Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspC and gspD are separated by 24 nucleotides gspD and gspE overlap by 4 nucleotides
SO0165: gspC - general secretion pathway protein C (NCBI ptt file), at 171,350 to 172,276
gspC
SO0166: gspD - general secretion pathway protein D (NCBI ptt file), at 172,301 to 174,415
gspD
SO0167: gspE - general secretion pathway protein E (NCBI ptt file), at 174,412 to 175,977
gspE
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 171.305 kb on - strand at 171.329 kb on + strand at 171.361 kb on + strand at 171.387 kb on - strand at 171.453 kb on - strand, within gspC at 171.513 kb on - strand, within gspC at 171.518 kb on - strand, within gspC at 171.520 kb on + strand, within gspC at 171.528 kb on - strand, within gspC at 171.537 kb on - strand, within gspC at 171.538 kb on - strand, within gspC at 171.626 kb on + strand, within gspC at 171.634 kb on - strand, within gspC at 171.655 kb on - strand, within gspC at 171.663 kb on - strand, within gspC at 171.812 kb on + strand, within gspC at 171.834 kb on + strand, within gspC at 171.867 kb on - strand, within gspC at 171.931 kb on - strand, within gspC at 171.963 kb on + strand, within gspC at 172.073 kb on - strand, within gspC at 172.096 kb on - strand, within gspC at 172.096 kb on - strand, within gspC at 172.130 kb on - strand, within gspC at 172.315 kb on + strand at 172.401 kb on + strand at 172.509 kb on + strand at 172.514 kb on - strand, within gspD at 172.516 kb on + strand, within gspD at 172.562 kb on + strand, within gspD at 172.575 kb on - strand, within gspD at 172.667 kb on + strand, within gspD at 172.667 kb on + strand, within gspD at 172.667 kb on + strand, within gspD at 172.675 kb on - strand, within gspD at 172.700 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.705 kb on + strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.713 kb on - strand, within gspD at 172.718 kb on - strand, within gspD at 172.741 kb on - strand, within gspD at 172.775 kb on - strand, within gspD at 172.791 kb on + strand, within gspD at 172.799 kb on - strand, within gspD at 172.812 kb on - strand, within gspD at 172.940 kb on + strand, within gspD at 172.948 kb on - strand, within gspD at 172.948 kb on - strand, within gspD at 172.948 kb on - strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.011 kb on + strand, within gspD at 173.019 kb on - strand, within gspD at 173.019 kb on - strand, within gspD at 173.019 kb on - strand, within gspD at 173.055 kb on + strand, within gspD at 173.087 kb on + strand, within gspD at 173.087 kb on + strand, within gspD at 173.092 kb on + strand, within gspD at 173.101 kb on + strand, within gspD at 173.107 kb on + strand, within gspD at 173.129 kb on - strand, within gspD at 173.146 kb on - strand, within gspD at 173.146 kb on - strand, within gspD at 173.156 kb on + strand, within gspD at 173.156 kb on - strand, within gspD at 173.174 kb on - strand, within gspD at 173.283 kb on + strand, within gspD at 173.283 kb on + strand, within gspD at 173.283 kb on + strand, within gspD at 173.283 kb on + strand, within gspD at 173.286 kb on - strand, within gspD at 173.291 kb on - strand, within gspD at 173.291 kb on - strand, within gspD at 173.291 kb on - strand, within gspD at 173.307 kb on + strand, within gspD at 173.317 kb on - strand, within gspD at 173.364 kb on - strand, within gspD at 173.398 kb on + strand, within gspD at 173.416 kb on - strand, within gspD at 173.434 kb on - strand, within gspD at 173.500 kb on + strand, within gspD at 173.500 kb on + strand, within gspD at 173.588 kb on + strand, within gspD at 173.606 kb on + strand, within gspD at 173.679 kb on + strand, within gspD at 173.705 kb on - strand, within gspD at 173.781 kb on + strand, within gspD at 173.808 kb on + strand, within gspD at 173.836 kb on + strand, within gspD at 173.871 kb on + strand, within gspD at 174.092 kb on + strand, within gspD at 174.220 kb on + strand at 174.271 kb on + strand at 174.271 kb on + strand at 174.275 kb on - strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.352 kb on + strand at 174.360 kb on - strand at 174.588 kb on + strand, within gspE at 174.646 kb on - strand, within gspE at 174.654 kb on + strand, within gspE at 174.669 kb on - strand, within gspE at 174.702 kb on + strand, within gspE at 174.733 kb on - strand, within gspE at 174.797 kb on + strand, within gspE at 174.807 kb on + strand, within gspE at 174.815 kb on - strand, within gspE at 174.833 kb on + strand, within gspE at 174.843 kb on + strand, within gspE at 174.866 kb on - strand, within gspE at 174.953 kb on - strand, within gspE at 174.976 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 175.007 kb on - strand, within gspE at 175.013 kb on - strand, within gspE at 175.269 kb on + strand, within gspE at 175.285 kb on - strand, within gspE at 175.407 kb on - strand, within gspE
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 171,305 - -4.1 171,329 + -2.6 171,361 + -0.5 171,387 - -2.5 171,453 - gspC SO0165 0.11 -1.5 171,513 - gspC SO0165 0.18 -1.9 171,518 - gspC SO0165 0.18 +0.1 171,520 + gspC SO0165 0.18 -3.7 171,528 - gspC SO0165 0.19 -1.8 171,537 - gspC SO0165 0.20 -2.4 171,538 - gspC SO0165 0.20 -3.1 171,626 + gspC SO0165 0.30 -2.4 171,634 - gspC SO0165 0.31 -1.9 171,655 - gspC SO0165 0.33 -0.5 171,663 - gspC SO0165 0.34 -2.8 171,812 + gspC SO0165 0.50 -3.0 171,834 + gspC SO0165 0.52 -2.8 171,867 - gspC SO0165 0.56 -2.1 171,931 - gspC SO0165 0.63 -2.6 171,963 + gspC SO0165 0.66 -2.1 172,073 - gspC SO0165 0.78 -3.7 172,096 - gspC SO0165 0.80 -1.0 172,096 - gspC SO0165 0.80 -1.2 172,130 - gspC SO0165 0.84 -2.4 172,315 + -2.3 172,401 + -2.7 172,509 + -0.7 172,514 - gspD SO0166 0.10 -2.7 172,516 + gspD SO0166 0.10 -1.7 172,562 + gspD SO0166 0.12 -3.0 172,575 - gspD SO0166 0.13 -2.6 172,667 + gspD SO0166 0.17 -2.7 172,667 + gspD SO0166 0.17 -3.2 172,667 + gspD SO0166 0.17 -3.3 172,675 - gspD SO0166 0.18 -2.0 172,700 + gspD SO0166 0.19 -0.7 172,705 + gspD SO0166 0.19 -4.1 172,705 + gspD SO0166 0.19 -1.8 172,705 + gspD SO0166 0.19 -3.0 172,705 + gspD SO0166 0.19 -3.2 172,705 + gspD SO0166 0.19 -2.0 172,705 + gspD SO0166 0.19 +0.1 172,705 + gspD SO0166 0.19 -0.2 172,713 - gspD SO0166 0.19 -1.7 172,713 - gspD SO0166 0.19 -4.4 172,713 - gspD SO0166 0.19 -3.3 172,713 - gspD SO0166 0.19 -2.7 172,713 - gspD SO0166 0.19 -1.6 172,713 - gspD SO0166 0.19 -3.2 172,713 - gspD SO0166 0.19 -2.6 172,718 - gspD SO0166 0.20 -3.7 172,741 - gspD SO0166 0.21 -1.0 172,775 - gspD SO0166 0.22 -0.5 172,791 + gspD SO0166 0.23 -2.3 172,799 - gspD SO0166 0.24 -1.7 172,812 - gspD SO0166 0.24 -1.9 172,940 + gspD SO0166 0.30 -1.6 172,948 - gspD SO0166 0.31 -1.9 172,948 - gspD SO0166 0.31 -2.7 172,948 - gspD SO0166 0.31 -3.3 173,011 + gspD SO0166 0.34 +0.8 173,011 + gspD SO0166 0.34 -3.2 173,011 + gspD SO0166 0.34 -2.6 173,011 + gspD SO0166 0.34 -1.9 173,019 - gspD SO0166 0.34 -2.1 173,019 - gspD SO0166 0.34 -2.3 173,019 - gspD SO0166 0.34 -1.8 173,055 + gspD SO0166 0.36 -0.7 173,087 + gspD SO0166 0.37 -3.1 173,087 + gspD SO0166 0.37 -1.6 173,092 + gspD SO0166 0.37 -2.4 173,101 + gspD SO0166 0.38 -1.8 173,107 + gspD SO0166 0.38 -0.7 173,129 - gspD SO0166 0.39 -2.8 173,146 - gspD SO0166 0.40 -3.6 173,146 - gspD SO0166 0.40 -2.0 173,156 + gspD SO0166 0.40 -2.2 173,156 - gspD SO0166 0.40 -1.9 173,174 - gspD SO0166 0.41 -0.2 173,283 + gspD SO0166 0.46 -2.7 173,283 + gspD SO0166 0.46 -2.0 173,283 + gspD SO0166 0.46 -3.6 173,283 + gspD SO0166 0.46 -3.4 173,286 - gspD SO0166 0.47 -2.2 173,291 - gspD SO0166 0.47 -3.0 173,291 - gspD SO0166 0.47 -0.2 173,291 - gspD SO0166 0.47 -2.3 173,307 + gspD SO0166 0.48 -0.9 173,317 - gspD SO0166 0.48 -2.2 173,364 - gspD SO0166 0.50 -3.5 173,398 + gspD SO0166 0.52 +0.8 173,416 - gspD SO0166 0.53 -3.3 173,434 - gspD SO0166 0.54 -3.1 173,500 + gspD SO0166 0.57 +1.1 173,500 + gspD SO0166 0.57 -2.0 173,588 + gspD SO0166 0.61 -0.2 173,606 + gspD SO0166 0.62 +1.7 173,679 + gspD SO0166 0.65 -1.8 173,705 - gspD SO0166 0.66 -1.7 173,781 + gspD SO0166 0.70 +1.8 173,808 + gspD SO0166 0.71 -2.9 173,836 + gspD SO0166 0.73 -2.0 173,871 + gspD SO0166 0.74 -2.4 174,092 + gspD SO0166 0.85 -2.0 174,220 + -0.9 174,271 + -0.4 174,271 + -0.2 174,275 - -1.5 174,335 + -1.2 174,335 + -1.8 174,335 + -2.6 174,335 + -2.8 174,352 + +2.0 174,360 - -2.0 174,588 + gspE SO0167 0.11 -3.7 174,646 - gspE SO0167 0.15 -3.5 174,654 + gspE SO0167 0.15 +1.1 174,669 - gspE SO0167 0.16 -1.7 174,702 + gspE SO0167 0.19 -1.5 174,733 - gspE SO0167 0.20 -2.0 174,797 + gspE SO0167 0.25 -1.6 174,807 + gspE SO0167 0.25 -3.7 174,815 - gspE SO0167 0.26 -0.5 174,833 + gspE SO0167 0.27 -1.5 174,843 + gspE SO0167 0.28 -3.0 174,866 - gspE SO0167 0.29 -3.7 174,953 - gspE SO0167 0.35 -0.7 174,976 + gspE SO0167 0.36 -3.4 174,999 + gspE SO0167 0.37 -1.7 174,999 + gspE SO0167 0.37 -2.0 174,999 + gspE SO0167 0.37 -2.4 175,007 - gspE SO0167 0.38 -1.3 175,013 - gspE SO0167 0.38 -1.9 175,269 + gspE SO0167 0.55 -3.3 175,285 - gspE SO0167 0.56 -3.4 175,407 - gspE SO0167 0.64 -1.2
Or see this region's nucleotide sequence