Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01020 and BWI76_RS01025 are separated by 3 nucleotides BWI76_RS01025 and BWI76_RS01030 overlap by 17 nucleotides
BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511
_RS01020
BWI76_RS01025: BWI76_RS01025 - hypothetical protein, at 209,515 to 210,477
_RS01025
BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876
_RS01030
Position (kb) 209
210
211 Strain fitness (log2 ratio) -2
-1
0
1
2 at 209.478 kb on + strand at 209.635 kb on + strand, within BWI76_RS01025 at 209.705 kb on + strand, within BWI76_RS01025 at 209.713 kb on - strand, within BWI76_RS01025 at 209.829 kb on - strand, within BWI76_RS01025 at 209.848 kb on + strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 210.021 kb on + strand, within BWI76_RS01025 at 210.031 kb on + strand, within BWI76_RS01025 at 210.034 kb on - strand, within BWI76_RS01025 at 210.065 kb on + strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.462 kb on - strand at 210.549 kb on + strand at 210.649 kb on + strand, within BWI76_RS01030 at 210.652 kb on - strand, within BWI76_RS01030 at 210.662 kb on - strand, within BWI76_RS01030 at 210.677 kb on + strand, within BWI76_RS01030 at 210.757 kb on + strand, within BWI76_RS01030 at 211.104 kb on + strand, within BWI76_RS01030 at 211.256 kb on - strand, within BWI76_RS01030 at 211.441 kb on + strand, within BWI76_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 209,478 + +0.2 209,635 + BWI76_RS01025 0.12 -1.4 209,705 + BWI76_RS01025 0.20 -2.0 209,713 - BWI76_RS01025 0.21 +1.4 209,829 - BWI76_RS01025 0.33 +0.2 209,848 + BWI76_RS01025 0.35 +2.4 209,865 - BWI76_RS01025 0.36 +1.0 209,865 - BWI76_RS01025 0.36 +0.9 209,865 - BWI76_RS01025 0.36 -2.5 210,021 + BWI76_RS01025 0.53 +0.2 210,031 + BWI76_RS01025 0.54 +0.1 210,034 - BWI76_RS01025 0.54 +0.9 210,065 + BWI76_RS01025 0.57 -0.1 210,128 - BWI76_RS01025 0.64 +0.7 210,128 - BWI76_RS01025 0.64 +1.3 210,462 - +1.4 210,549 + +0.3 210,649 + BWI76_RS01030 0.13 +2.6 210,652 - BWI76_RS01030 0.13 +0.5 210,662 - BWI76_RS01030 0.14 +0.7 210,677 + BWI76_RS01030 0.15 -0.4 210,757 + BWI76_RS01030 0.21 -0.2 211,104 + BWI76_RS01030 0.45 +0.4 211,256 - BWI76_RS01030 0.56 +0.3 211,441 + BWI76_RS01030 0.69 +1.1
Or see this region's nucleotide sequence