Strain Fitness in Shewanella oneidensis MR-1 around SO2277

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2275 and SO2276 overlap by 11 nucleotidesSO2276 and ibpA are separated by 214 nucleotidesibpA and ilvH are separated by 107 nucleotidesilvH and ilvI overlap by 1 nucleotides SO2275: SO2275 - ISSod10, transposase OrfA (NCBI ptt file), at 2,387,572 to 2,388,051 SO2275 SO2276: SO2276 - ISSod10, transposase OrfB (NCBI ptt file), at 2,388,041 to 2,388,598 SO2276 SO2277: ibpA - 16 kDa heat shock protein A (NCBI ptt file), at 2,388,813 to 2,389,256 ibpA SO2278: ilvH - acetolactate synthase III, small subunit (NCBI ptt file), at 2,389,364 to 2,389,858 ilvH SO2279: ilvI - acetolactate synthase III, large subunit (NCBI ptt file), at 2,389,858 to 2,391,576 ilvI Position (kb) 2388 2389 2390Strain fitness (log2 ratio) -3 -2 -1 0 1at 2388.803 kb on + strandat 2388.804 kb on + strandat 2388.865 kb on + strand, within ibpAat 2389.028 kb on + strand, within ibpAat 2389.059 kb on - strand, within ibpAat 2389.104 kb on - strand, within ibpAat 2389.108 kb on + strand, within ibpAat 2389.245 kb on + strandat 2389.275 kb on + strandat 2389.399 kb on - strandat 2389.412 kb on - strandat 2389.587 kb on + strand, within ilvHat 2389.864 kb on - strandat 2390.116 kb on - strand, within ilvIat 2390.116 kb on - strand, within ilvIat 2390.121 kb on - strand, within ilvIat 2390.185 kb on + strand, within ilvI

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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2,388,803 + -0.3
2,388,804 + -0.6
2,388,865 + ibpA SO2277 0.12 -2.8
2,389,028 + ibpA SO2277 0.48 -0.4
2,389,059 - ibpA SO2277 0.55 +0.4
2,389,104 - ibpA SO2277 0.66 -3.0
2,389,108 + ibpA SO2277 0.66 -0.7
2,389,245 + -0.1
2,389,275 + +1.7
2,389,399 - -1.7
2,389,412 - -0.8
2,389,587 + ilvH SO2278 0.45 -0.8
2,389,864 - +0.7
2,390,116 - ilvI SO2279 0.15 -2.2
2,390,116 - ilvI SO2279 0.15 -1.6
2,390,121 - ilvI SO2279 0.15 -1.3
2,390,185 + ilvI SO2279 0.19 -1.2

Or see this region's nucleotide sequence