Experiment: MoLS4 with TEMED 20mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusA and DVU0511 are separated by 40 nucleotides DVU0511 and flgF are separated by 341 nucleotides flgF and flgG are separated by 56 nucleotides flgG and DVU0514 are separated by 49 nucleotides
DVU0510: nusA - N utilization substance protein A (TIGR), at 583,521 to 584,807
nusA
DVU0511: DVU0511 - conserved hypothetical protein (TIGR), at 584,848 to 585,366
DVU0511
DVU0512: flgF - flagellar basal-body rod protein, putative (TIGR), at 585,708 to 586,490
flgF
DVU0513: flgG - flagellar basal-body rod protein FlgG (TIGR), at 586,547 to 587,329
flgG
DVU0514: DVU0514 - FlgA family protein (TIGR), at 587,379 to 588,311
DVU0514
Position (kb) 585
586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 584.827 kb on + strand at 584.854 kb on - strand at 584.899 kb on - strand at 584.931 kb on - strand, within DVU0511 at 585.088 kb on + strand, within DVU0511 at 585.096 kb on - strand, within DVU0511 at 585.096 kb on - strand, within DVU0511 at 585.593 kb on - strand at 585.619 kb on - strand at 585.626 kb on + strand at 585.643 kb on + strand at 585.700 kb on - strand at 585.708 kb on + strand at 585.716 kb on - strand at 585.742 kb on - strand at 585.743 kb on + strand at 585.836 kb on + strand, within flgF at 585.838 kb on + strand, within flgF at 585.913 kb on - strand, within flgF at 585.920 kb on - strand, within flgF at 585.965 kb on + strand, within flgF at 586.029 kb on - strand, within flgF at 586.037 kb on - strand, within flgF at 586.097 kb on - strand, within flgF at 586.104 kb on + strand, within flgF at 586.163 kb on + strand, within flgF at 586.200 kb on - strand, within flgF at 586.207 kb on + strand, within flgF at 586.209 kb on - strand, within flgF at 586.209 kb on - strand, within flgF at 586.225 kb on - strand, within flgF at 586.269 kb on + strand, within flgF at 586.354 kb on + strand, within flgF at 586.390 kb on - strand, within flgF at 586.438 kb on + strand at 586.524 kb on + strand at 586.566 kb on - strand at 586.748 kb on - strand, within flgG at 586.765 kb on + strand, within flgG at 586.775 kb on - strand, within flgG at 586.783 kb on - strand, within flgG at 586.820 kb on + strand, within flgG at 586.872 kb on - strand, within flgG at 586.872 kb on - strand, within flgG at 586.915 kb on + strand, within flgG at 587.063 kb on + strand, within flgG at 587.068 kb on - strand, within flgG at 587.117 kb on - strand, within flgG at 587.211 kb on - strand, within flgG at 587.269 kb on + strand at 587.294 kb on - strand at 587.309 kb on + strand at 587.324 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with TEMED 20mM (N) remove 584,827 + -0.5 584,854 - +0.5 584,899 - -2.0 584,931 - DVU0511 0.16 +1.2 585,088 + DVU0511 0.46 -0.3 585,096 - DVU0511 0.48 +0.7 585,096 - DVU0511 0.48 -0.6 585,593 - +0.1 585,619 - +0.6 585,626 + -0.2 585,643 + -0.0 585,700 - +0.0 585,708 + -1.0 585,716 - -2.1 585,742 - -1.3 585,743 + -1.6 585,836 + flgF DVU0512 0.16 -1.5 585,838 + flgF DVU0512 0.17 -0.3 585,913 - flgF DVU0512 0.26 -0.7 585,920 - flgF DVU0512 0.27 -0.7 585,965 + flgF DVU0512 0.33 -1.5 586,029 - flgF DVU0512 0.41 -0.7 586,037 - flgF DVU0512 0.42 -1.7 586,097 - flgF DVU0512 0.50 -1.4 586,104 + flgF DVU0512 0.51 -1.2 586,163 + flgF DVU0512 0.58 -1.3 586,200 - flgF DVU0512 0.63 -1.7 586,207 + flgF DVU0512 0.64 -1.7 586,209 - flgF DVU0512 0.64 -3.0 586,209 - flgF DVU0512 0.64 -1.6 586,225 - flgF DVU0512 0.66 -0.5 586,269 + flgF DVU0512 0.72 -0.7 586,354 + flgF DVU0512 0.83 +0.3 586,390 - flgF DVU0512 0.87 -1.3 586,438 + -0.7 586,524 + -1.9 586,566 - -1.5 586,748 - flgG DVU0513 0.26 -1.5 586,765 + flgG DVU0513 0.28 -0.9 586,775 - flgG DVU0513 0.29 -1.8 586,783 - flgG DVU0513 0.30 -1.7 586,820 + flgG DVU0513 0.35 -1.4 586,872 - flgG DVU0513 0.42 -1.7 586,872 - flgG DVU0513 0.42 -1.9 586,915 + flgG DVU0513 0.47 -1.6 587,063 + flgG DVU0513 0.66 -1.9 587,068 - flgG DVU0513 0.67 -1.7 587,117 - flgG DVU0513 0.73 -1.7 587,211 - flgG DVU0513 0.85 -1.9 587,269 + -1.8 587,294 - -1.3 587,309 + -1.3 587,324 + -0.3
Or see this region's nucleotide sequence