Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02315 and BWI76_RS02320 overlap by 25 nucleotides BWI76_RS02320 and BWI76_RS02325 are separated by 94 nucleotides BWI76_RS02325 and BWI76_RS02330 are separated by 118 nucleotides
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
BWI76_RS02320: BWI76_RS02320 - divalent ion tolerance protein CutA, at 477,576 to 477,899
_RS02320
BWI76_RS02325: BWI76_RS02325 - anaerobic C4-dicarboxylate transporter, at 477,994 to 479,295
_RS02325
BWI76_RS02330: BWI76_RS02330 - aspartate ammonia-lyase, at 479,414 to 480,850
_RS02330
Position (kb) 477
478
479
480 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 477.006 kb on - strand, within BWI76_RS02315 at 477.252 kb on + strand, within BWI76_RS02315 at 477.353 kb on - strand, within BWI76_RS02315 at 477.423 kb on + strand, within BWI76_RS02315 at 477.453 kb on + strand at 477.461 kb on - strand at 477.573 kb on - strand at 477.681 kb on + strand, within BWI76_RS02320 at 477.793 kb on - strand, within BWI76_RS02320 at 477.837 kb on + strand, within BWI76_RS02320 at 477.844 kb on - strand, within BWI76_RS02320 at 477.860 kb on + strand, within BWI76_RS02320 at 477.868 kb on - strand at 477.881 kb on - strand at 477.929 kb on + strand at 477.972 kb on - strand at 478.046 kb on - strand at 478.060 kb on + strand at 478.098 kb on + strand at 478.201 kb on - strand, within BWI76_RS02325 at 478.211 kb on - strand, within BWI76_RS02325 at 478.336 kb on + strand, within BWI76_RS02325 at 478.346 kb on + strand, within BWI76_RS02325 at 478.472 kb on + strand, within BWI76_RS02325 at 478.480 kb on - strand, within BWI76_RS02325 at 478.538 kb on - strand, within BWI76_RS02325 at 478.543 kb on + strand, within BWI76_RS02325 at 478.548 kb on + strand, within BWI76_RS02325 at 478.556 kb on - strand, within BWI76_RS02325 at 478.617 kb on - strand, within BWI76_RS02325 at 478.671 kb on - strand, within BWI76_RS02325 at 478.677 kb on - strand, within BWI76_RS02325 at 478.794 kb on + strand, within BWI76_RS02325 at 478.949 kb on - strand, within BWI76_RS02325 at 479.072 kb on - strand, within BWI76_RS02325 at 479.137 kb on + strand, within BWI76_RS02325 at 479.165 kb on - strand at 479.210 kb on - strand at 479.288 kb on - strand at 479.293 kb on + strand at 479.293 kb on - strand at 479.302 kb on - strand at 479.395 kb on - strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.441 kb on - strand at 479.476 kb on - strand at 479.502 kb on - strand at 479.523 kb on + strand at 479.588 kb on - strand, within BWI76_RS02330 at 479.610 kb on - strand, within BWI76_RS02330 at 479.889 kb on - strand, within BWI76_RS02330 at 479.970 kb on + strand, within BWI76_RS02330 at 480.014 kb on - strand, within BWI76_RS02330 at 480.112 kb on + strand, within BWI76_RS02330 at 480.120 kb on - strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.139 kb on - strand, within BWI76_RS02330
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 477,006 - BWI76_RS02315 0.66 -1.7 477,252 + BWI76_RS02315 0.80 +2.3 477,353 - BWI76_RS02315 0.86 +0.3 477,423 + BWI76_RS02315 0.90 -1.4 477,453 + -2.6 477,461 - +0.0 477,573 - -1.2 477,681 + BWI76_RS02320 0.32 -3.3 477,793 - BWI76_RS02320 0.67 +0.6 477,837 + BWI76_RS02320 0.81 +0.2 477,844 - BWI76_RS02320 0.83 +1.3 477,860 + BWI76_RS02320 0.88 +0.1 477,868 - +1.7 477,881 - -0.3 477,929 + -2.6 477,972 - +1.1 478,046 - -0.1 478,060 + +0.3 478,098 + -0.8 478,201 - BWI76_RS02325 0.16 +1.0 478,211 - BWI76_RS02325 0.17 +0.6 478,336 + BWI76_RS02325 0.26 +0.2 478,346 + BWI76_RS02325 0.27 -3.3 478,472 + BWI76_RS02325 0.37 +0.7 478,480 - BWI76_RS02325 0.37 +0.3 478,538 - BWI76_RS02325 0.42 -2.0 478,543 + BWI76_RS02325 0.42 +1.8 478,548 + BWI76_RS02325 0.43 +0.0 478,556 - BWI76_RS02325 0.43 +1.3 478,617 - BWI76_RS02325 0.48 +0.9 478,671 - BWI76_RS02325 0.52 +4.1 478,677 - BWI76_RS02325 0.52 -1.0 478,794 + BWI76_RS02325 0.61 +0.8 478,949 - BWI76_RS02325 0.73 +0.7 479,072 - BWI76_RS02325 0.83 +1.5 479,137 + BWI76_RS02325 0.88 +0.4 479,165 - -1.3 479,210 - +1.9 479,288 - -1.0 479,293 + -2.3 479,293 - +1.0 479,302 - -3.9 479,395 - +0.7 479,433 + -0.3 479,433 + -1.3 479,433 + -3.3 479,433 + +0.3 479,441 - -0.5 479,476 - +1.3 479,502 - -3.6 479,523 + -1.9 479,588 - BWI76_RS02330 0.12 -1.3 479,610 - BWI76_RS02330 0.14 +1.2 479,889 - BWI76_RS02330 0.33 +0.3 479,970 + BWI76_RS02330 0.39 -0.7 480,014 - BWI76_RS02330 0.42 +0.8 480,112 + BWI76_RS02330 0.49 -1.3 480,120 - BWI76_RS02330 0.49 +0.5 480,131 + BWI76_RS02330 0.50 +0.1 480,131 + BWI76_RS02330 0.50 -2.2 480,139 - BWI76_RS02330 0.50 -3.0
Or see this region's nucleotide sequence