Experiment: MoLS4 with TEMED 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ksgA and DVU1798 are separated by 91 nucleotides DVU1798 and fusA-2 are separated by 6 nucleotides fusA-2 and DORF13417 overlap by 8 nucleotides
DVU1797: ksgA - dimethyladenosine transferase (TIGR), at 1,858,413 to 1,859,213
ksgA
DVU1798: DVU1798 - conserved hypothetical protein (TIGR), at 1,859,305 to 1,859,820
DVU1798
DVU1799: fusA-2 - translation elongation factor G (TIGR), at 1,859,827 to 1,861,875
fusA-2
DORF13417: DORF13417 - SEED: FIG00603341: hypothetical protein, at 1,861,868 to 1,862,878
DORF13417
DORF13424: DORF13424 - no description, at 1,862,856 to 1,862,999
DORF13424
Position (kb) 1859
1860
1861
1862 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1859.067 kb on + strand, within ksgA at 1859.183 kb on + strand at 1859.186 kb on - strand at 1859.196 kb on - strand at 1859.211 kb on - strand at 1859.236 kb on + strand at 1859.244 kb on + strand at 1859.439 kb on - strand, within DVU1798 at 1859.499 kb on + strand, within DVU1798 at 1859.515 kb on + strand, within DVU1798 at 1859.515 kb on + strand, within DVU1798 at 1859.534 kb on + strand, within DVU1798 at 1859.555 kb on + strand, within DVU1798 at 1859.685 kb on - strand, within DVU1798 at 1859.685 kb on - strand, within DVU1798 at 1859.788 kb on + strand at 1859.815 kb on + strand at 1859.847 kb on - strand at 1859.890 kb on + strand at 1859.890 kb on + strand at 1860.058 kb on - strand, within fusA-2 at 1860.085 kb on + strand, within fusA-2 at 1860.097 kb on + strand, within fusA-2 at 1860.121 kb on + strand, within fusA-2 at 1860.236 kb on + strand, within fusA-2 at 1860.236 kb on + strand, within fusA-2 at 1860.257 kb on - strand, within fusA-2 at 1860.262 kb on - strand, within fusA-2 at 1860.316 kb on + strand, within fusA-2 at 1860.536 kb on - strand, within fusA-2 at 1860.546 kb on + strand, within fusA-2 at 1860.630 kb on - strand, within fusA-2 at 1860.635 kb on - strand, within fusA-2 at 1860.676 kb on + strand, within fusA-2 at 1860.678 kb on + strand, within fusA-2 at 1860.686 kb on - strand, within fusA-2 at 1860.995 kb on - strand, within fusA-2 at 1861.070 kb on - strand, within fusA-2 at 1861.072 kb on + strand, within fusA-2 at 1861.080 kb on - strand, within fusA-2 at 1861.080 kb on - strand, within fusA-2 at 1861.080 kb on - strand, within fusA-2 at 1861.101 kb on - strand, within fusA-2 at 1861.184 kb on + strand, within fusA-2 at 1861.283 kb on - strand, within fusA-2 at 1861.283 kb on - strand, within fusA-2 at 1861.288 kb on + strand, within fusA-2 at 1861.312 kb on + strand, within fusA-2 at 1861.332 kb on - strand, within fusA-2 at 1861.440 kb on + strand, within fusA-2 at 1861.549 kb on - strand, within fusA-2 at 1861.690 kb on + strand at 1861.925 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with TEMED 10mM (N) remove 1,859,067 + ksgA DVU1797 0.82 -0.6 1,859,183 + -0.7 1,859,186 - +0.9 1,859,196 - +0.4 1,859,211 - -0.6 1,859,236 + +0.4 1,859,244 + +0.8 1,859,439 - DVU1798 0.26 +0.5 1,859,499 + DVU1798 0.38 +0.2 1,859,515 + DVU1798 0.41 +0.0 1,859,515 + DVU1798 0.41 +1.2 1,859,534 + DVU1798 0.44 +0.1 1,859,555 + DVU1798 0.48 -0.8 1,859,685 - DVU1798 0.74 +0.9 1,859,685 - DVU1798 0.74 -1.0 1,859,788 + -2.5 1,859,815 + -0.2 1,859,847 - -0.8 1,859,890 + -0.9 1,859,890 + +0.3 1,860,058 - fusA-2 DVU1799 0.11 -0.9 1,860,085 + fusA-2 DVU1799 0.13 -2.3 1,860,097 + fusA-2 DVU1799 0.13 -0.9 1,860,121 + fusA-2 DVU1799 0.14 -0.7 1,860,236 + fusA-2 DVU1799 0.20 -1.7 1,860,236 + fusA-2 DVU1799 0.20 -1.1 1,860,257 - fusA-2 DVU1799 0.21 -1.2 1,860,262 - fusA-2 DVU1799 0.21 -1.0 1,860,316 + fusA-2 DVU1799 0.24 -0.4 1,860,536 - fusA-2 DVU1799 0.35 +1.3 1,860,546 + fusA-2 DVU1799 0.35 -0.5 1,860,630 - fusA-2 DVU1799 0.39 +0.9 1,860,635 - fusA-2 DVU1799 0.39 -0.5 1,860,676 + fusA-2 DVU1799 0.41 -1.2 1,860,678 + fusA-2 DVU1799 0.42 -1.1 1,860,686 - fusA-2 DVU1799 0.42 +0.3 1,860,995 - fusA-2 DVU1799 0.57 +0.3 1,861,070 - fusA-2 DVU1799 0.61 -0.4 1,861,072 + fusA-2 DVU1799 0.61 -0.7 1,861,080 - fusA-2 DVU1799 0.61 -1.0 1,861,080 - fusA-2 DVU1799 0.61 -0.9 1,861,080 - fusA-2 DVU1799 0.61 -0.3 1,861,101 - fusA-2 DVU1799 0.62 -1.1 1,861,184 + fusA-2 DVU1799 0.66 -0.4 1,861,283 - fusA-2 DVU1799 0.71 -1.0 1,861,283 - fusA-2 DVU1799 0.71 -2.9 1,861,288 + fusA-2 DVU1799 0.71 -1.2 1,861,312 + fusA-2 DVU1799 0.72 -2.5 1,861,332 - fusA-2 DVU1799 0.73 -0.9 1,861,440 + fusA-2 DVU1799 0.79 -0.7 1,861,549 - fusA-2 DVU1799 0.84 -1.3 1,861,690 + -0.6 1,861,925 - -1.1
Or see this region's nucleotide sequence