Experiment: MoLS4 with low Ammonium 0.2mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0926 and rplU are separated by 116 nucleotides rplU and rpmA are separated by 20 nucleotides rpmA and obg are separated by 148 nucleotides obg and proB are separated by 17 nucleotides
DVU0926: DVU0926 - hypothetical protein (TIGR), at 1,017,547 to 1,017,738
DVU0926
DVU0927: rplU - ribosomal protein L21 (TIGR), at 1,017,855 to 1,018,163
rplU
DVU0928: rpmA - ribosomal protein L27 (TIGR), at 1,018,184 to 1,018,447
rpmA
DVU0929: obg - GTP-binding protein, GTP1/OBG family (TIGR), at 1,018,596 to 1,019,696
obg
DVU0930: proB - glutamate 5-kinase (TIGR), at 1,019,714 to 1,020,856
proB
Position (kb)
1018
1019
1020 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1017.658 kb on - strand, within DVU0926 at 1017.688 kb on + strand, within DVU0926 at 1017.704 kb on + strand, within DVU0926 at 1017.752 kb on - strand at 1017.752 kb on - strand at 1017.752 kb on - strand at 1019.627 kb on - strand at 1019.749 kb on - strand at 1019.975 kb on + strand, within proB at 1019.975 kb on + strand, within proB at 1019.983 kb on - strand, within proB at 1020.305 kb on - strand, within proB at 1020.333 kb on + strand, within proB at 1020.353 kb on + strand, within proB at 1020.411 kb on + strand, within proB at 1020.424 kb on - strand, within proB at 1020.598 kb on - strand, within proB at 1020.668 kb on - strand, within proB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with low Ammonium 0.2mM (N) remove 1,017,658 - DVU0926 0.58 -2.5 1,017,688 + DVU0926 0.73 -0.7 1,017,704 + DVU0926 0.82 -0.2 1,017,752 - -0.2 1,017,752 - -1.3 1,017,752 - +3.5 1,019,627 - -0.9 1,019,749 - -3.0 1,019,975 + proB DVU0930 0.23 -3.0 1,019,975 + proB DVU0930 0.23 -0.4 1,019,983 - proB DVU0930 0.24 -2.9 1,020,305 - proB DVU0930 0.52 -0.0 1,020,333 + proB DVU0930 0.54 -1.4 1,020,353 + proB DVU0930 0.56 -1.0 1,020,411 + proB DVU0930 0.61 -3.4 1,020,424 - proB DVU0930 0.62 -0.4 1,020,598 - proB DVU0930 0.77 -0.4 1,020,668 - proB DVU0930 0.83 -3.8
Or see this region's nucleotide sequence