Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3253 and flgM are separated by 75 nucleotides flgM and flgN are separated by 9 nucleotides flgN and SO3256 are separated by 117 nucleotides SO3256 and SO3257 overlap by 4 nucleotides SO3257 and SO3258 are separated by 273 nucleotides
SO3253: SO3253 - flagellar basal-body P-ring formation protein FlgA, putative (NCBI ptt file), at 3,390,904 to 3,391,611
SO3253
SO3254: flgM - negative regulator of flagellin synthesis FlgM (NCBI ptt file), at 3,391,687 to 3,392,007
flgM
SO3255: flgN - flagellar biosynthetic protein FlgN (NCBI ptt file), at 3,392,017 to 3,392,442
flgN
SO3256: SO3256 - conserved hypothetical protein (NCBI ptt file), at 3,392,560 to 3,393,009
SO3256
SO3257: SO3257 - conserved hypothetical protein (NCBI ptt file), at 3,393,006 to 3,393,638
SO3257
SO3258: SO3258 - conserved hypothetical protein (NCBI ptt file), at 3,393,912 to 3,395,087
SO3258
Position (kb)
3392
3393
3394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3391.616 kb on + strand at 3391.667 kb on + strand at 3391.731 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.987 kb on + strand at 3392.042 kb on + strand at 3392.253 kb on + strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.336 kb on + strand, within flgN at 3392.341 kb on + strand, within flgN at 3392.349 kb on - strand, within flgN at 3392.394 kb on + strand, within flgN at 3392.529 kb on - strand at 3392.530 kb on + strand at 3392.540 kb on + strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.685 kb on + strand, within SO3256 at 3392.742 kb on + strand, within SO3256 at 3392.891 kb on - strand, within SO3256 at 3392.904 kb on + strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3393.089 kb on + strand, within SO3257 at 3393.101 kb on + strand, within SO3257 at 3393.160 kb on - strand, within SO3257 at 3393.331 kb on - strand, within SO3257 at 3393.636 kb on - strand at 3393.640 kb on + strand at 3393.648 kb on - strand at 3393.677 kb on - strand at 3393.717 kb on - strand at 3393.758 kb on - strand at 3393.788 kb on - strand at 3393.843 kb on + strand at 3393.861 kb on - strand at 3393.872 kb on - strand at 3393.949 kb on + strand at 3393.957 kb on - strand at 3393.959 kb on + strand at 3393.971 kb on + strand at 3393.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 3,391,616 + +0.7 3,391,667 + -1.2 3,391,731 + flgM SO3254 0.14 -1.3 3,391,745 + flgM SO3254 0.18 -3.1 3,391,745 + flgM SO3254 0.18 -2.5 3,391,745 + flgM SO3254 0.18 -1.7 3,391,745 + flgM SO3254 0.18 -2.9 3,391,745 + flgM SO3254 0.18 -1.9 3,391,745 + flgM SO3254 0.18 -1.5 3,391,745 + flgM SO3254 0.18 -2.8 3,391,745 + flgM SO3254 0.18 +1.2 3,391,745 + flgM SO3254 0.18 -2.3 3,391,745 + flgM SO3254 0.18 -4.1 3,391,845 + flgM SO3254 0.49 -0.8 3,391,845 + flgM SO3254 0.49 -1.5 3,391,987 + +0.8 3,392,042 + -0.9 3,392,253 + flgN SO3255 0.55 +1.0 3,392,266 - flgN SO3255 0.58 +1.5 3,392,266 - flgN SO3255 0.58 +0.6 3,392,336 + flgN SO3255 0.75 -0.1 3,392,341 + flgN SO3255 0.76 +1.2 3,392,349 - flgN SO3255 0.78 +2.3 3,392,394 + flgN SO3255 0.88 +0.4 3,392,529 - +0.8 3,392,530 + -0.3 3,392,540 + +0.3 3,392,548 - -0.1 3,392,548 - -0.1 3,392,548 - -1.1 3,392,685 + SO3256 0.28 -2.4 3,392,742 + SO3256 0.40 -1.3 3,392,891 - SO3256 0.74 -0.3 3,392,904 + SO3256 0.76 -0.5 3,392,912 - SO3256 0.78 -0.9 3,392,912 - SO3256 0.78 -1.8 3,392,912 - SO3256 0.78 -2.2 3,393,089 + SO3257 0.13 +0.4 3,393,101 + SO3257 0.15 -0.5 3,393,160 - SO3257 0.24 -1.9 3,393,331 - SO3257 0.51 -2.6 3,393,636 - +2.1 3,393,640 + -0.7 3,393,648 - -1.4 3,393,677 - +0.5 3,393,717 - +0.3 3,393,758 - +0.8 3,393,788 - -0.6 3,393,843 + -1.6 3,393,861 - -0.6 3,393,872 - -2.0 3,393,949 + -0.9 3,393,957 - -0.4 3,393,959 + -4.2 3,393,971 + -2.2 3,393,979 - -1.0
Or see this region's nucleotide sequence