Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspI and gspJ overlap by 20 nucleotides gspJ and gspK overlap by 4 nucleotides gspK and gspL are separated by 48 nucleotides
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
Position (kb) 179
180
181 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ at 179.303 kb on - strand, within gspJ at 179.394 kb on + strand at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK at 179.645 kb on + strand, within gspK at 179.785 kb on + strand, within gspK at 179.849 kb on - strand, within gspK at 179.892 kb on + strand, within gspK at 180.055 kb on + strand, within gspK at 180.102 kb on - strand, within gspK at 180.209 kb on - strand, within gspK at 180.410 kb on + strand at 180.418 kb on - strand at 180.424 kb on + strand at 180.476 kb on - strand at 180.493 kb on + strand at 180.538 kb on + strand at 180.546 kb on - strand at 180.546 kb on - strand at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 178,440 + gspI SO0171 0.45 -2.1 178,516 - gspI SO0171 0.66 -0.7 178,605 - gspI SO0171 0.90 -0.2 178,654 + -1.2 178,654 + -1.9 178,654 + -4.0 178,654 + -2.6 178,654 + -1.7 178,654 + -3.3 178,662 - -2.9 178,662 - -1.2 178,700 + gspJ SO0172 0.10 -1.0 178,730 - gspJ SO0172 0.14 -1.6 178,742 - gspJ SO0172 0.15 -3.3 178,744 + gspJ SO0172 0.16 +1.0 178,816 + gspJ SO0172 0.25 -2.3 178,824 - gspJ SO0172 0.26 -1.2 178,849 - gspJ SO0172 0.29 -1.4 178,850 + gspJ SO0172 0.30 -1.5 178,867 + gspJ SO0172 0.32 -0.0 178,943 + gspJ SO0172 0.42 -2.7 178,943 + gspJ SO0172 0.42 -2.3 178,950 - gspJ SO0172 0.43 -1.2 178,951 - gspJ SO0172 0.43 -1.4 179,018 - gspJ SO0172 0.51 -2.9 179,151 + gspJ SO0172 0.69 -0.5 179,157 + gspJ SO0172 0.70 -0.3 179,220 - gspJ SO0172 0.78 -1.9 179,273 + gspJ SO0172 0.85 +0.6 179,303 - gspJ SO0172 0.89 -0.3 179,394 + -1.2 179,436 - -3.5 179,544 - gspK SO0173 0.16 -3.2 179,635 + gspK SO0173 0.25 -4.1 179,645 + gspK SO0173 0.26 -1.8 179,785 + gspK SO0173 0.40 -2.5 179,849 - gspK SO0173 0.46 -2.3 179,892 + gspK SO0173 0.51 -2.8 180,055 + gspK SO0173 0.67 -2.5 180,102 - gspK SO0173 0.72 -1.7 180,209 - gspK SO0173 0.82 -1.0 180,410 + -0.3 180,418 - -1.7 180,424 + +1.6 180,476 - -3.4 180,493 + -2.2 180,538 + -2.9 180,546 - -1.2 180,546 - -1.6 180,569 - gspL SO0174 0.11 -1.3 180,569 - gspL SO0174 0.11 -2.2 180,569 - gspL SO0174 0.11 -1.7 180,628 - gspL SO0174 0.16 -2.5 180,628 - gspL SO0174 0.16 -1.3 180,697 - gspL SO0174 0.22 -1.8 180,910 - gspL SO0174 0.40 -3.4 180,920 + gspL SO0174 0.41 -1.7 180,932 + gspL SO0174 0.42 -2.0 181,035 + gspL SO0174 0.51 -3.3 181,078 - gspL SO0174 0.54 -1.8 181,147 - gspL SO0174 0.60 -2.0 181,225 - gspL SO0174 0.67 -1.1 181,281 - gspL SO0174 0.71 -1.6 181,311 - gspL SO0174 0.74 -0.0 181,364 + gspL SO0174 0.78 -2.6 181,364 + gspL SO0174 0.78 -1.8 181,372 - gspL SO0174 0.79 -2.8 181,372 - gspL SO0174 0.79 -0.8
Or see this region's nucleotide sequence