Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mshE and SO4110 overlap by 4 nucleotides SO4110 and mshM overlap by 4 nucleotides mshM and mshL are separated by 6 nucleotides
SO4109: mshE - MSHA biogenesis protein MshE (NCBI ptt file), at 4,266,832 to 4,268,592
mshE
SO4110: SO4110 - MSHA biogenesis protein MshN, putative (NCBI ptt file), at 4,268,589 to 4,269,878
SO4110
SO4111: mshM - MSHA biogenesis protein MshM (NCBI ptt file), at 4,269,875 to 4,270,807
mshM
SO4112: mshL - MSHA biogenesis protein MshL (NCBI ptt file), at 4,270,814 to 4,272,496
mshL
Position (kb)
4268
4269
4270 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4267.649 kb on + strand, within mshE at 4267.657 kb on - strand, within mshE at 4267.657 kb on - strand, within mshE at 4267.657 kb on - strand, within mshE at 4267.657 kb on - strand, within mshE at 4267.901 kb on + strand, within mshE at 4267.921 kb on + strand, within mshE at 4267.976 kb on + strand, within mshE at 4268.193 kb on + strand, within mshE at 4268.294 kb on - strand, within mshE at 4268.364 kb on + strand, within mshE at 4268.364 kb on + strand, within mshE at 4268.364 kb on + strand, within mshE at 4268.364 kb on + strand, within mshE at 4268.364 kb on + strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.372 kb on - strand, within mshE at 4268.374 kb on + strand, within mshE at 4268.396 kb on + strand, within mshE at 4268.492 kb on + strand at 4268.500 kb on - strand at 4268.500 kb on - strand at 4268.500 kb on - strand at 4268.500 kb on - strand at 4268.500 kb on - strand at 4268.500 kb on - strand at 4268.507 kb on + strand at 4268.507 kb on + strand at 4268.515 kb on - strand at 4268.651 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.661 kb on + strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.669 kb on - strand at 4268.722 kb on - strand, within SO4110 at 4268.724 kb on + strand, within SO4110 at 4268.724 kb on + strand, within SO4110 at 4268.732 kb on - strand, within SO4110 at 4268.750 kb on + strand, within SO4110 at 4268.892 kb on + strand, within SO4110 at 4268.905 kb on - strand, within SO4110 at 4268.954 kb on + strand, within SO4110 at 4268.954 kb on - strand, within SO4110 at 4269.085 kb on - strand, within SO4110 at 4269.090 kb on + strand, within SO4110 at 4269.192 kb on + strand, within SO4110 at 4269.220 kb on + strand, within SO4110 at 4269.249 kb on + strand, within SO4110 at 4269.257 kb on - strand, within SO4110 at 4269.369 kb on - strand, within SO4110 at 4269.496 kb on - strand, within SO4110 at 4269.496 kb on - strand, within SO4110 at 4269.583 kb on + strand, within SO4110 at 4269.598 kb on - strand, within SO4110 at 4269.739 kb on - strand, within SO4110 at 4269.759 kb on + strand at 4269.831 kb on + strand at 4269.886 kb on + strand at 4269.888 kb on + strand at 4269.940 kb on - strand at 4269.980 kb on + strand, within mshM at 4269.988 kb on - strand, within mshM at 4270.000 kb on - strand, within mshM at 4270.071 kb on - strand, within mshM at 4270.150 kb on + strand, within mshM at 4270.160 kb on + strand, within mshM at 4270.189 kb on + strand, within mshM at 4270.255 kb on + strand, within mshM at 4270.277 kb on + strand, within mshM at 4270.414 kb on - strand, within mshM at 4270.414 kb on - strand, within mshM at 4270.424 kb on + strand, within mshM at 4270.424 kb on - strand, within mshM at 4270.432 kb on - strand, within mshM at 4270.441 kb on + strand, within mshM at 4270.480 kb on + strand, within mshM at 4270.542 kb on + strand, within mshM at 4270.550 kb on - strand, within mshM at 4270.555 kb on - strand, within mshM at 4270.555 kb on - strand, within mshM at 4270.580 kb on - strand, within mshM at 4270.590 kb on - strand, within mshM at 4270.726 kb on - strand at 4270.729 kb on + strand at 4270.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 4,267,649 + mshE SO4109 0.46 -2.3 4,267,657 - mshE SO4109 0.47 -0.7 4,267,657 - mshE SO4109 0.47 -1.7 4,267,657 - mshE SO4109 0.47 -0.1 4,267,657 - mshE SO4109 0.47 -1.3 4,267,901 + mshE SO4109 0.61 -1.6 4,267,921 + mshE SO4109 0.62 -1.3 4,267,976 + mshE SO4109 0.65 -1.9 4,268,193 + mshE SO4109 0.77 -2.1 4,268,294 - mshE SO4109 0.83 -2.8 4,268,364 + mshE SO4109 0.87 -1.9 4,268,364 + mshE SO4109 0.87 -1.1 4,268,364 + mshE SO4109 0.87 -2.0 4,268,364 + mshE SO4109 0.87 -0.1 4,268,364 + mshE SO4109 0.87 -0.8 4,268,372 - mshE SO4109 0.87 -2.2 4,268,372 - mshE SO4109 0.87 -1.4 4,268,372 - mshE SO4109 0.87 -1.6 4,268,372 - mshE SO4109 0.87 -0.6 4,268,372 - mshE SO4109 0.87 -0.9 4,268,372 - mshE SO4109 0.87 -1.1 4,268,374 + mshE SO4109 0.88 -3.6 4,268,396 + mshE SO4109 0.89 -2.0 4,268,492 + -1.1 4,268,500 - +0.3 4,268,500 - -1.0 4,268,500 - -2.0 4,268,500 - -0.7 4,268,500 - -1.2 4,268,500 - -1.7 4,268,507 + -2.1 4,268,507 + -2.1 4,268,515 - +0.0 4,268,651 + -2.7 4,268,661 + +0.9 4,268,661 + -1.3 4,268,661 + -1.3 4,268,661 + -1.3 4,268,661 + -0.7 4,268,661 + -1.4 4,268,661 + -2.4 4,268,669 - +0.1 4,268,669 - -1.9 4,268,669 - -2.0 4,268,669 - -0.1 4,268,669 - -0.1 4,268,669 - -1.5 4,268,669 - +0.4 4,268,722 - SO4110 0.10 -0.9 4,268,724 + SO4110 0.10 -3.2 4,268,724 + SO4110 0.10 -1.6 4,268,732 - SO4110 0.11 +0.5 4,268,750 + SO4110 0.12 +0.5 4,268,892 + SO4110 0.23 -0.8 4,268,905 - SO4110 0.24 +1.9 4,268,954 + SO4110 0.28 -2.1 4,268,954 - SO4110 0.28 +0.3 4,269,085 - SO4110 0.38 +0.3 4,269,090 + SO4110 0.39 +0.5 4,269,192 + SO4110 0.47 -1.2 4,269,220 + SO4110 0.49 -1.7 4,269,249 + SO4110 0.51 -0.7 4,269,257 - SO4110 0.52 -0.1 4,269,369 - SO4110 0.60 -0.1 4,269,496 - SO4110 0.70 -1.3 4,269,496 - SO4110 0.70 -1.1 4,269,583 + SO4110 0.77 -1.9 4,269,598 - SO4110 0.78 -2.1 4,269,739 - SO4110 0.89 -1.1 4,269,759 + -1.5 4,269,831 + -1.9 4,269,886 + -0.9 4,269,888 + -4.2 4,269,940 - -1.8 4,269,980 + mshM SO4111 0.11 -1.9 4,269,988 - mshM SO4111 0.12 -1.4 4,270,000 - mshM SO4111 0.13 +0.1 4,270,071 - mshM SO4111 0.21 -0.2 4,270,150 + mshM SO4111 0.29 +0.2 4,270,160 + mshM SO4111 0.31 -2.0 4,270,189 + mshM SO4111 0.34 -1.5 4,270,255 + mshM SO4111 0.41 -0.8 4,270,277 + mshM SO4111 0.43 -2.2 4,270,414 - mshM SO4111 0.58 -1.1 4,270,414 - mshM SO4111 0.58 -1.8 4,270,424 + mshM SO4111 0.59 -0.9 4,270,424 - mshM SO4111 0.59 +0.2 4,270,432 - mshM SO4111 0.60 -0.6 4,270,441 + mshM SO4111 0.61 -0.8 4,270,480 + mshM SO4111 0.65 -0.9 4,270,542 + mshM SO4111 0.71 -2.3 4,270,550 - mshM SO4111 0.72 -1.5 4,270,555 - mshM SO4111 0.73 +0.1 4,270,555 - mshM SO4111 0.73 -0.2 4,270,580 - mshM SO4111 0.76 -1.1 4,270,590 - mshM SO4111 0.77 -2.1 4,270,726 - -1.7 4,270,729 + -1.8 4,270,848 + -1.7
Or see this region's nucleotide sequence