Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Phe-2 and tRNA-Thr-4 are separated by 21 nucleotides tRNA-Thr-4 and SO3374 are separated by 488 nucleotides SO3374 and SO3374.1 are separated by 58 nucleotides SO3374.1 and SO3376 are separated by 94 nucleotides SO3376 and SO3377 overlap by 13 nucleotides
SO_t081: tRNA-Phe-2 - tRNA-Phe (RefSeq), at 3,514,336 to 3,514,411
tRNA-Phe-2
SO_t082: tRNA-Thr-4 - tRNA-Thr (RefSeq), at 3,514,433 to 3,514,508
tRNA-Thr-4
SO3374: SO3374 - conserved hypothetical protein (NCBI ptt file), at 3,514,997 to 3,515,341
SO3374
SO3374.1: SO3374.1 - hypothetical protein (NCBI ptt file), at 3,515,400 to 3,515,609
SO3374.1
SO3376: SO3376 - conserved hypothetical protein (NCBI ptt file), at 3,515,704 to 3,516,255
SO3376
SO3377: SO3377 - conserved hypothetical protein (NCBI ptt file), at 3,516,243 to 3,516,791
SO3377
Position (kb)
3515
3516 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3514.573 kb on + strand at 3514.581 kb on + strand at 3514.589 kb on - strand at 3514.589 kb on - strand at 3514.758 kb on + strand at 3514.826 kb on + strand at 3514.826 kb on + strand at 3514.836 kb on + strand at 3514.837 kb on + strand at 3514.867 kb on + strand at 3514.883 kb on + strand at 3514.891 kb on - strand at 3514.908 kb on - strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.938 kb on + strand at 3514.940 kb on + strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.946 kb on - strand at 3514.987 kb on - strand at 3515.111 kb on + strand, within SO3374 at 3515.171 kb on - strand, within SO3374 at 3515.171 kb on - strand, within SO3374 at 3515.208 kb on - strand, within SO3374 at 3515.220 kb on - strand, within SO3374 at 3515.343 kb on - strand at 3515.344 kb on - strand at 3515.358 kb on + strand at 3515.358 kb on + strand at 3515.377 kb on + strand at 3515.485 kb on - strand, within SO3374.1 at 3515.625 kb on + strand at 3515.633 kb on - strand at 3515.673 kb on + strand at 3515.687 kb on + strand at 3515.687 kb on + strand at 3515.748 kb on + strand at 3515.853 kb on + strand, within SO3376 at 3515.854 kb on - strand, within SO3376 at 3515.874 kb on + strand, within SO3376 at 3515.874 kb on + strand, within SO3376 at 3515.879 kb on + strand, within SO3376 at 3515.971 kb on + strand, within SO3376 at 3516.010 kb on + strand, within SO3376 at 3516.017 kb on + strand, within SO3376 at 3516.166 kb on + strand, within SO3376 at 3516.305 kb on - strand, within SO3377 at 3516.355 kb on + strand, within SO3377 at 3516.363 kb on - strand, within SO3377 at 3516.376 kb on + strand, within SO3377 at 3516.384 kb on - strand, within SO3377 at 3516.407 kb on - strand, within SO3377 at 3516.415 kb on - strand, within SO3377 at 3516.436 kb on + strand, within SO3377 at 3516.439 kb on - strand, within SO3377 at 3516.495 kb on + strand, within SO3377 at 3516.498 kb on - strand, within SO3377 at 3516.589 kb on - strand, within SO3377
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 3,514,573 + -0.6 3,514,581 + +1.0 3,514,589 - -1.4 3,514,589 - +2.1 3,514,758 + -0.2 3,514,826 + -2.5 3,514,826 + -0.3 3,514,836 + -0.7 3,514,837 + +0.4 3,514,867 + -0.3 3,514,883 + +0.3 3,514,891 - -0.1 3,514,908 - -0.2 3,514,938 + +1.6 3,514,938 + +0.5 3,514,938 + -0.4 3,514,938 + +0.0 3,514,938 + +0.2 3,514,938 + +0.4 3,514,938 + -0.9 3,514,938 + -1.1 3,514,940 + +0.3 3,514,946 - -0.1 3,514,946 - -2.2 3,514,946 - +1.5 3,514,946 - +1.6 3,514,946 - +1.0 3,514,946 - -0.6 3,514,946 - -1.9 3,514,946 - +0.6 3,514,946 - -1.7 3,514,987 - -0.5 3,515,111 + SO3374 0.33 +1.9 3,515,171 - SO3374 0.50 +2.6 3,515,171 - SO3374 0.50 -2.4 3,515,208 - SO3374 0.61 -0.5 3,515,220 - SO3374 0.65 -0.2 3,515,343 - -0.5 3,515,344 - -0.4 3,515,358 + +1.0 3,515,358 + -0.2 3,515,377 + -2.3 3,515,485 - SO3374.1 0.40 -3.0 3,515,625 + +0.4 3,515,633 - +2.3 3,515,673 + -1.8 3,515,687 + +0.6 3,515,687 + +1.1 3,515,748 + -2.7 3,515,853 + SO3376 0.27 -0.3 3,515,854 - SO3376 0.27 -0.9 3,515,874 + SO3376 0.31 +0.9 3,515,874 + SO3376 0.31 +1.7 3,515,879 + SO3376 0.32 +1.2 3,515,971 + SO3376 0.48 +0.6 3,516,010 + SO3376 0.55 +0.9 3,516,017 + SO3376 0.57 +0.4 3,516,166 + SO3376 0.84 +1.1 3,516,305 - SO3377 0.11 -3.2 3,516,355 + SO3377 0.20 -2.1 3,516,363 - SO3377 0.22 -1.0 3,516,376 + SO3377 0.24 -0.4 3,516,384 - SO3377 0.26 +0.9 3,516,407 - SO3377 0.30 +1.1 3,516,415 - SO3377 0.31 +1.4 3,516,436 + SO3377 0.35 +0.4 3,516,439 - SO3377 0.36 +0.9 3,516,495 + SO3377 0.46 +0.6 3,516,498 - SO3377 0.46 +0.2 3,516,589 - SO3377 0.63 -0.5
Or see this region's nucleotide sequence