Strain Fitness in Shewanella oneidensis MR-1 around SO2999

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2995 and SO2997 are separated by 168 nucleotidesSO2997 and SO2998 overlap by 8 nucleotidesSO2998 and SO2999 are separated by 83 nucleotidesSO2999 and SO3000 are separated by 9 nucleotidesSO3000 and SO3001 overlap by 4 nucleotidesSO3001 and SO3002 overlap by 4 nucleotides SO2995: SO2995 - hypothetical protein (NCBI ptt file), at 3,117,005 to 3,117,241 SO2995 SO2997: SO2997 - hypothetical protein (NCBI ptt file), at 3,117,410 to 3,117,715 SO2997 SO2998: SO2998 - hypothetical protein (NCBI ptt file), at 3,117,708 to 3,117,989 SO2998 SO2999: SO2999 - hypothetical protein (NCBI ptt file), at 3,118,073 to 3,118,237 SO2999 SO3000: SO3000 - conserved hypothetical protein (NCBI ptt file), at 3,118,247 to 3,118,834 SO3000 SO3001: SO3001 - hypothetical protein (NCBI ptt file), at 3,118,831 to 3,119,220 SO3001 SO3002: SO3002 - conserved hypothetical protein (NCBI ptt file), at 3,119,217 to 3,119,888 SO3002 Position (kb) 3118 3119Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3117.162 kb on - strand, within SO2995at 3117.276 kb on - strandat 3117.277 kb on - strandat 3117.277 kb on - strandat 3117.279 kb on + strandat 3117.282 kb on - strandat 3117.299 kb on + strandat 3117.320 kb on + strandat 3117.320 kb on + strandat 3117.320 kb on + strandat 3117.328 kb on - strandat 3117.378 kb on - strandat 3117.425 kb on - strandat 3117.465 kb on + strand, within SO2997at 3117.470 kb on + strand, within SO2997at 3117.483 kb on + strand, within SO2997at 3117.504 kb on + strand, within SO2997at 3117.514 kb on + strand, within SO2997at 3117.672 kb on + strand, within SO2997at 3117.697 kb on + strandat 3117.794 kb on - strand, within SO2998at 3117.864 kb on - strand, within SO2998at 3118.082 kb on - strandat 3118.091 kb on + strand, within SO2999at 3118.091 kb on + strand, within SO2999at 3118.099 kb on - strand, within SO2999at 3118.241 kb on + strandat 3118.440 kb on - strand, within SO3000at 3118.455 kb on + strand, within SO3000at 3118.576 kb on - strand, within SO3000at 3118.667 kb on - strand, within SO3000at 3118.738 kb on + strand, within SO3000at 3118.738 kb on + strand, within SO3000at 3118.738 kb on + strand, within SO3000at 3118.738 kb on + strand, within SO3000at 3118.739 kb on + strand, within SO3000at 3118.746 kb on - strand, within SO3000at 3118.747 kb on - strand, within SO3000at 3118.773 kb on - strand, within SO3000at 3118.792 kb on + strandat 3118.810 kb on + strandat 3118.880 kb on - strand, within SO3001at 3118.963 kb on + strand, within SO3001at 3118.971 kb on - strand, within SO3001at 3118.975 kb on + strand, within SO3001at 3118.983 kb on + strand, within SO3001at 3118.996 kb on + strand, within SO3001at 3119.132 kb on - strand, within SO3001at 3119.141 kb on + strand, within SO3001at 3119.170 kb on + strand, within SO3001at 3119.201 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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3,117,162 - SO2995 0.66 +1.4
3,117,276 - +1.8
3,117,277 - -1.3
3,117,277 - +0.4
3,117,279 + +0.6
3,117,282 - -1.6
3,117,299 + -0.2
3,117,320 + -1.3
3,117,320 + +0.8
3,117,320 + +0.6
3,117,328 - -0.6
3,117,378 - +1.0
3,117,425 - -0.9
3,117,465 + SO2997 0.18 -2.3
3,117,470 + SO2997 0.20 -2.3
3,117,483 + SO2997 0.24 -0.8
3,117,504 + SO2997 0.31 -3.0
3,117,514 + SO2997 0.34 -0.5
3,117,672 + SO2997 0.86 -1.4
3,117,697 + -0.7
3,117,794 - SO2998 0.30 +0.7
3,117,864 - SO2998 0.55 +0.1
3,118,082 - -0.8
3,118,091 + SO2999 0.11 -2.8
3,118,091 + SO2999 0.11 -0.6
3,118,099 - SO2999 0.16 -1.2
3,118,241 + -2.3
3,118,440 - SO3000 0.33 -0.1
3,118,455 + SO3000 0.35 +0.4
3,118,576 - SO3000 0.56 -3.5
3,118,667 - SO3000 0.71 -0.4
3,118,738 + SO3000 0.84 +0.9
3,118,738 + SO3000 0.84 -0.4
3,118,738 + SO3000 0.84 -1.7
3,118,738 + SO3000 0.84 +0.4
3,118,739 + SO3000 0.84 +0.0
3,118,746 - SO3000 0.85 -0.7
3,118,747 - SO3000 0.85 +1.2
3,118,773 - SO3000 0.89 -2.6
3,118,792 + -0.6
3,118,810 + -1.1
3,118,880 - SO3001 0.13 +1.2
3,118,963 + SO3001 0.34 -1.2
3,118,971 - SO3001 0.36 -0.6
3,118,975 + SO3001 0.37 +3.1
3,118,983 + SO3001 0.39 -0.3
3,118,996 + SO3001 0.42 +0.2
3,119,132 - SO3001 0.77 -1.7
3,119,141 + SO3001 0.79 -1.1
3,119,170 + SO3001 0.87 -0.4
3,119,201 + +1.0

Or see this region's nucleotide sequence