Strain Fitness in Shewanella oneidensis MR-1 around SO3210

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcheZ and cheY-3 are separated by 13 nucleotidescheY-3 and fliA are separated by 47 nucleotidesfliA and flhG overlap by 8 nucleotidesflhG and flhF are separated by 34 nucleotides SO3208: cheZ - chemotaxis protein CheZ (NCBI ptt file), at 3,348,973 to 3,349,710 cheZ SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107 cheY-3 SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868 fliA SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712 flhG SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123 flhF Position (kb) 3350 3351Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3349.302 kb on + strand, within cheZat 3349.656 kb on - strandat 3349.666 kb on - strandat 3349.689 kb on + strandat 3349.712 kb on + strandat 3349.713 kb on - strandat 3349.720 kb on - strandat 3349.767 kb on - strand, within cheY-3at 3349.789 kb on + strand, within cheY-3at 3349.899 kb on + strand, within cheY-3at 3350.007 kb on + strand, within cheY-3at 3350.116 kb on - strandat 3350.191 kb on - strandat 3350.534 kb on - strand, within fliAat 3350.671 kb on - strand, within fliAat 3350.686 kb on - strand, within fliAat 3350.731 kb on - strand, within fliAat 3350.732 kb on + strand, within fliAat 3350.845 kb on + strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3350.853 kb on - strandat 3351.311 kb on - strand, within flhGat 3351.457 kb on - strand, within flhGat 3351.511 kb on - strand, within flhGat 3351.571 kb on - strand, within flhGat 3351.591 kb on + strand, within flhGat 3351.638 kb on + strandat 3351.646 kb on - strandat 3351.770 kb on - strandat 3351.770 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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3,349,302 + cheZ SO3208 0.45 -2.7
3,349,656 - +0.2
3,349,666 - -0.6
3,349,689 + +0.4
3,349,712 + -0.7
3,349,713 - +0.4
3,349,720 - -2.0
3,349,767 - cheY-3 SO3209 0.11 -0.1
3,349,789 + cheY-3 SO3209 0.17 -2.3
3,349,899 + cheY-3 SO3209 0.46 -0.4
3,350,007 + cheY-3 SO3209 0.74 -1.3
3,350,116 - -1.0
3,350,191 - -0.8
3,350,534 - fliA SO3210 0.53 -3.7
3,350,671 - fliA SO3210 0.72 -1.9
3,350,686 - fliA SO3210 0.74 -2.0
3,350,731 - fliA SO3210 0.81 -0.3
3,350,732 + fliA SO3210 0.81 -0.6
3,350,845 + -2.4
3,350,853 - -2.2
3,350,853 - -2.8
3,350,853 - -2.1
3,351,311 - flhG SO3211 0.53 -3.1
3,351,457 - flhG SO3211 0.70 -3.0
3,351,511 - flhG SO3211 0.76 -0.3
3,351,571 - flhG SO3211 0.83 -2.4
3,351,591 + flhG SO3211 0.86 -0.1
3,351,638 + -0.1
3,351,646 - -1.9
3,351,770 - +0.4
3,351,770 - +1.9

Or see this region's nucleotide sequence