Strain Fitness in Shewanella oneidensis MR-1 around SO1644

Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxB and rnhB overlap by 1 nucleotidesrnhB and dnaE are separated by 116 nucleotidesdnaE and mesJ are separated by 52 nucleotides SO1642: lpxB - lipid A disaccharide synthase (NCBI ptt file), at 1,724,404 to 1,725,561 lpxB SO1643: rnhB - ribonuclease HII (NCBI ptt file), at 1,725,561 to 1,726,190 rnhB SO1644: dnaE - DNA polymerase III, alpha subunit (NCBI ptt file), at 1,726,307 to 1,729,783 dnaE SO1645: mesJ - cell cycle protein MesJ (NCBI ptt file), at 1,729,836 to 1,731,230 mesJ Position (kb) 1726 1727 1728 1729 1730Strain fitness (log2 ratio) -2 -1 0 1 2at 1725.594 kb on + strandat 1725.673 kb on - strand, within rnhBat 1725.800 kb on + strand, within rnhBat 1725.800 kb on + strand, within rnhBat 1725.803 kb on - strand, within rnhBat 1725.808 kb on - strand, within rnhBat 1725.867 kb on + strand, within rnhBat 1726.082 kb on + strand, within rnhBat 1726.082 kb on + strand, within rnhBat 1726.090 kb on - strand, within rnhBat 1726.090 kb on - strand, within rnhBat 1726.090 kb on - strand, within rnhBat 1729.787 kb on + strandat 1729.793 kb on + strandat 1729.803 kb on + strandat 1729.810 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator
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1,725,594 + +0.6
1,725,673 - rnhB SO1643 0.18 -2.1
1,725,800 + rnhB SO1643 0.38 -0.5
1,725,800 + rnhB SO1643 0.38 +0.2
1,725,803 - rnhB SO1643 0.38 -0.5
1,725,808 - rnhB SO1643 0.39 -0.4
1,725,867 + rnhB SO1643 0.49 +0.1
1,726,082 + rnhB SO1643 0.83 -0.6
1,726,082 + rnhB SO1643 0.83 -0.8
1,726,090 - rnhB SO1643 0.84 -0.7
1,726,090 - rnhB SO1643 0.84 -1.8
1,726,090 - rnhB SO1643 0.84 -1.6
1,729,787 + +2.2
1,729,793 + -0.4
1,729,803 + +0.4
1,729,810 + +0.3

Or see this region's nucleotide sequence