Experiment: MoLS4 with TEMED 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glmM and galU are separated by 37 nucleotides galU and priA are separated by 75 nucleotides priA and DORF34036 are separated by 5 nucleotides DORF34036 and DVU1285 are separated by 67 nucleotides DVU1285 and DsrP are separated by 187 nucleotides
DVU1282: glmM - phosphoglucosamine mutase (TIGR), at 1,374,532 to 1,375,884
glmM
DVU1283: galU - UTP-glucose-1-phosphate uridylyltransferase (TIGR), at 1,375,922 to 1,376,800
galU
DVU1284: priA - primosomal protein n (TIGR), at 1,376,876 to 1,379,254
priA
DORF34036: DORF34036 - no description, at 1,379,260 to 1,379,436
DORF34036
DVU1285: DVU1285 - response regulator (TIGR), at 1,379,504 to 1,379,896
DVU1285
DVU1286: DsrP - Integral membrane protein (Shelley Haveman), at 1,380,084 to 1,381,247
DsrP
Position (kb)
1376
1377
1378
1379
1380 Strain fitness (log2 ratio)
-2
-1
0
1 at 1375.886 kb on + strand at 1375.926 kb on - strand at 1375.961 kb on + strand at 1376.137 kb on + strand, within galU at 1376.380 kb on + strand, within galU at 1376.524 kb on - strand, within galU at 1376.643 kb on + strand, within galU at 1376.788 kb on - strand at 1376.833 kb on + strand at 1379.403 kb on - strand, within DORF34036 at 1379.493 kb on - strand at 1379.598 kb on - strand, within DVU1285 at 1379.721 kb on - strand, within DVU1285 at 1379.831 kb on + strand, within DVU1285 at 1379.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with TEMED 20mM (N) remove 1,375,886 + -1.4 1,375,926 - -2.0 1,375,961 + -0.5 1,376,137 + galU DVU1283 0.24 -0.5 1,376,380 + galU DVU1283 0.52 +0.2 1,376,524 - galU DVU1283 0.68 -0.3 1,376,643 + galU DVU1283 0.82 -1.0 1,376,788 - +0.1 1,376,833 + -1.2 1,379,403 - DORF34036 0.81 -2.0 1,379,493 - +0.4 1,379,598 - DVU1285 0.24 -0.7 1,379,721 - DVU1285 0.55 -1.1 1,379,831 + DVU1285 0.83 -0.2 1,379,997 - -0.4
Or see this region's nucleotide sequence