Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02235 and BWI76_RS02240 are separated by 160 nucleotides BWI76_RS02240 and BWI76_RS02245 overlap by 6 nucleotides BWI76_RS02245 and BWI76_RS02250 are separated by 330 nucleotides
BWI76_RS02235: BWI76_RS02235 - VOC family protein, at 461,385 to 461,819
_RS02235
BWI76_RS02240: BWI76_RS02240 - alkylphosphonate utilization operon protein PhnA, at 461,980 to 462,315
_RS02240
BWI76_RS02245: BWI76_RS02245 - hypothetical protein, at 462,310 to 462,525
_RS02245
BWI76_RS02250: BWI76_RS02250 - proline/betaine transporter, at 462,856 to 464,358
_RS02250
Position (kb) 462
463 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 461.409 kb on - strand at 461.556 kb on + strand, within BWI76_RS02235 at 461.634 kb on + strand, within BWI76_RS02235 at 461.664 kb on - strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.751 kb on - strand, within BWI76_RS02235 at 461.774 kb on + strand, within BWI76_RS02235 at 461.777 kb on - strand at 461.800 kb on + strand at 461.809 kb on + strand at 461.815 kb on + strand at 461.824 kb on - strand at 461.828 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.854 kb on - strand at 461.861 kb on - strand at 461.861 kb on - strand at 461.895 kb on + strand at 461.895 kb on + strand at 461.900 kb on + strand at 461.901 kb on - strand at 461.908 kb on - strand at 461.914 kb on + strand at 461.919 kb on + strand at 461.919 kb on + strand at 461.921 kb on + strand at 461.921 kb on + strand at 461.927 kb on - strand at 462.021 kb on - strand, within BWI76_RS02240 at 462.040 kb on - strand, within BWI76_RS02240 at 462.055 kb on + strand at 462.056 kb on + strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.073 kb on + strand, within BWI76_RS02240 at 462.102 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.113 kb on + strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.144 kb on + strand, within BWI76_RS02240 at 462.202 kb on + strand, within BWI76_RS02240 at 462.204 kb on + strand, within BWI76_RS02240 at 462.205 kb on + strand, within BWI76_RS02240 at 462.212 kb on + strand, within BWI76_RS02240 at 462.213 kb on + strand, within BWI76_RS02240 at 462.218 kb on + strand, within BWI76_RS02240 at 462.220 kb on - strand, within BWI76_RS02240 at 462.221 kb on - strand, within BWI76_RS02240 at 462.223 kb on + strand, within BWI76_RS02240 at 462.226 kb on - strand, within BWI76_RS02240 at 462.226 kb on - strand at 462.228 kb on + strand, within BWI76_RS02240 at 462.260 kb on - strand, within BWI76_RS02240 at 462.273 kb on - strand, within BWI76_RS02240 at 462.274 kb on + strand, within BWI76_RS02240 at 462.299 kb on + strand at 462.317 kb on - strand at 462.343 kb on - strand, within BWI76_RS02245 at 462.343 kb on - strand, within BWI76_RS02245 at 462.376 kb on - strand, within BWI76_RS02245 at 462.380 kb on + strand, within BWI76_RS02245 at 462.382 kb on - strand, within BWI76_RS02245 at 462.435 kb on + strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.514 kb on + strand at 462.526 kb on + strand at 462.558 kb on - strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.613 kb on + strand at 462.613 kb on + strand at 462.656 kb on - strand at 462.664 kb on + strand at 462.680 kb on + strand at 462.680 kb on + strand at 462.683 kb on - strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.693 kb on + strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.698 kb on - strand at 462.698 kb on - strand at 462.699 kb on + strand at 462.706 kb on + strand at 462.708 kb on + strand at 462.714 kb on - strand at 462.718 kb on + strand at 462.727 kb on - strand at 462.727 kb on - strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.733 kb on + strand at 462.733 kb on + strand at 462.734 kb on + strand at 462.737 kb on - strand at 462.737 kb on - strand at 462.746 kb on - strand at 462.749 kb on + strand at 462.755 kb on + strand at 462.760 kb on + strand at 462.763 kb on - strand at 462.765 kb on + strand at 462.793 kb on - strand at 462.802 kb on - strand at 462.813 kb on + strand at 462.841 kb on - strand at 462.889 kb on + strand at 462.951 kb on + strand at 462.951 kb on + strand at 463.008 kb on + strand, within BWI76_RS02250 at 463.023 kb on + strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.059 kb on + strand, within BWI76_RS02250 at 463.117 kb on + strand, within BWI76_RS02250 at 463.259 kb on - strand, within BWI76_RS02250 at 463.276 kb on - strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.293 kb on - strand, within BWI76_RS02250 at 463.325 kb on - strand, within BWI76_RS02250 at 463.379 kb on + strand, within BWI76_RS02250 at 463.454 kb on + strand, within BWI76_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 461,409 - -0.8 461,556 + BWI76_RS02235 0.39 -4.5 461,634 + BWI76_RS02235 0.57 +0.1 461,664 - BWI76_RS02235 0.64 -2.0 461,738 + BWI76_RS02235 0.81 -0.8 461,738 + BWI76_RS02235 0.81 +0.9 461,738 + BWI76_RS02235 0.81 +1.7 461,746 - BWI76_RS02235 0.83 +0.5 461,746 - BWI76_RS02235 0.83 -0.7 461,746 - BWI76_RS02235 0.83 +1.3 461,746 - BWI76_RS02235 0.83 +0.3 461,751 - BWI76_RS02235 0.84 -1.0 461,774 + BWI76_RS02235 0.89 +0.3 461,777 - +0.4 461,800 + -1.0 461,809 + +0.7 461,815 + +1.1 461,824 - +1.0 461,828 - +1.3 461,830 - +0.7 461,830 - +0.4 461,830 - +1.7 461,830 - -2.9 461,853 + +0.6 461,853 + +0.7 461,853 + +1.3 461,854 - -2.9 461,861 - +1.4 461,861 - -1.5 461,895 + -0.3 461,895 + +1.1 461,900 + -0.4 461,901 - +1.6 461,908 - +0.6 461,914 + -1.9 461,919 + +0.3 461,919 + -0.3 461,921 + +1.0 461,921 + -0.3 461,927 - -1.3 462,021 - BWI76_RS02240 0.12 -2.3 462,040 - BWI76_RS02240 0.18 +0.5 462,055 + +1.3 462,056 + BWI76_RS02240 0.23 -0.2 462,064 - BWI76_RS02240 0.25 +0.4 462,064 - BWI76_RS02240 0.25 -3.3 462,064 - BWI76_RS02240 0.25 +0.4 462,064 - BWI76_RS02240 0.25 +0.8 462,064 - BWI76_RS02240 0.25 -0.3 462,064 - BWI76_RS02240 0.25 -0.7 462,064 - BWI76_RS02240 0.25 +1.5 462,073 + BWI76_RS02240 0.28 -0.2 462,102 + BWI76_RS02240 0.36 -2.3 462,112 + BWI76_RS02240 0.39 -0.7 462,112 + BWI76_RS02240 0.39 +0.2 462,113 + BWI76_RS02240 0.40 -1.5 462,120 - BWI76_RS02240 0.42 +1.1 462,120 - BWI76_RS02240 0.42 +0.3 462,120 - BWI76_RS02240 0.42 -3.3 462,121 - BWI76_RS02240 0.42 +0.7 462,121 - BWI76_RS02240 0.42 +0.7 462,144 + BWI76_RS02240 0.49 +5.0 462,202 + BWI76_RS02240 0.66 +0.7 462,204 + BWI76_RS02240 0.67 +0.3 462,205 + BWI76_RS02240 0.67 +2.2 462,212 + BWI76_RS02240 0.69 +0.7 462,213 + BWI76_RS02240 0.69 -2.8 462,218 + BWI76_RS02240 0.71 +0.3 462,220 - BWI76_RS02240 0.71 +0.3 462,221 - BWI76_RS02240 0.72 +0.0 462,223 + BWI76_RS02240 0.72 -0.1 462,226 - BWI76_RS02240 0.73 +1.4 462,226 - +1.3 462,228 + BWI76_RS02240 0.74 +0.7 462,260 - BWI76_RS02240 0.83 +1.4 462,273 - BWI76_RS02240 0.87 -1.8 462,274 + BWI76_RS02240 0.88 +1.3 462,299 + +0.7 462,317 - -0.5 462,343 - BWI76_RS02245 0.15 +1.4 462,343 - BWI76_RS02245 0.15 +1.4 462,376 - BWI76_RS02245 0.31 -1.4 462,380 + BWI76_RS02245 0.32 +0.8 462,382 - BWI76_RS02245 0.33 +0.2 462,435 + BWI76_RS02245 0.58 +1.2 462,443 - BWI76_RS02245 0.62 -1.5 462,443 - BWI76_RS02245 0.62 +1.7 462,443 - BWI76_RS02245 0.62 +0.8 462,514 + +0.3 462,526 + +0.6 462,558 - -1.6 462,566 + +0.1 462,566 + +0.4 462,566 + +0.5 462,566 + +0.9 462,566 + -3.1 462,566 + +0.5 462,566 + +3.1 462,566 + +1.4 462,566 + -0.3 462,566 + +0.9 462,566 + -2.9 462,566 + -0.8 462,566 + +0.5 462,566 + +0.3 462,566 + +3.0 462,566 + +0.7 462,566 + -1.1 462,566 + +2.2 462,566 + +1.3 462,574 - +0.4 462,574 - -1.0 462,574 - -0.7 462,574 - +0.9 462,574 - +0.7 462,574 - -0.7 462,574 - +0.4 462,574 - +0.9 462,574 - +1.3 462,574 - -3.0 462,574 - -0.7 462,574 - +1.4 462,574 - +1.3 462,574 - -3.2 462,574 - -2.8 462,574 - +0.7 462,613 + -1.0 462,613 + -1.0 462,656 - -2.7 462,664 + -2.4 462,680 + +0.6 462,680 + -1.3 462,683 - -1.5 462,685 + +0.3 462,685 + -3.8 462,685 + +0.3 462,685 + -2.9 462,690 + +0.3 462,690 + -0.3 462,690 + -1.0 462,690 + +1.0 462,690 + -1.3 462,690 + -0.4 462,690 + +0.5 462,690 + +0.6 462,690 + +0.8 462,690 + -3.4 462,690 + -1.3 462,693 + +0.1 462,693 - +1.4 462,693 - -0.7 462,693 - +1.2 462,693 - -0.5 462,698 - -2.9 462,698 - +1.3 462,699 + +1.1 462,706 + -2.3 462,708 + +0.1 462,714 - -2.2 462,718 + -1.0 462,727 - +1.3 462,727 - -1.3 462,729 + +1.2 462,729 + -0.8 462,729 + +0.7 462,733 + +0.3 462,733 + -2.6 462,734 + +1.4 462,737 - -2.4 462,737 - +0.3 462,746 - -0.0 462,749 + -1.5 462,755 + +1.3 462,760 + -0.6 462,763 - -0.3 462,765 + -3.5 462,793 - -0.2 462,802 - -0.3 462,813 + -0.0 462,841 - +1.3 462,889 + +0.2 462,951 + -0.6 462,951 + +1.1 463,008 + BWI76_RS02250 0.10 +2.0 463,023 + BWI76_RS02250 0.11 -0.3 463,050 - BWI76_RS02250 0.13 +0.7 463,050 - BWI76_RS02250 0.13 -1.2 463,059 + BWI76_RS02250 0.14 +0.4 463,117 + BWI76_RS02250 0.17 +0.9 463,259 - BWI76_RS02250 0.27 -0.6 463,276 - BWI76_RS02250 0.28 -2.5 463,285 + BWI76_RS02250 0.29 -4.3 463,285 + BWI76_RS02250 0.29 -3.3 463,293 - BWI76_RS02250 0.29 +1.2 463,325 - BWI76_RS02250 0.31 -0.9 463,379 + BWI76_RS02250 0.35 +0.9 463,454 + BWI76_RS02250 0.40 +0.8
Or see this region's nucleotide sequence