Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01060 and BWI76_RS01065 are separated by 134 nucleotides BWI76_RS01065 and BWI76_RS01070 are separated by 341 nucleotides
BWI76_RS01060: BWI76_RS01060 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 216,812 to 218,311
_RS01060
BWI76_RS01065: BWI76_RS01065 - ATP-dependent RNA helicase RhlB, at 218,446 to 219,711
_RS01065
BWI76_RS01070: BWI76_RS01070 - thiol reductase thioredoxin, at 220,053 to 220,382
_RS01070
Position (kb) 218
219
220 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 217.717 kb on + strand, within BWI76_RS01060 at 217.720 kb on - strand, within BWI76_RS01060 at 217.835 kb on - strand, within BWI76_RS01060 at 217.993 kb on - strand, within BWI76_RS01060 at 218.297 kb on - strand at 218.299 kb on + strand at 218.321 kb on - strand at 218.332 kb on + strand at 218.340 kb on - strand at 218.448 kb on + strand at 218.451 kb on - strand at 218.533 kb on - strand at 218.533 kb on - strand at 218.965 kb on + strand, within BWI76_RS01065 at 218.966 kb on + strand, within BWI76_RS01065 at 219.065 kb on + strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.254 kb on - strand, within BWI76_RS01065 at 219.323 kb on + strand, within BWI76_RS01065 at 219.377 kb on - strand, within BWI76_RS01065 at 219.742 kb on - strand at 219.863 kb on + strand at 219.901 kb on + strand at 220.103 kb on + strand, within BWI76_RS01070 at 220.103 kb on + strand, within BWI76_RS01070 at 220.103 kb on + strand, within BWI76_RS01070 at 220.111 kb on - strand, within BWI76_RS01070 at 220.125 kb on - strand, within BWI76_RS01070 at 220.125 kb on - strand, within BWI76_RS01070 at 220.155 kb on - strand, within BWI76_RS01070 at 220.226 kb on + strand, within BWI76_RS01070 at 220.337 kb on + strand, within BWI76_RS01070 at 220.344 kb on + strand, within BWI76_RS01070 at 220.345 kb on - strand, within BWI76_RS01070 at 220.345 kb on - strand, within BWI76_RS01070 at 220.414 kb on - strand at 220.433 kb on - strand at 220.448 kb on + strand at 220.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 217,717 + BWI76_RS01060 0.60 -0.4 217,720 - BWI76_RS01060 0.61 -1.2 217,835 - BWI76_RS01060 0.68 +0.4 217,993 - BWI76_RS01060 0.79 -1.7 218,297 - +0.1 218,299 + -5.0 218,321 - +0.4 218,332 + -0.0 218,340 - -2.4 218,448 + +0.4 218,451 - +0.6 218,533 - +0.5 218,533 - -2.0 218,965 + BWI76_RS01065 0.41 -1.1 218,966 + BWI76_RS01065 0.41 -1.8 219,065 + BWI76_RS01065 0.49 -2.0 219,124 - BWI76_RS01065 0.54 -2.0 219,124 - BWI76_RS01065 0.54 +0.4 219,254 - BWI76_RS01065 0.64 +0.0 219,323 + BWI76_RS01065 0.69 +1.4 219,377 - BWI76_RS01065 0.74 -2.9 219,742 - +0.7 219,863 + -3.0 219,901 + -1.3 220,103 + BWI76_RS01070 0.15 +0.3 220,103 + BWI76_RS01070 0.15 -1.6 220,103 + BWI76_RS01070 0.15 -1.9 220,111 - BWI76_RS01070 0.18 +2.1 220,125 - BWI76_RS01070 0.22 +1.1 220,125 - BWI76_RS01070 0.22 -0.9 220,155 - BWI76_RS01070 0.31 -0.9 220,226 + BWI76_RS01070 0.52 -1.4 220,337 + BWI76_RS01070 0.86 -2.6 220,344 + BWI76_RS01070 0.88 +0.2 220,345 - BWI76_RS01070 0.88 -2.0 220,345 - BWI76_RS01070 0.88 -0.8 220,414 - -2.6 220,433 - -0.5 220,448 + -0.8 220,504 + -0.3
Or see this region's nucleotide sequence