Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01685 and BWI76_RS01690 are separated by 185 nucleotides BWI76_RS01690 and BWI76_RS01695 are separated by 261 nucleotides
BWI76_RS01685: BWI76_RS01685 - methionine synthase, at 343,151 to 346,834
_RS01685
BWI76_RS01690: BWI76_RS01690 - membrane protein, at 347,020 to 348,651
_RS01690
BWI76_RS01695: BWI76_RS01695 - IS110 family transposase, at 348,913 to 349,893
_RS01695
Position (kb) 347
348
349 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 346.086 kb on - strand, within BWI76_RS01685 at 346.116 kb on - strand, within BWI76_RS01685 at 346.329 kb on + strand, within BWI76_RS01685 at 346.413 kb on - strand, within BWI76_RS01685 at 346.575 kb on + strand at 346.610 kb on - strand at 346.756 kb on - strand at 346.872 kb on + strand at 346.909 kb on + strand at 346.909 kb on + strand at 346.910 kb on - strand at 346.983 kb on - strand at 347.029 kb on + strand at 347.043 kb on - strand at 347.097 kb on - strand at 347.097 kb on - strand at 347.121 kb on - strand at 347.263 kb on + strand, within BWI76_RS01690 at 347.321 kb on + strand, within BWI76_RS01690 at 347.478 kb on - strand, within BWI76_RS01690 at 347.495 kb on - strand, within BWI76_RS01690 at 347.657 kb on - strand, within BWI76_RS01690 at 347.813 kb on - strand, within BWI76_RS01690 at 347.927 kb on - strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 348.590 kb on + strand at 348.598 kb on - strand at 348.607 kb on - strand at 348.669 kb on + strand at 348.971 kb on + strand at 349.010 kb on + strand at 349.027 kb on + strand, within BWI76_RS01695 at 349.073 kb on + strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.093 kb on + strand, within BWI76_RS01695 at 349.097 kb on + strand, within BWI76_RS01695 at 349.102 kb on + strand, within BWI76_RS01695 at 349.492 kb on - strand, within BWI76_RS01695 at 349.569 kb on + strand, within BWI76_RS01695 at 349.607 kb on + strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 346,086 - BWI76_RS01685 0.80 -0.1 346,116 - BWI76_RS01685 0.80 -2.1 346,329 + BWI76_RS01685 0.86 -2.7 346,413 - BWI76_RS01685 0.89 -4.5 346,575 + -1.0 346,610 - -0.1 346,756 - -1.2 346,872 + -1.2 346,909 + +1.3 346,909 + -0.1 346,910 - +0.9 346,983 - -0.9 347,029 + -1.6 347,043 - +2.0 347,097 - +0.1 347,097 - +0.6 347,121 - +0.4 347,263 + BWI76_RS01690 0.15 +1.9 347,321 + BWI76_RS01690 0.18 +0.7 347,478 - BWI76_RS01690 0.28 +1.4 347,495 - BWI76_RS01690 0.29 +0.3 347,657 - BWI76_RS01690 0.39 +0.8 347,813 - BWI76_RS01690 0.49 -2.0 347,927 - BWI76_RS01690 0.56 -2.7 347,994 + BWI76_RS01690 0.60 +1.2 347,994 + BWI76_RS01690 0.60 +0.6 348,590 + +0.3 348,598 - -3.3 348,607 - +1.4 348,669 + -2.0 348,971 + +1.6 349,010 + -1.6 349,027 + BWI76_RS01695 0.12 +0.7 349,073 + BWI76_RS01695 0.16 +0.6 349,081 - BWI76_RS01695 0.17 +1.5 349,081 - BWI76_RS01695 0.17 +0.9 349,081 - BWI76_RS01695 0.17 -1.8 349,081 - BWI76_RS01695 0.17 -0.8 349,093 + BWI76_RS01695 0.18 +0.4 349,097 + BWI76_RS01695 0.19 +0.6 349,102 + BWI76_RS01695 0.19 +0.2 349,492 - BWI76_RS01695 0.59 +0.1 349,569 + BWI76_RS01695 0.67 +0.0 349,607 + BWI76_RS01695 0.71 +2.6 349,633 - BWI76_RS01695 0.73 +0.4 349,633 - BWI76_RS01695 0.73 +1.1
Or see this region's nucleotide sequence