Experiment: Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01525 and BWI76_RS01530 are separated by 318 nucleotides BWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotides BWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotides BWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides
BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235
_RS01525
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454
_RS01535
BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656
_RS01540
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
Position (kb) 312
313
314 Strain fitness (log2 ratio) -2
-1
0
1
2 at 311.588 kb on + strand, within BWI76_RS01525 at 311.657 kb on - strand, within BWI76_RS01525 at 311.679 kb on - strand, within BWI76_RS01525 at 311.712 kb on + strand, within BWI76_RS01525 at 311.720 kb on - strand, within BWI76_RS01525 at 311.722 kb on + strand, within BWI76_RS01525 at 311.742 kb on - strand, within BWI76_RS01525 at 311.763 kb on + strand, within BWI76_RS01525 at 311.781 kb on - strand, within BWI76_RS01525 at 311.840 kb on + strand, within BWI76_RS01525 at 312.239 kb on - strand at 312.259 kb on + strand at 312.265 kb on - strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.337 kb on - strand at 312.392 kb on + strand at 312.399 kb on + strand at 312.406 kb on - strand at 312.416 kb on - strand at 312.435 kb on + strand at 312.443 kb on - strand at 312.443 kb on - strand at 312.452 kb on + strand at 312.460 kb on - strand at 312.462 kb on + strand at 312.488 kb on + strand at 312.562 kb on + strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.577 kb on + strand at 312.587 kb on + strand at 312.596 kb on - strand at 312.643 kb on + strand at 312.666 kb on + strand at 312.674 kb on - strand, within BWI76_RS01530 at 312.674 kb on - strand, within BWI76_RS01530 at 312.941 kb on + strand, within BWI76_RS01530 at 313.038 kb on + strand, within BWI76_RS01530 at 313.466 kb on + strand, within BWI76_RS01530 at 313.636 kb on + strand at 313.636 kb on + strand at 313.716 kb on + strand at 313.846 kb on + strand at 313.849 kb on - strand, within BWI76_RS01535 at 313.908 kb on - strand, within BWI76_RS01535 at 314.057 kb on - strand, within BWI76_RS01535 at 314.068 kb on - strand, within BWI76_RS01535 at 314.080 kb on + strand, within BWI76_RS01535 at 314.178 kb on + strand, within BWI76_RS01535 at 314.257 kb on - strand, within BWI76_RS01535 at 314.337 kb on + strand, within BWI76_RS01535 at 314.624 kb on + strand, within BWI76_RS01540 at 314.624 kb on + strand, within BWI76_RS01540
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM remove 311,588 + BWI76_RS01525 0.58 -0.1 311,657 - BWI76_RS01525 0.62 +1.1 311,679 - BWI76_RS01525 0.64 +0.4 311,712 + BWI76_RS01525 0.66 +0.9 311,720 - BWI76_RS01525 0.66 +0.1 311,722 + BWI76_RS01525 0.66 +0.9 311,742 - BWI76_RS01525 0.68 +0.3 311,763 + BWI76_RS01525 0.69 +0.6 311,781 - BWI76_RS01525 0.70 +0.0 311,840 + BWI76_RS01525 0.74 -0.1 312,239 - +0.2 312,259 + +0.7 312,265 - -0.5 312,282 + -0.9 312,282 + -0.2 312,282 + -1.4 312,282 + +0.7 312,282 + -1.9 312,282 + -0.4 312,282 + -1.2 312,282 + -0.7 312,282 + +0.9 312,290 - -0.0 312,290 - -0.3 312,290 - -1.7 312,290 - +0.5 312,290 - +0.4 312,290 - -0.2 312,290 - -1.4 312,337 - -1.2 312,392 + -2.4 312,399 + -0.1 312,406 - -0.2 312,416 - -1.0 312,435 + +0.2 312,443 - +0.4 312,443 - +0.5 312,452 + +0.6 312,460 - -1.5 312,462 + +0.2 312,488 + +0.3 312,562 + -0.5 312,570 - +0.2 312,570 - -0.0 312,570 - -0.2 312,577 + -0.9 312,587 + -1.2 312,596 - +2.1 312,643 + -1.0 312,666 + -0.1 312,674 - BWI76_RS01530 0.11 -1.4 312,674 - BWI76_RS01530 0.11 -0.7 312,941 + BWI76_RS01530 0.34 -0.5 313,038 + BWI76_RS01530 0.43 -1.4 313,466 + BWI76_RS01530 0.80 -1.5 313,636 + -1.7 313,636 + -0.9 313,716 + -0.3 313,846 + -0.9 313,849 - BWI76_RS01535 0.21 -1.6 313,908 - BWI76_RS01535 0.29 -1.1 314,057 - BWI76_RS01535 0.48 +0.4 314,068 - BWI76_RS01535 0.50 -0.9 314,080 + BWI76_RS01535 0.51 -1.3 314,178 + BWI76_RS01535 0.64 -1.3 314,257 - BWI76_RS01535 0.74 +1.1 314,337 + BWI76_RS01535 0.85 +0.7 314,624 + BWI76_RS01540 0.84 +0.4 314,624 + BWI76_RS01540 0.84 -0.3
Or see this region's nucleotide sequence