BWI76_RS03530 at 733.099 kb on + strand, within BWI76_RS03530 at 733.208 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.339 kb on + strand, within BWI76_RS03530 at 733.359 kb on + strand, within BWI76_RS03530 at 733.404 kb on - strand, within BWI76_RS03530 at 733.444 kb on + strand, within BWI76_RS03530 at 733.491 kb on + strand, within BWI76_RS03530 at 733.491 kb on + strand, within BWI76_RS03530 at 733.499 kb on - strand, within BWI76_RS03530 at 733.516 kb on + strand, within BWI76_RS03530 at 733.524 kb on - strand, within BWI76_RS03530 at 733.534 kb on + strand, within BWI76_RS03530 at 733.538 kb on + strand, within BWI76_RS03530 at 733.542 kb on - strand, within BWI76_RS03530 at 733.575 kb on + strand, within BWI76_RS03530 at 733.583 kb on - strand, within BWI76_RS03530 at 733.614 kb on - strand, within BWI76_RS03530 at 733.615 kb on + strand, within BWI76_RS03530 at 733.619 kb on - strand, within BWI76_RS03530 at 733.675 kb on - strand, within BWI76_RS03530 at 733.686 kb on + strand, within BWI76_RS03530 at 733.694 kb on - strand, within BWI76_RS03530 at 733.694 kb on - strand, within BWI76_RS03530 at 733.731 kb on + strand, within BWI76_RS03530 at 733.732 kb on + strand, within BWI76_RS03530 at 733.732 kb on + strand, within BWI76_RS03530 at 733.739 kb on - strand, within BWI76_RS03530 at 733.742 kb on + strand, within BWI76_RS03530 at 733.751 kb on + strand, within BWI76_RS03530 at 733.752 kb on - strand, within BWI76_RS03530 at 733.763 kb on + strand, within BWI76_RS03530 at 733.769 kb on - strand, within BWI76_RS03530 at 733.772 kb on + strand, within BWI76_RS03530 at 733.774 kb on + strand, within BWI76_RS03530 at 733.884 kb on + strand at 733.886 kb on - strand at 733.892 kb on - strand at 733.892 kb on - strand at 733.922 kb on + strand at 733.922 kb on + strand at 733.922 kb on + strand at 733.923 kb on + strand at 733.930 kb on - strand at 733.930 kb on - strand at 733.952 kb on - strand at 733.954 kb on - strand at 733.955 kb on - strand at 733.977 kb on + strand at 733.979 kb on - strand at 733.992 kb on + strand at 733.992 kb on + strand at 734.000 kb on - strand at 734.000 kb on - strand at 734.000 kb on - strand at 734.006 kb on + strand at 734.013 kb on - strand at 734.058 kb on - strand at 734.083 kb on - strand at 734.084 kb on - strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.098 kb on + strand at 734.106 kb on - strand at 734.106 kb on - strand at 734.106 kb on - strand at 734.106 kb on - strand at 734.111 kb on - strand at 734.111 kb on - strand at 734.113 kb on - strand at 734.113 kb on - strand at 734.120 kb on - strand at 734.120 kb on - strand at 734.127 kb on - strand at 734.132 kb on + strand at 734.140 kb on - strand at 734.140 kb on - strand at 734.154 kb on + strand at 734.154 kb on + strand at 734.162 kb on - strand at 734.162 kb on - strand at 734.162 kb on - strand at 734.162 kb on - strand at 734.200 kb on + strand at 734.230 kb on + strand, within BWI76_RS03535 at 734.361 kb on + strand, within BWI76_RS03535 at 734.449 kb on + strand, within BWI76_RS03535 at 734.503 kb on - strand, within BWI76_RS03535 at 734.557 kb on - strand, within BWI76_RS03535 at 734.609 kb on + strand, within BWI76_RS03535 at 734.639 kb on - strand, within BWI76_RS03535 at 734.644 kb on - strand, within BWI76_RS03535 at 734.686 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.706 kb on + strand, within BWI76_RS03535 at 734.744 kb on + strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.763 kb on + strand, within BWI76_RS03535 at 734.836 kb on + strand, within BWI76_RS03535 at 734.842 kb on - strand, within BWI76_RS03535 at 734.844 kb on - strand, within BWI76_RS03535 at 734.885 kb on + strand, within BWI76_RS03535 at 734.887 kb on + strand, within BWI76_RS03535 at 734.893 kb on + strand, within BWI76_RS03535 at 734.940 kb on - strand, within BWI76_RS03535 at 734.942 kb on - strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 735.009 kb on - strand, within BWI76_RS03535 at 735.047 kb on + strand, within BWI76_RS03535 at 735.144 kb on + strand, within BWI76_RS03535 at 735.157 kb on - strand, within BWI76_RS03535 at 735.159 kb on + strand, within BWI76_RS03535 at 735.174 kb on - strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.183 kb on - strand, within BWI76_RS03535 at 735.232 kb on - strand, within BWI76_RS03535 at 735.238 kb on + strand, within BWI76_RS03535 at 735.266 kb on - strand, within BWI76_RS03535 at 735.284 kb on - strand, within BWI76_RS03535 at 735.294 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.315 kb on + strand at 735.320 kb on + strand at 735.320 kb on + strand at 735.327 kb on - strand at 735.362 kb on + strand at 735.362 kb on + strand at 735.366 kb on + strand at 735.374 kb on + strand at 735.375 kb on - strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.409 kb on + strand at 735.433 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.468 kb on + strand at 735.481 kb on + strand at 735.596 kb on - strand, within BWI76_RS03540 at 735.611 kb on + strand, within BWI76_RS03540 at 735.612 kb on + strand, within BWI76_RS03540 at 735.614 kb on - strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.639 kb on + strand, within BWI76_RS03540 at 735.642 kb on - strand, within BWI76_RS03540 at 735.674 kb on + strand at 735.682 kb on - strand at 735.688 kb on + strand at 735.697 kb on - strand at 735.770 kb on + strand at 735.803 kb on + strand at 735.806 kb on + strand at 735.827 kb on - strand at 735.880 kb on + strand at 735.912 kb on + strand at 735.912 kb on + strand at 735.913 kb on + strand at 735.914 kb on - strand at 735.920 kb on - strand at 735.964 kb on - strand, within BWI76_RS03545 at 735.964 kb on - strand, within BWI76_RS03545 at 735.974 kb on - strand, within BWI76_RS03545 at 736.036 kb on + strand, within BWI76_RS03545 at 736.038 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.094 kb on + strand, within BWI76_RS03545 at 736.102 kb on - strand, within BWI76_RS03545 at 736.104 kb on + strand, within BWI76_RS03545 at 736.112 kb on - strand, within BWI76_RS03545 at 736.149 kb on + strand, within BWI76_RS03545
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 733,067 - BWI76_RS03530 0.32 -0.5 733,087 - BWI76_RS03530 0.33 +2.3 733,099 + BWI76_RS03530 0.34 +0.9 733,208 + BWI76_RS03530 0.43 +1.2 733,339 + BWI76_RS03530 0.53 +2.6 733,339 + BWI76_RS03530 0.53 +1.2 733,359 + BWI76_RS03530 0.55 -0.3 733,404 - BWI76_RS03530 0.58 -2.9 733,444 + BWI76_RS03530 0.62 -0.8 733,491 + BWI76_RS03530 0.65 +1.4 733,491 + BWI76_RS03530 0.65 -0.8 733,499 - BWI76_RS03530 0.66 +0.2 733,516 + BWI76_RS03530 0.67 -0.8 733,524 - BWI76_RS03530 0.68 +1.2 733,534 + BWI76_RS03530 0.69 +0.5 733,538 + BWI76_RS03530 0.69 -2.6 733,542 - BWI76_RS03530 0.69 -0.3 733,575 + BWI76_RS03530 0.72 +0.5 733,583 - BWI76_RS03530 0.73 +0.6 733,614 - BWI76_RS03530 0.75 +0.8 733,615 + BWI76_RS03530 0.75 +0.8 733,619 - BWI76_RS03530 0.75 -2.8 733,675 - BWI76_RS03530 0.80 +1.7 733,686 + BWI76_RS03530 0.81 -3.8 733,694 - BWI76_RS03530 0.81 -1.7 733,694 - BWI76_RS03530 0.81 -0.8 733,731 + BWI76_RS03530 0.84 +2.6 733,732 + BWI76_RS03530 0.84 -1.1 733,732 + BWI76_RS03530 0.84 +0.4 733,739 - BWI76_RS03530 0.85 -0.1 733,742 + BWI76_RS03530 0.85 -0.2 733,751 + BWI76_RS03530 0.86 +1.2 733,752 - BWI76_RS03530 0.86 +0.2 733,763 + BWI76_RS03530 0.87 -1.3 733,769 - BWI76_RS03530 0.87 -2.1 733,772 + BWI76_RS03530 0.88 -0.0 733,774 + BWI76_RS03530 0.88 +0.3 733,884 + -1.7 733,886 - +0.7 733,892 - +0.2 733,892 - -3.3 733,922 + -2.1 733,922 + -2.8 733,922 + -1.8 733,923 + -0.8 733,930 - +1.7 733,930 - -0.0 733,952 - +0.2 733,954 - +1.6 733,955 - -1.9 733,977 + -1.3 733,979 - -0.4 733,992 + -0.6 733,992 + +2.1 734,000 - -0.5 734,000 - +0.5 734,000 - -0.2 734,006 + +0.9 734,013 - +0.4 734,058 - +0.7 734,083 - +0.2 734,084 - -1.0 734,098 + -0.1 734,098 + +0.8 734,098 + +1.2 734,098 + +1.0 734,098 + +0.2 734,098 + -0.4 734,098 + +0.1 734,098 + +0.4 734,106 - -1.2 734,106 - +1.8 734,106 - +0.6 734,106 - +0.2 734,111 - -0.8 734,111 - +0.7 734,113 - -1.5 734,113 - -0.2 734,120 - +1.4 734,120 - +1.3 734,127 - -1.4 734,132 + -2.5 734,140 - +0.8 734,140 - -0.4 734,154 + -1.2 734,154 + +0.5 734,162 - +0.3 734,162 - +2.4 734,162 - -3.2 734,162 - -2.1 734,200 + +0.8 734,230 + BWI76_RS03535 0.12 -2.7 734,361 + BWI76_RS03535 0.21 +1.1 734,449 + BWI76_RS03535 0.28 +0.7 734,503 - BWI76_RS03535 0.32 +1.0 734,557 - BWI76_RS03535 0.35 +1.6 734,609 + BWI76_RS03535 0.39 -1.4 734,639 - BWI76_RS03535 0.41 +1.0 734,644 - BWI76_RS03535 0.42 -1.1 734,686 + BWI76_RS03535 0.45 +1.0 734,691 + BWI76_RS03535 0.45 +1.2 734,691 + BWI76_RS03535 0.45 +1.7 734,691 + BWI76_RS03535 0.45 +0.2 734,691 + BWI76_RS03535 0.45 -1.5 734,691 + BWI76_RS03535 0.45 +0.5 734,691 + BWI76_RS03535 0.45 +1.5 734,694 - BWI76_RS03535 0.45 +0.3 734,694 - BWI76_RS03535 0.45 -1.5 734,699 - BWI76_RS03535 0.46 +0.3 734,699 - BWI76_RS03535 0.46 +0.1 734,699 - BWI76_RS03535 0.46 -1.5 734,706 + BWI76_RS03535 0.46 -0.7 734,744 + BWI76_RS03535 0.49 -2.6 734,752 - BWI76_RS03535 0.49 +0.3 734,752 - BWI76_RS03535 0.49 +1.0 734,763 + BWI76_RS03535 0.50 +0.5 734,836 + BWI76_RS03535 0.56 +1.6 734,842 - BWI76_RS03535 0.56 -2.1 734,844 - BWI76_RS03535 0.56 +0.3 734,885 + BWI76_RS03535 0.59 +0.5 734,887 + BWI76_RS03535 0.59 -0.8 734,893 + BWI76_RS03535 0.60 -0.5 734,940 - BWI76_RS03535 0.63 -1.8 734,942 - BWI76_RS03535 0.63 -3.3 734,989 + BWI76_RS03535 0.67 -0.1 734,989 + BWI76_RS03535 0.67 -0.2 735,009 - BWI76_RS03535 0.68 +2.5 735,047 + BWI76_RS03535 0.71 +0.2 735,144 + BWI76_RS03535 0.78 +1.9 735,157 - BWI76_RS03535 0.79 +1.5 735,159 + BWI76_RS03535 0.79 +1.1 735,174 - BWI76_RS03535 0.80 +0.5 735,176 + BWI76_RS03535 0.80 -1.4 735,176 + BWI76_RS03535 0.80 +0.7 735,176 + BWI76_RS03535 0.80 +0.2 735,183 - BWI76_RS03535 0.80 +0.1 735,232 - BWI76_RS03535 0.84 -1.8 735,238 + BWI76_RS03535 0.84 +1.3 735,266 - BWI76_RS03535 0.86 -1.1 735,284 - BWI76_RS03535 0.88 -1.6 735,294 - BWI76_RS03535 0.88 +0.2 735,299 - BWI76_RS03535 0.89 +0.4 735,299 - BWI76_RS03535 0.89 +1.2 735,315 + +1.2 735,320 + +0.3 735,320 + -2.4 735,327 - -2.1 735,362 + +0.0 735,362 + +0.2 735,366 + -0.8 735,374 + -3.7 735,375 - -0.8 735,387 + +1.2 735,387 + -2.1 735,387 + -0.8 735,387 + +0.1 735,387 + +0.0 735,387 + -1.6 735,387 + +1.2 735,387 + -1.8 735,387 + -0.4 735,387 + +0.2 735,387 + +0.4 735,387 + -1.8 735,387 + +0.2 735,387 + +0.2 735,387 + -0.4 735,387 + +1.2 735,387 + +1.1 735,387 + +0.1 735,387 + -2.2 735,395 - -1.4 735,395 - +0.7 735,395 - +1.3 735,395 - -0.4 735,395 - +2.2 735,395 - +1.4 735,395 - +1.2 735,395 - +0.3 735,395 - -2.9 735,409 + -1.4 735,433 + +0.3 735,435 + -1.6 735,435 + -1.6 735,435 + +0.3 735,435 + -2.1 735,435 + -1.6 735,435 + -0.6 735,443 - -0.4 735,443 - -2.6 735,443 - -0.4 735,443 - -1.1 735,443 - +0.2 735,443 - +1.6 735,468 + +1.9 735,481 + +1.2 735,596 - BWI76_RS03540 0.20 +1.1 735,611 + BWI76_RS03540 0.35 -0.4 735,612 + BWI76_RS03540 0.36 +0.4 735,614 - BWI76_RS03540 0.39 -2.5 735,634 + BWI76_RS03540 0.59 +0.3 735,634 + BWI76_RS03540 0.59 +0.2 735,634 + BWI76_RS03540 0.59 -2.4 735,634 + BWI76_RS03540 0.59 -1.1 735,639 + BWI76_RS03540 0.65 -2.2 735,642 - BWI76_RS03540 0.68 -3.2 735,674 + +2.3 735,682 - -0.4 735,688 + -2.7 735,697 - -2.2 735,770 + +2.9 735,803 + -2.7 735,806 + -3.8 735,827 - -2.9 735,880 + +0.6 735,912 + +0.3 735,912 + +0.8 735,913 + -3.3 735,914 - -1.1 735,920 - +0.5 735,964 - BWI76_RS03545 0.15 +0.2 735,964 - BWI76_RS03545 0.15 -1.4 735,974 - BWI76_RS03545 0.18 +1.3 736,036 + BWI76_RS03545 0.37 -1.4 736,038 + BWI76_RS03545 0.38 +1.2 736,075 + BWI76_RS03545 0.49 -0.7 736,075 + BWI76_RS03545 0.49 -1.6 736,094 + BWI76_RS03545 0.55 -1.1 736,102 - BWI76_RS03545 0.57 +2.0 736,104 + BWI76_RS03545 0.58 -0.4 736,112 - BWI76_RS03545 0.60 -0.3 736,149 + BWI76_RS03545 0.71 -0.8
Or see this region's nucleotide sequence