Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotides BWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides BWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides
BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033
_RS00960
BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287
_RS00965
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
Position (kb) 197
198
199 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 196.541 kb on - strand, within BWI76_RS00960 at 196.597 kb on + strand at 196.605 kb on - strand, within BWI76_RS00960 at 196.754 kb on + strand, within BWI76_RS00960 at 196.869 kb on + strand at 196.933 kb on + strand at 196.941 kb on - strand at 196.973 kb on + strand at 196.981 kb on - strand at 197.236 kb on - strand, within BWI76_RS00965 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970 at 198.044 kb on + strand, within BWI76_RS00970 at 198.112 kb on + strand, within BWI76_RS00970 at 198.168 kb on + strand at 198.297 kb on + strand at 198.442 kb on + strand at 198.442 kb on + strand at 198.543 kb on + strand, within BWI76_RS00975 at 198.551 kb on - strand, within BWI76_RS00975 at 198.608 kb on + strand, within BWI76_RS00975 at 198.636 kb on - strand, within BWI76_RS00975 at 198.648 kb on + strand, within BWI76_RS00975 at 198.849 kb on + strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 199.017 kb on - strand, within BWI76_RS00975 at 199.040 kb on + strand, within BWI76_RS00975 at 199.043 kb on - strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 196,541 - BWI76_RS00960 0.70 -2.7 196,597 + +1.5 196,605 - BWI76_RS00960 0.74 -3.8 196,754 + BWI76_RS00960 0.83 +0.1 196,869 + +0.3 196,933 + -0.2 196,941 - -5.3 196,973 + +0.4 196,981 - -3.7 197,236 - BWI76_RS00965 0.80 -2.8 197,330 - -1.4 197,383 + -0.5 197,399 - BWI76_RS00970 0.10 -4.3 197,484 + BWI76_RS00970 0.19 -2.8 197,484 + BWI76_RS00970 0.19 +1.4 197,540 + BWI76_RS00970 0.25 -4.2 197,540 + BWI76_RS00970 0.25 -2.7 197,686 + BWI76_RS00970 0.41 -0.9 198,044 + BWI76_RS00970 0.80 -1.7 198,112 + BWI76_RS00970 0.87 -0.3 198,168 + +0.3 198,297 + -4.7 198,442 + -3.5 198,442 + -6.2 198,543 + BWI76_RS00975 0.13 -1.5 198,551 - BWI76_RS00975 0.14 -3.3 198,608 + BWI76_RS00975 0.17 -4.0 198,636 - BWI76_RS00975 0.18 -5.7 198,648 + BWI76_RS00975 0.19 -4.7 198,849 + BWI76_RS00975 0.30 -2.2 198,850 - BWI76_RS00975 0.30 -6.4 198,850 - BWI76_RS00975 0.30 -1.8 199,017 - BWI76_RS00975 0.39 -3.9 199,040 + BWI76_RS00975 0.40 -2.6 199,043 - BWI76_RS00975 0.40 -5.3 199,226 + BWI76_RS00975 0.50 -5.7 199,226 + BWI76_RS00975 0.50 +0.5 199,226 + BWI76_RS00975 0.50 -4.2 199,226 + BWI76_RS00975 0.50 +0.2
Or see this region's nucleotide sequence