Experiment: MoLS4 with TEMED 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aat and DVU1604 are separated by 6 nucleotides DVU1604 and uvrB are separated by 92 nucleotides uvrB and DVU1606 overlap by 4 nucleotides
DVU1603: aat - leucyl/phenylalanyl-tRNA--protein transferase (TIGR), at 1,685,506 to 1,686,210
aat
DVU1604: DVU1604 - hypothetical protein (TIGR), at 1,686,217 to 1,686,702
DVU1604
DVU1605: uvrB - excinuclease ABC, B subunit (TIGR), at 1,686,795 to 1,688,828
uvrB
DVU1606: DVU1606 - potassium uptake protein, TrkA family (TIGR), at 1,688,825 to 1,689,877
DVU1606
Position (kb)
1686
1687
1688
1689 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1685.837 kb on - strand, within aat at 1685.837 kb on - strand, within aat at 1685.867 kb on + strand, within aat at 1685.883 kb on + strand, within aat at 1685.940 kb on + strand, within aat at 1685.940 kb on + strand, within aat at 1685.948 kb on - strand, within aat at 1685.953 kb on - strand, within aat at 1685.953 kb on - strand, within aat at 1686.026 kb on - strand, within aat at 1686.103 kb on + strand, within aat at 1686.248 kb on - strand at 1686.275 kb on - strand, within DVU1604 at 1686.293 kb on - strand, within DVU1604 at 1686.319 kb on + strand, within DVU1604 at 1686.549 kb on + strand, within DVU1604 at 1686.549 kb on + strand, within DVU1604 at 1686.549 kb on + strand, within DVU1604 at 1686.597 kb on - strand, within DVU1604 at 1686.647 kb on + strand, within DVU1604 at 1686.655 kb on - strand at 1686.808 kb on + strand at 1686.838 kb on + strand at 1686.838 kb on + strand at 1686.883 kb on + strand at 1686.891 kb on - strand at 1686.944 kb on + strand at 1686.952 kb on - strand at 1686.970 kb on + strand at 1686.970 kb on + strand at 1686.983 kb on - strand at 1687.163 kb on + strand, within uvrB at 1687.185 kb on + strand, within uvrB at 1687.332 kb on - strand, within uvrB at 1687.474 kb on + strand, within uvrB at 1687.482 kb on - strand, within uvrB at 1687.497 kb on + strand, within uvrB at 1687.505 kb on - strand, within uvrB at 1687.596 kb on - strand, within uvrB at 1687.651 kb on + strand, within uvrB at 1687.664 kb on - strand, within uvrB at 1687.703 kb on + strand, within uvrB at 1687.821 kb on - strand, within uvrB at 1687.835 kb on + strand, within uvrB at 1687.843 kb on - strand, within uvrB at 1687.847 kb on + strand, within uvrB at 1687.855 kb on - strand, within uvrB at 1687.855 kb on - strand, within uvrB at 1687.952 kb on + strand, within uvrB at 1687.973 kb on - strand, within uvrB at 1687.975 kb on + strand, within uvrB at 1688.038 kb on + strand, within uvrB at 1688.073 kb on + strand, within uvrB at 1688.180 kb on - strand, within uvrB at 1688.234 kb on + strand, within uvrB at 1688.270 kb on - strand, within uvrB at 1688.297 kb on + strand, within uvrB at 1688.305 kb on + strand, within uvrB at 1688.402 kb on + strand, within uvrB at 1688.402 kb on + strand, within uvrB at 1688.443 kb on + strand, within uvrB at 1688.552 kb on + strand, within uvrB at 1688.609 kb on + strand, within uvrB at 1688.873 kb on - strand at 1689.027 kb on + strand, within DVU1606 at 1689.083 kb on - strand, within DVU1606 at 1689.135 kb on - strand, within DVU1606 at 1689.160 kb on - strand, within DVU1606 at 1689.181 kb on + strand, within DVU1606 at 1689.195 kb on - strand, within DVU1606 at 1689.240 kb on + strand, within DVU1606 at 1689.324 kb on + strand, within DVU1606 at 1689.382 kb on + strand, within DVU1606 at 1689.464 kb on - strand, within DVU1606 at 1689.687 kb on + strand, within DVU1606 at 1689.692 kb on - strand, within DVU1606 at 1689.731 kb on + strand, within DVU1606 at 1689.733 kb on + strand, within DVU1606
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with TEMED 20mM (N) remove 1,685,837 - aat DVU1603 0.47 +0.3 1,685,837 - aat DVU1603 0.47 -0.0 1,685,867 + aat DVU1603 0.51 +0.2 1,685,883 + aat DVU1603 0.53 -1.8 1,685,940 + aat DVU1603 0.62 -0.0 1,685,940 + aat DVU1603 0.62 -0.7 1,685,948 - aat DVU1603 0.63 -0.9 1,685,953 - aat DVU1603 0.63 -0.2 1,685,953 - aat DVU1603 0.63 -0.3 1,686,026 - aat DVU1603 0.74 +0.1 1,686,103 + aat DVU1603 0.85 -0.1 1,686,248 - +0.1 1,686,275 - DVU1604 0.12 +2.3 1,686,293 - DVU1604 0.16 +0.0 1,686,319 + DVU1604 0.21 -0.1 1,686,549 + DVU1604 0.68 +0.3 1,686,549 + DVU1604 0.68 -0.2 1,686,549 + DVU1604 0.68 -0.6 1,686,597 - DVU1604 0.78 -0.1 1,686,647 + DVU1604 0.88 -0.4 1,686,655 - +1.7 1,686,808 + -1.2 1,686,838 + -2.5 1,686,838 + +0.2 1,686,883 + -1.5 1,686,891 - +0.3 1,686,944 + -1.0 1,686,952 - +1.5 1,686,970 + -1.1 1,686,970 + -0.1 1,686,983 - -0.6 1,687,163 + uvrB DVU1605 0.18 -1.1 1,687,185 + uvrB DVU1605 0.19 -1.5 1,687,332 - uvrB DVU1605 0.26 -0.1 1,687,474 + uvrB DVU1605 0.33 -3.5 1,687,482 - uvrB DVU1605 0.34 -1.5 1,687,497 + uvrB DVU1605 0.35 -2.6 1,687,505 - uvrB DVU1605 0.35 -1.8 1,687,596 - uvrB DVU1605 0.39 -0.8 1,687,651 + uvrB DVU1605 0.42 -1.3 1,687,664 - uvrB DVU1605 0.43 -1.5 1,687,703 + uvrB DVU1605 0.45 -3.0 1,687,821 - uvrB DVU1605 0.50 -1.2 1,687,835 + uvrB DVU1605 0.51 -2.1 1,687,843 - uvrB DVU1605 0.52 -0.9 1,687,847 + uvrB DVU1605 0.52 -1.6 1,687,855 - uvrB DVU1605 0.52 -1.1 1,687,855 - uvrB DVU1605 0.52 -2.3 1,687,952 + uvrB DVU1605 0.57 -1.7 1,687,973 - uvrB DVU1605 0.58 -1.3 1,687,975 + uvrB DVU1605 0.58 -0.5 1,688,038 + uvrB DVU1605 0.61 -0.9 1,688,073 + uvrB DVU1605 0.63 -2.4 1,688,180 - uvrB DVU1605 0.68 +1.5 1,688,234 + uvrB DVU1605 0.71 -2.6 1,688,270 - uvrB DVU1605 0.73 -1.6 1,688,297 + uvrB DVU1605 0.74 -1.5 1,688,305 + uvrB DVU1605 0.74 -5.8 1,688,402 + uvrB DVU1605 0.79 -1.3 1,688,402 + uvrB DVU1605 0.79 -1.3 1,688,443 + uvrB DVU1605 0.81 -1.2 1,688,552 + uvrB DVU1605 0.86 -1.3 1,688,609 + uvrB DVU1605 0.89 -0.2 1,688,873 - -0.2 1,689,027 + DVU1606 0.19 +1.5 1,689,083 - DVU1606 0.25 +0.2 1,689,135 - DVU1606 0.29 -0.9 1,689,160 - DVU1606 0.32 -0.5 1,689,181 + DVU1606 0.34 +0.4 1,689,195 - DVU1606 0.35 -0.8 1,689,240 + DVU1606 0.39 +0.0 1,689,324 + DVU1606 0.47 +1.4 1,689,382 + DVU1606 0.53 +1.2 1,689,464 - DVU1606 0.61 +0.9 1,689,687 + DVU1606 0.82 +0.4 1,689,692 - DVU1606 0.82 +0.5 1,689,731 + DVU1606 0.86 +1.3 1,689,733 + DVU1606 0.86 -0.4
Or see this region's nucleotide sequence