Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00290 and BWI76_RS00295 are separated by 12 nucleotides BWI76_RS00295 and BWI76_RS00300 overlap by 4 nucleotides BWI76_RS00300 and BWI76_RS00305 are separated by 3 nucleotides
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 60,235 to 61,164
_RS00290
BWI76_RS00295: BWI76_RS00295 - transcriptional regulator RbsR, at 61,177 to 62,160
_RS00295
BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554
_RS00300
BWI76_RS00305: BWI76_RS00305 - GntR family transcriptional regulator, at 63,558 to 64,268
_RS00305
Position (kb) 62
63
64 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 61.165 kb on + strand at 61.173 kb on - strand at 61.431 kb on + strand, within BWI76_RS00295 at 61.433 kb on + strand, within BWI76_RS00295 at 61.439 kb on + strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.447 kb on - strand, within BWI76_RS00295 at 61.457 kb on - strand, within BWI76_RS00295 at 61.580 kb on - strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.968 kb on - strand, within BWI76_RS00295 at 62.007 kb on + strand, within BWI76_RS00295 at 62.102 kb on + strand at 62.115 kb on - strand at 62.137 kb on - strand at 62.143 kb on - strand at 62.163 kb on - strand at 62.169 kb on - strand at 62.273 kb on + strand at 62.429 kb on + strand, within BWI76_RS00300 at 62.470 kb on + strand, within BWI76_RS00300 at 62.584 kb on - strand, within BWI76_RS00300 at 62.599 kb on - strand, within BWI76_RS00300 at 62.633 kb on + strand, within BWI76_RS00300 at 62.673 kb on + strand, within BWI76_RS00300 at 62.730 kb on - strand, within BWI76_RS00300 at 62.814 kb on - strand, within BWI76_RS00300 at 63.029 kb on - strand, within BWI76_RS00300 at 63.075 kb on + strand, within BWI76_RS00300 at 63.088 kb on - strand, within BWI76_RS00300 at 63.098 kb on - strand, within BWI76_RS00300 at 63.110 kb on - strand, within BWI76_RS00300 at 63.115 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.130 kb on - strand, within BWI76_RS00300 at 63.404 kb on - strand, within BWI76_RS00300 at 63.429 kb on + strand at 63.442 kb on - strand at 63.460 kb on + strand at 63.519 kb on - strand at 63.526 kb on + strand at 63.636 kb on + strand, within BWI76_RS00305 at 63.668 kb on + strand, within BWI76_RS00305 at 63.690 kb on - strand, within BWI76_RS00305 at 63.706 kb on + strand, within BWI76_RS00305 at 63.722 kb on - strand, within BWI76_RS00305 at 63.811 kb on - strand, within BWI76_RS00305 at 63.862 kb on + strand, within BWI76_RS00305 at 63.862 kb on - strand, within BWI76_RS00305 at 63.870 kb on - strand, within BWI76_RS00305 at 63.923 kb on + strand, within BWI76_RS00305 at 63.928 kb on + strand, within BWI76_RS00305 at 63.931 kb on - strand, within BWI76_RS00305 at 63.941 kb on - strand, within BWI76_RS00305 at 63.962 kb on + strand, within BWI76_RS00305 at 63.962 kb on - strand, within BWI76_RS00305 at 63.980 kb on - strand, within BWI76_RS00305 at 64.063 kb on + strand, within BWI76_RS00305 at 64.076 kb on + strand, within BWI76_RS00305 at 64.117 kb on - strand, within BWI76_RS00305 at 64.150 kb on - strand, within BWI76_RS00305 at 64.212 kb on - strand at 64.233 kb on - strand at 64.251 kb on - strand at 64.274 kb on - strand at 64.293 kb on + strand at 64.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 61,165 + +1.2 61,173 - +0.3 61,431 + BWI76_RS00295 0.26 +0.7 61,433 + BWI76_RS00295 0.26 -3.1 61,439 + BWI76_RS00295 0.27 +1.2 61,441 - BWI76_RS00295 0.27 +0.5 61,441 - BWI76_RS00295 0.27 +0.0 61,447 - BWI76_RS00295 0.27 +1.0 61,457 - BWI76_RS00295 0.28 +0.9 61,580 - BWI76_RS00295 0.41 -3.9 61,960 + BWI76_RS00295 0.80 +1.0 61,960 + BWI76_RS00295 0.80 +0.1 61,968 - BWI76_RS00295 0.80 +1.5 62,007 + BWI76_RS00295 0.84 +1.0 62,102 + +1.3 62,115 - -1.2 62,137 - +0.2 62,143 - +1.2 62,163 - +0.4 62,169 - -0.1 62,273 + -0.2 62,429 + BWI76_RS00300 0.19 +0.6 62,470 + BWI76_RS00300 0.22 +0.0 62,584 - BWI76_RS00300 0.31 -2.8 62,599 - BWI76_RS00300 0.32 +1.2 62,633 + BWI76_RS00300 0.34 +1.6 62,673 + BWI76_RS00300 0.37 +0.5 62,730 - BWI76_RS00300 0.41 +0.3 62,814 - BWI76_RS00300 0.47 -4.1 63,029 - BWI76_RS00300 0.62 -0.1 63,075 + BWI76_RS00300 0.66 +1.0 63,088 - BWI76_RS00300 0.67 +0.2 63,098 - BWI76_RS00300 0.67 +1.4 63,110 - BWI76_RS00300 0.68 +1.3 63,115 - BWI76_RS00300 0.69 -1.9 63,129 - BWI76_RS00300 0.70 +1.9 63,129 - BWI76_RS00300 0.70 +1.5 63,130 - BWI76_RS00300 0.70 -0.1 63,404 - BWI76_RS00300 0.89 +0.7 63,429 + +0.6 63,442 - -1.5 63,460 + +1.7 63,519 - +0.1 63,526 + -2.7 63,636 + BWI76_RS00305 0.11 -0.6 63,668 + BWI76_RS00305 0.15 -3.5 63,690 - BWI76_RS00305 0.19 +0.5 63,706 + BWI76_RS00305 0.21 -0.1 63,722 - BWI76_RS00305 0.23 +1.8 63,811 - BWI76_RS00305 0.36 +0.5 63,862 + BWI76_RS00305 0.43 +0.5 63,862 - BWI76_RS00305 0.43 -0.6 63,870 - BWI76_RS00305 0.44 -4.1 63,923 + BWI76_RS00305 0.51 +1.1 63,928 + BWI76_RS00305 0.52 -1.2 63,931 - BWI76_RS00305 0.52 +1.0 63,941 - BWI76_RS00305 0.54 -0.3 63,962 + BWI76_RS00305 0.57 -1.9 63,962 - BWI76_RS00305 0.57 +0.5 63,980 - BWI76_RS00305 0.59 +0.8 64,063 + BWI76_RS00305 0.71 +1.8 64,076 + BWI76_RS00305 0.73 -0.4 64,117 - BWI76_RS00305 0.79 +0.5 64,150 - BWI76_RS00305 0.83 +0.5 64,212 - +0.5 64,233 - +2.0 64,251 - -2.3 64,274 - +0.6 64,293 + +0.3 64,433 - +0.3
Or see this region's nucleotide sequence