Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspD and gspE overlap by 4 nucleotides gspE and gspF are separated by 3 nucleotides
SO0166: gspD - general secretion pathway protein D (NCBI ptt file), at 172,301 to 174,415
gspD
SO0167: gspE - general secretion pathway protein E (NCBI ptt file), at 174,412 to 175,977
gspE
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
Position (kb) 174
175
176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 173.416 kb on - strand, within gspD at 173.434 kb on - strand, within gspD at 173.500 kb on + strand, within gspD at 173.500 kb on + strand, within gspD at 173.588 kb on + strand, within gspD at 173.606 kb on + strand, within gspD at 173.679 kb on + strand, within gspD at 173.705 kb on - strand, within gspD at 173.781 kb on + strand, within gspD at 173.808 kb on + strand, within gspD at 173.836 kb on + strand, within gspD at 173.871 kb on + strand, within gspD at 174.092 kb on + strand, within gspD at 174.220 kb on + strand at 174.271 kb on + strand at 174.271 kb on + strand at 174.275 kb on - strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.352 kb on + strand at 174.360 kb on - strand at 174.588 kb on + strand, within gspE at 174.646 kb on - strand, within gspE at 174.654 kb on + strand, within gspE at 174.669 kb on - strand, within gspE at 174.702 kb on + strand, within gspE at 174.733 kb on - strand, within gspE at 174.797 kb on + strand, within gspE at 174.807 kb on + strand, within gspE at 174.815 kb on - strand, within gspE at 174.833 kb on + strand, within gspE at 174.843 kb on + strand, within gspE at 174.866 kb on - strand, within gspE at 174.953 kb on - strand, within gspE at 174.976 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 175.007 kb on - strand, within gspE at 175.013 kb on - strand, within gspE at 175.269 kb on + strand, within gspE at 175.285 kb on - strand, within gspE at 175.407 kb on - strand, within gspE at 175.451 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.550 kb on - strand, within gspE at 175.570 kb on + strand, within gspE at 175.578 kb on + strand, within gspE at 175.710 kb on + strand, within gspE at 175.817 kb on + strand, within gspE at 175.951 kb on + strand at 175.959 kb on - strand at 175.972 kb on + strand at 175.979 kb on - strand at 175.980 kb on - strand at 175.980 kb on - strand at 176.005 kb on - strand at 176.010 kb on + strand at 176.025 kb on + strand at 176.033 kb on - strand at 176.033 kb on - strand at 176.134 kb on + strand, within gspF at 176.229 kb on - strand, within gspF at 176.247 kb on + strand, within gspF at 176.247 kb on + strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.260 kb on + strand, within gspF at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 173,416 - gspD SO0166 0.53 -3.3 173,434 - gspD SO0166 0.54 -3.1 173,500 + gspD SO0166 0.57 +1.1 173,500 + gspD SO0166 0.57 -2.0 173,588 + gspD SO0166 0.61 -0.2 173,606 + gspD SO0166 0.62 +1.7 173,679 + gspD SO0166 0.65 -1.8 173,705 - gspD SO0166 0.66 -1.7 173,781 + gspD SO0166 0.70 +1.8 173,808 + gspD SO0166 0.71 -2.9 173,836 + gspD SO0166 0.73 -2.0 173,871 + gspD SO0166 0.74 -2.4 174,092 + gspD SO0166 0.85 -2.0 174,220 + -0.9 174,271 + -0.4 174,271 + -0.2 174,275 - -1.5 174,335 + -1.2 174,335 + -1.8 174,335 + -2.6 174,335 + -2.8 174,352 + +2.0 174,360 - -2.0 174,588 + gspE SO0167 0.11 -3.7 174,646 - gspE SO0167 0.15 -3.5 174,654 + gspE SO0167 0.15 +1.1 174,669 - gspE SO0167 0.16 -1.7 174,702 + gspE SO0167 0.19 -1.5 174,733 - gspE SO0167 0.20 -2.0 174,797 + gspE SO0167 0.25 -1.6 174,807 + gspE SO0167 0.25 -3.7 174,815 - gspE SO0167 0.26 -0.5 174,833 + gspE SO0167 0.27 -1.5 174,843 + gspE SO0167 0.28 -3.0 174,866 - gspE SO0167 0.29 -3.7 174,953 - gspE SO0167 0.35 -0.7 174,976 + gspE SO0167 0.36 -3.4 174,999 + gspE SO0167 0.37 -1.7 174,999 + gspE SO0167 0.37 -2.0 174,999 + gspE SO0167 0.37 -2.4 175,007 - gspE SO0167 0.38 -1.3 175,013 - gspE SO0167 0.38 -1.9 175,269 + gspE SO0167 0.55 -3.3 175,285 - gspE SO0167 0.56 -3.4 175,407 - gspE SO0167 0.64 -1.2 175,451 - gspE SO0167 0.66 -3.1 175,484 - gspE SO0167 0.68 -1.8 175,484 - gspE SO0167 0.68 -4.1 175,550 - gspE SO0167 0.73 -0.7 175,570 + gspE SO0167 0.74 -3.2 175,578 + gspE SO0167 0.74 -3.5 175,710 + gspE SO0167 0.83 -1.3 175,817 + gspE SO0167 0.90 -1.0 175,951 + -0.6 175,959 - -2.0 175,972 + -0.5 175,979 - -0.7 175,980 - -3.0 175,980 - -2.6 176,005 - -3.5 176,010 + -2.0 176,025 + -2.0 176,033 - -2.0 176,033 - +0.1 176,134 + gspF SO0168 0.12 -0.2 176,229 - gspF SO0168 0.20 -1.8 176,247 + gspF SO0168 0.22 -2.2 176,247 + gspF SO0168 0.22 -0.9 176,255 - gspF SO0168 0.22 -2.8 176,255 - gspF SO0168 0.22 -1.5 176,255 - gspF SO0168 0.22 -0.4 176,255 - gspF SO0168 0.22 -1.6 176,255 - gspF SO0168 0.22 -2.3 176,260 + gspF SO0168 0.23 +0.5 176,289 - gspF SO0168 0.25 -3.8 176,327 + gspF SO0168 0.28 -2.3 176,327 + gspF SO0168 0.28 -4.2 176,335 - gspF SO0168 0.29 -3.2 176,382 - gspF SO0168 0.33 -0.7 176,382 - gspF SO0168 0.33 +0.5 176,524 - gspF SO0168 0.44 -3.0 176,555 - gspF SO0168 0.47 +0.1 176,597 - gspF SO0168 0.50 -1.2 176,640 - gspF SO0168 0.54 -1.3 176,795 - gspF SO0168 0.67 -2.3 176,834 + gspF SO0168 0.70 -0.9 176,834 + gspF SO0168 0.70 -1.3 176,936 + gspF SO0168 0.78 -1.9 176,941 + gspF SO0168 0.78 +1.2 176,941 + gspF SO0168 0.78 -1.7 176,941 + gspF SO0168 0.78 -0.5 176,953 + gspF SO0168 0.79 -2.5 176,958 + gspF SO0168 0.80 +2.1 176,958 + gspF SO0168 0.80 -2.5 176,966 - gspF SO0168 0.80 -2.2 176,966 - gspF SO0168 0.80 -0.5
Or see this region's nucleotide sequence