Strain Fitness in Shewanella oneidensis MR-1 around SO3224

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliK and fliJ are separated by 143 nucleotidesfliJ and fliI are separated by 11 nucleotides SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689 fliK SO3224: fliJ - flagellar protein FliJ (NCBI ptt file), at 3,362,833 to 3,363,276 fliJ SO3225: fliI - flagellum-specific ATP synthase FliI (NCBI ptt file), at 3,363,288 to 3,364,625 fliI Position (kb) 3362 3363 3364Strain fitness (log2 ratio) -3 -2 -1 0 1at 3361.918 kb on + strand, within fliKat 3362.053 kb on + strand, within fliKat 3362.086 kb on + strand, within fliKat 3362.094 kb on - strand, within fliKat 3362.103 kb on + strand, within fliKat 3362.244 kb on - strand, within fliKat 3362.248 kb on + strand, within fliKat 3362.263 kb on - strand, within fliKat 3362.496 kb on - strand, within fliKat 3362.695 kb on + strandat 3362.980 kb on - strand, within fliJat 3363.084 kb on + strand, within fliJat 3363.092 kb on + strand, within fliJat 3363.100 kb on - strand, within fliJat 3363.100 kb on - strand, within fliJat 3363.338 kb on + strandat 3363.502 kb on + strand, within fliIat 3363.502 kb on + strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.608 kb on + strand, within fliIat 3363.613 kb on - strand, within fliIat 3363.690 kb on - strand, within fliIat 3363.707 kb on + strand, within fliIat 3363.707 kb on + strand, within fliIat 3363.935 kb on - strand, within fliIat 3363.935 kb on - strand, within fliIat 3364.071 kb on - strand, within fliIat 3364.096 kb on + strand, within fliIat 3364.235 kb on + strand, within fliI

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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3,361,918 + fliK SO3223 0.51 -2.2
3,362,053 + fliK SO3223 0.60 -2.8
3,362,086 + fliK SO3223 0.62 +0.6
3,362,094 - fliK SO3223 0.62 -0.7
3,362,103 + fliK SO3223 0.63 +0.7
3,362,244 - fliK SO3223 0.72 -0.3
3,362,248 + fliK SO3223 0.72 +1.3
3,362,263 - fliK SO3223 0.73 -0.3
3,362,496 - fliK SO3223 0.88 +0.0
3,362,695 + -0.4
3,362,980 - fliJ SO3224 0.33 -3.7
3,363,084 + fliJ SO3224 0.57 -0.5
3,363,092 + fliJ SO3224 0.58 -0.9
3,363,100 - fliJ SO3224 0.60 -2.6
3,363,100 - fliJ SO3224 0.60 -1.9
3,363,338 + -1.4
3,363,502 + fliI SO3225 0.16 -1.3
3,363,502 + fliI SO3225 0.16 -1.8
3,363,510 - fliI SO3225 0.17 -1.0
3,363,510 - fliI SO3225 0.17 -0.3
3,363,510 - fliI SO3225 0.17 -0.7
3,363,510 - fliI SO3225 0.17 +0.3
3,363,608 + fliI SO3225 0.24 +0.0
3,363,613 - fliI SO3225 0.24 +0.7
3,363,690 - fliI SO3225 0.30 -3.7
3,363,707 + fliI SO3225 0.31 -1.7
3,363,707 + fliI SO3225 0.31 -2.4
3,363,935 - fliI SO3225 0.48 -2.5
3,363,935 - fliI SO3225 0.48 -0.7
3,364,071 - fliI SO3225 0.59 -0.1
3,364,096 + fliI SO3225 0.60 -0.9
3,364,235 + fliI SO3225 0.71 -2.1

Or see this region's nucleotide sequence