Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheV-3 and SO3253 are separated by 205 nucleotides SO3253 and flgM are separated by 75 nucleotides flgM and flgN are separated by 9 nucleotides flgN and SO3256 are separated by 117 nucleotides
SO3252: cheV-3 - chemotaxis protein CheV (NCBI ptt file), at 3,389,778 to 3,390,698
cheV-3
SO3253: SO3253 - flagellar basal-body P-ring formation protein FlgA, putative (NCBI ptt file), at 3,390,904 to 3,391,611
SO3253
SO3254: flgM - negative regulator of flagellin synthesis FlgM (NCBI ptt file), at 3,391,687 to 3,392,007
flgM
SO3255: flgN - flagellar biosynthetic protein FlgN (NCBI ptt file), at 3,392,017 to 3,392,442
flgN
SO3256: SO3256 - conserved hypothetical protein (NCBI ptt file), at 3,392,560 to 3,393,009
SO3256
SO3257: SO3257 - conserved hypothetical protein (NCBI ptt file), at 3,393,006 to 3,393,638
SO3257
Position (kb)
3391
3392
3393 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3390.695 kb on + strand at 3390.695 kb on + strand at 3390.703 kb on - strand at 3390.705 kb on + strand at 3390.708 kb on + strand at 3390.710 kb on + strand at 3390.718 kb on + strand at 3390.844 kb on - strand at 3390.846 kb on + strand at 3390.864 kb on + strand at 3390.872 kb on - strand at 3390.882 kb on - strand at 3390.974 kb on + strand at 3390.974 kb on + strand at 3390.974 kb on + strand at 3390.976 kb on + strand, within SO3253 at 3390.984 kb on - strand, within SO3253 at 3390.994 kb on - strand, within SO3253 at 3390.996 kb on - strand, within SO3253 at 3391.084 kb on - strand, within SO3253 at 3391.121 kb on + strand, within SO3253 at 3391.155 kb on + strand, within SO3253 at 3391.155 kb on + strand, within SO3253 at 3391.163 kb on + strand, within SO3253 at 3391.196 kb on - strand, within SO3253 at 3391.199 kb on + strand, within SO3253 at 3391.202 kb on - strand, within SO3253 at 3391.202 kb on - strand, within SO3253 at 3391.258 kb on + strand, within SO3253 at 3391.258 kb on + strand, within SO3253 at 3391.263 kb on + strand, within SO3253 at 3391.264 kb on - strand, within SO3253 at 3391.265 kb on + strand, within SO3253 at 3391.265 kb on + strand, within SO3253 at 3391.273 kb on - strand, within SO3253 at 3391.273 kb on - strand, within SO3253 at 3391.289 kb on + strand, within SO3253 at 3391.289 kb on + strand, within SO3253 at 3391.308 kb on - strand, within SO3253 at 3391.337 kb on + strand, within SO3253 at 3391.362 kb on + strand, within SO3253 at 3391.362 kb on + strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.383 kb on + strand, within SO3253 at 3391.391 kb on - strand, within SO3253 at 3391.422 kb on + strand, within SO3253 at 3391.496 kb on + strand, within SO3253 at 3391.510 kb on - strand, within SO3253 at 3391.616 kb on + strand at 3391.667 kb on + strand at 3391.731 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.987 kb on + strand at 3392.042 kb on + strand at 3392.253 kb on + strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.336 kb on + strand, within flgN at 3392.341 kb on + strand, within flgN at 3392.349 kb on - strand, within flgN at 3392.394 kb on + strand, within flgN at 3392.529 kb on - strand at 3392.530 kb on + strand at 3392.540 kb on + strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.685 kb on + strand, within SO3256 at 3392.742 kb on + strand, within SO3256 at 3392.891 kb on - strand, within SO3256 at 3392.904 kb on + strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 3,390,695 + -1.9 3,390,695 + +1.4 3,390,703 - -0.1 3,390,705 + -0.5 3,390,708 + +0.6 3,390,710 + +0.5 3,390,718 + -1.8 3,390,844 - -1.9 3,390,846 + +0.3 3,390,864 + +1.2 3,390,872 - +0.3 3,390,882 - -2.5 3,390,974 + -0.9 3,390,974 + +0.2 3,390,974 + -1.6 3,390,976 + SO3253 0.10 -1.7 3,390,984 - SO3253 0.11 -2.0 3,390,994 - SO3253 0.13 -1.9 3,390,996 - SO3253 0.13 +0.7 3,391,084 - SO3253 0.25 -3.1 3,391,121 + SO3253 0.31 -2.1 3,391,155 + SO3253 0.35 -1.7 3,391,155 + SO3253 0.35 -2.9 3,391,163 + SO3253 0.37 +0.5 3,391,196 - SO3253 0.41 +0.4 3,391,199 + SO3253 0.42 -0.4 3,391,202 - SO3253 0.42 -2.1 3,391,202 - SO3253 0.42 -1.9 3,391,258 + SO3253 0.50 +0.2 3,391,258 + SO3253 0.50 -0.5 3,391,263 + SO3253 0.51 -0.3 3,391,264 - SO3253 0.51 -0.2 3,391,265 + SO3253 0.51 +0.1 3,391,265 + SO3253 0.51 -1.8 3,391,273 - SO3253 0.52 -2.3 3,391,273 - SO3253 0.52 -0.3 3,391,289 + SO3253 0.54 -2.2 3,391,289 + SO3253 0.54 -0.6 3,391,308 - SO3253 0.57 -1.3 3,391,337 + SO3253 0.61 +0.2 3,391,362 + SO3253 0.65 -1.5 3,391,362 + SO3253 0.65 +0.1 3,391,370 - SO3253 0.66 -1.2 3,391,370 - SO3253 0.66 -2.0 3,391,370 - SO3253 0.66 +0.8 3,391,370 - SO3253 0.66 +0.3 3,391,383 + SO3253 0.68 +0.3 3,391,391 - SO3253 0.69 -1.1 3,391,422 + SO3253 0.73 -3.5 3,391,496 + SO3253 0.84 -1.1 3,391,510 - SO3253 0.86 +0.4 3,391,616 + +0.7 3,391,667 + -1.2 3,391,731 + flgM SO3254 0.14 -1.3 3,391,745 + flgM SO3254 0.18 -3.1 3,391,745 + flgM SO3254 0.18 -2.5 3,391,745 + flgM SO3254 0.18 -1.7 3,391,745 + flgM SO3254 0.18 -2.9 3,391,745 + flgM SO3254 0.18 -1.9 3,391,745 + flgM SO3254 0.18 -1.5 3,391,745 + flgM SO3254 0.18 -2.8 3,391,745 + flgM SO3254 0.18 +1.2 3,391,745 + flgM SO3254 0.18 -2.3 3,391,745 + flgM SO3254 0.18 -4.1 3,391,845 + flgM SO3254 0.49 -0.8 3,391,845 + flgM SO3254 0.49 -1.5 3,391,987 + +0.8 3,392,042 + -0.9 3,392,253 + flgN SO3255 0.55 +1.0 3,392,266 - flgN SO3255 0.58 +1.5 3,392,266 - flgN SO3255 0.58 +0.6 3,392,336 + flgN SO3255 0.75 -0.1 3,392,341 + flgN SO3255 0.76 +1.2 3,392,349 - flgN SO3255 0.78 +2.3 3,392,394 + flgN SO3255 0.88 +0.4 3,392,529 - +0.8 3,392,530 + -0.3 3,392,540 + +0.3 3,392,548 - -0.1 3,392,548 - -0.1 3,392,548 - -1.1 3,392,685 + SO3256 0.28 -2.4 3,392,742 + SO3256 0.40 -1.3 3,392,891 - SO3256 0.74 -0.3 3,392,904 + SO3256 0.76 -0.5 3,392,912 - SO3256 0.78 -0.9 3,392,912 - SO3256 0.78 -1.8 3,392,912 - SO3256 0.78 -2.2
Or see this region's nucleotide sequence