Strain Fitness in Shewanella oneidensis MR-1 around SO4670

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpolA and elbB are separated by 385 nucleotideselbB and glpG are separated by 79 nucleotidesglpG and glpE overlap by 4 nucleotides SO4669: polA - DNA polymerase I (NCBI ptt file), at 4,865,820 to 4,868,588 polA SO4670: elbB - enhancing lycopene biosynthesis protein (NCBI ptt file), at 4,868,974 to 4,869,630 elbB SO4671: glpG - glpG protein (NCBI ptt file), at 4,869,710 to 4,870,552 glpG SO4672: glpE - glpE protein (NCBI ptt file), at 4,870,549 to 4,870,854 glpE Position (kb) 4868 4869 4870Strain fitness (log2 ratio) -2 -1 0 1at 4868.645 kb on + strandat 4868.645 kb on + strandat 4868.722 kb on + strandat 4868.730 kb on - strandat 4868.730 kb on - strandat 4869.195 kb on - strand, within elbBat 4869.305 kb on + strand, within elbBat 4869.399 kb on - strand, within elbBat 4869.447 kb on + strand, within elbBat 4869.489 kb on + strand, within elbBat 4869.501 kb on + strand, within elbBat 4869.507 kb on - strand, within elbBat 4869.574 kb on - strandat 4869.584 kb on - strandat 4869.610 kb on - strandat 4869.652 kb on - strandat 4869.658 kb on - strandat 4869.667 kb on + strandat 4869.669 kb on - strandat 4869.748 kb on + strandat 4869.838 kb on + strand, within glpGat 4869.877 kb on + strand, within glpGat 4869.882 kb on + strand, within glpGat 4869.890 kb on - strand, within glpGat 4869.890 kb on - strand, within glpGat 4869.913 kb on - strand, within glpGat 4869.938 kb on - strand, within glpGat 4869.949 kb on + strand, within glpGat 4869.968 kb on + strand, within glpGat 4869.986 kb on - strand, within glpGat 4869.986 kb on - strand, within glpGat 4869.998 kb on - strand, within glpGat 4870.119 kb on - strand, within glpGat 4870.191 kb on + strand, within glpGat 4870.202 kb on + strand, within glpGat 4870.218 kb on + strand, within glpGat 4870.342 kb on + strand, within glpGat 4870.342 kb on + strand, within glpGat 4870.350 kb on - strand, within glpGat 4870.488 kb on + strandat 4870.488 kb on + strandat 4870.496 kb on - strandat 4870.503 kb on + strandat 4870.508 kb on + strandat 4870.516 kb on - strandat 4870.544 kb on - strandat 4870.610 kb on - strand, within glpE

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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4,868,645 + +0.9
4,868,645 + -0.7
4,868,722 + +0.2
4,868,730 - -0.0
4,868,730 - +0.4
4,869,195 - elbB SO4670 0.34 +0.3
4,869,305 + elbB SO4670 0.50 -2.5
4,869,399 - elbB SO4670 0.65 -0.8
4,869,447 + elbB SO4670 0.72 -0.6
4,869,489 + elbB SO4670 0.78 -1.3
4,869,501 + elbB SO4670 0.80 -0.3
4,869,507 - elbB SO4670 0.81 +0.4
4,869,574 - +0.6
4,869,584 - -0.6
4,869,610 - -0.2
4,869,652 - +1.0
4,869,658 - -1.6
4,869,667 + -0.6
4,869,669 - +0.0
4,869,748 + +0.7
4,869,838 + glpG SO4671 0.15 -0.3
4,869,877 + glpG SO4671 0.20 -1.5
4,869,882 + glpG SO4671 0.20 -0.1
4,869,890 - glpG SO4671 0.21 +0.0
4,869,890 - glpG SO4671 0.21 +0.6
4,869,913 - glpG SO4671 0.24 +0.3
4,869,938 - glpG SO4671 0.27 +0.5
4,869,949 + glpG SO4671 0.28 -0.7
4,869,968 + glpG SO4671 0.31 +0.4
4,869,986 - glpG SO4671 0.33 -1.1
4,869,986 - glpG SO4671 0.33 +0.1
4,869,998 - glpG SO4671 0.34 -0.0
4,870,119 - glpG SO4671 0.49 -0.3
4,870,191 + glpG SO4671 0.57 -0.4
4,870,202 + glpG SO4671 0.58 -0.3
4,870,218 + glpG SO4671 0.60 +0.3
4,870,342 + glpG SO4671 0.75 -1.2
4,870,342 + glpG SO4671 0.75 -0.1
4,870,350 - glpG SO4671 0.76 -0.2
4,870,488 + -0.2
4,870,488 + +1.0
4,870,496 - -0.6
4,870,503 + +0.9
4,870,508 + -0.5
4,870,516 - -0.1
4,870,544 - +0.4
4,870,610 - glpE SO4672 0.20 +0.4

Or see this region's nucleotide sequence