Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0525 and SO0526 are separated by 138 nucleotides SO0526 and SO0527 are separated by 43 nucleotides SO0527 and SO0528 are separated by 26 nucleotides SO0528 and trpI are separated by 59 nucleotides
SO0525: SO0525 - drug resistance transporter, EmrB/QacA family protein (NCBI ptt file), at 548,245 to 549,864
SO0525
SO0526: SO0526 - acetyltransferase, GNAT family (NCBI ptt file), at 550,003 to 550,476
SO0526
SO0527: SO0527 - conserved hypothetical protein (NCBI ptt file), at 550,520 to 550,846
SO0527
SO0528: SO0528 - conserved hypothetical protein (NCBI ptt file), at 550,873 to 551,760
SO0528
SO0529: trpI - trpba operon transcriptional activator (NCBI ptt file), at 551,820 to 552,704
trpI
Position (kb) 550
551 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 549.583 kb on + strand, within SO0525 at 549.583 kb on + strand, within SO0525 at 549.591 kb on - strand, within SO0525 at 549.603 kb on + strand, within SO0525 at 549.638 kb on + strand, within SO0525 at 549.646 kb on - strand, within SO0525 at 549.646 kb on - strand, within SO0525 at 549.681 kb on + strand, within SO0525 at 549.702 kb on + strand, within SO0525 at 549.710 kb on - strand at 549.746 kb on - strand at 549.826 kb on + strand at 549.826 kb on + strand at 549.826 kb on + strand at 549.826 kb on + strand at 549.834 kb on - strand at 549.834 kb on - strand at 549.834 kb on - strand at 549.865 kb on + strand at 549.895 kb on + strand at 549.949 kb on + strand at 549.959 kb on + strand at 549.967 kb on - strand at 549.981 kb on - strand at 550.108 kb on + strand, within SO0526 at 550.113 kb on + strand, within SO0526 at 550.116 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.238 kb on - strand, within SO0526 at 550.243 kb on - strand, within SO0526 at 550.263 kb on + strand, within SO0526 at 550.301 kb on + strand, within SO0526 at 550.309 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.430 kb on - strand at 550.456 kb on - strand at 550.461 kb on + strand at 550.664 kb on - strand, within SO0527 at 550.737 kb on + strand, within SO0527 at 550.745 kb on - strand, within SO0527 at 550.933 kb on - strand at 550.941 kb on - strand at 551.030 kb on - strand, within SO0528 at 551.106 kb on + strand, within SO0528 at 551.107 kb on + strand, within SO0528 at 551.175 kb on + strand, within SO0528 at 551.183 kb on - strand, within SO0528 at 551.249 kb on + strand, within SO0528 at 551.259 kb on + strand, within SO0528 at 551.286 kb on - strand, within SO0528 at 551.395 kb on + strand, within SO0528 at 551.395 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.512 kb on - strand, within SO0528 at 551.522 kb on - strand, within SO0528 at 551.568 kb on - strand, within SO0528 at 551.597 kb on - strand, within SO0528 at 551.602 kb on - strand, within SO0528 at 551.659 kb on + strand, within SO0528 at 551.670 kb on + strand, within SO0528 at 551.670 kb on + strand, within SO0528 at 551.678 kb on - strand at 551.711 kb on - strand at 551.725 kb on + strand at 551.731 kb on - strand at 551.763 kb on + strand at 551.783 kb on + strand at 551.845 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 549,583 + SO0525 0.83 +0.4 549,583 + SO0525 0.83 +1.0 549,591 - SO0525 0.83 -2.6 549,603 + SO0525 0.84 +0.0 549,638 + SO0525 0.86 +0.0 549,646 - SO0525 0.86 +0.3 549,646 - SO0525 0.86 +0.2 549,681 + SO0525 0.89 -0.0 549,702 + SO0525 0.90 -0.7 549,710 - +0.2 549,746 - +0.4 549,826 + +0.9 549,826 + -0.9 549,826 + +1.9 549,826 + -0.5 549,834 - +0.1 549,834 - -1.1 549,834 - -0.7 549,865 + +0.5 549,895 + -0.4 549,949 + -1.2 549,959 + -0.9 549,967 - -0.3 549,981 - -3.2 550,108 + SO0526 0.22 -0.5 550,113 + SO0526 0.23 -0.8 550,116 - SO0526 0.24 +0.1 550,121 - SO0526 0.25 +0.1 550,121 - SO0526 0.25 -1.1 550,121 - SO0526 0.25 +1.2 550,238 - SO0526 0.50 +0.0 550,243 - SO0526 0.51 -0.4 550,263 + SO0526 0.55 -1.2 550,301 + SO0526 0.63 -1.5 550,309 - SO0526 0.65 -0.8 550,423 - SO0526 0.89 +0.3 550,423 - SO0526 0.89 +3.5 550,423 - SO0526 0.89 +0.3 550,430 - -1.6 550,456 - +2.2 550,461 + +0.5 550,664 - SO0527 0.44 -2.2 550,737 + SO0527 0.66 -0.8 550,745 - SO0527 0.69 -1.5 550,933 - +0.2 550,941 - -1.0 551,030 - SO0528 0.18 -1.7 551,106 + SO0528 0.26 -0.7 551,107 + SO0528 0.26 +0.6 551,175 + SO0528 0.34 -1.0 551,183 - SO0528 0.35 -0.8 551,249 + SO0528 0.42 -2.5 551,259 + SO0528 0.43 +0.6 551,286 - SO0528 0.47 +0.5 551,395 + SO0528 0.59 -0.4 551,395 + SO0528 0.59 +0.3 551,504 + SO0528 0.71 -0.8 551,504 + SO0528 0.71 +0.8 551,504 + SO0528 0.71 +3.0 551,512 - SO0528 0.72 -0.1 551,522 - SO0528 0.73 +0.6 551,568 - SO0528 0.78 -1.1 551,597 - SO0528 0.82 -0.5 551,602 - SO0528 0.82 -1.0 551,659 + SO0528 0.89 +0.2 551,670 + SO0528 0.90 +2.1 551,670 + SO0528 0.90 -0.2 551,678 - +0.6 551,711 - +0.2 551,725 + -0.2 551,731 - +1.8 551,763 + +0.1 551,783 + +2.4 551,845 + +0.6
Or see this region's nucleotide sequence