Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrC-1 and nqrD-1 overlap by 4 nucleotides nqrD-1 and nqrE-1 are separated by 9 nucleotides nqrE-1 and nqrF-1 are separated by 51 nucleotides
SO0904: nqrC-1 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 931,522 to 932,316
nqrC-1
SO0905: nqrD-1 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 932,313 to 932,933
nqrD-1
SO0906: nqrE-1 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 932,943 to 933,551
nqrE-1
SO0907: nqrF-1 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 933,603 to 934,820
nqrF-1
Position (kb) 932
933
934 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 932.045 kb on - strand, within nqrC-1 at 932.075 kb on + strand, within nqrC-1 at 932.136 kb on + strand, within nqrC-1 at 932.298 kb on + strand at 932.306 kb on - strand at 932.385 kb on + strand, within nqrD-1 at 932.414 kb on + strand, within nqrD-1 at 932.427 kb on - strand, within nqrD-1 at 932.446 kb on + strand, within nqrD-1 at 932.510 kb on + strand, within nqrD-1 at 932.510 kb on + strand, within nqrD-1 at 932.652 kb on + strand, within nqrD-1 at 932.660 kb on - strand, within nqrD-1 at 932.664 kb on + strand, within nqrD-1 at 932.684 kb on + strand, within nqrD-1 at 932.700 kb on + strand, within nqrD-1 at 932.708 kb on - strand, within nqrD-1 at 932.737 kb on + strand, within nqrD-1 at 932.737 kb on + strand, within nqrD-1 at 932.745 kb on - strand, within nqrD-1 at 932.745 kb on - strand, within nqrD-1 at 932.790 kb on - strand, within nqrD-1 at 932.805 kb on - strand, within nqrD-1 at 932.805 kb on - strand, within nqrD-1 at 932.927 kb on + strand at 932.961 kb on - strand at 932.961 kb on - strand at 932.970 kb on - strand at 932.979 kb on + strand at 932.980 kb on - strand at 932.997 kb on - strand at 932.999 kb on + strand at 933.015 kb on + strand, within nqrE-1 at 933.023 kb on - strand, within nqrE-1 at 933.025 kb on + strand, within nqrE-1 at 933.035 kb on + strand, within nqrE-1 at 933.138 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.259 kb on + strand, within nqrE-1 at 933.311 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.425 kb on + strand, within nqrE-1 at 933.430 kb on - strand, within nqrE-1 at 933.468 kb on + strand, within nqrE-1 at 933.469 kb on + strand, within nqrE-1 at 933.469 kb on + strand, within nqrE-1 at 933.477 kb on - strand, within nqrE-1 at 933.486 kb on - strand, within nqrE-1 at 933.513 kb on + strand at 933.557 kb on + strand at 933.634 kb on + strand at 933.647 kb on - strand at 933.701 kb on + strand at 933.828 kb on + strand, within nqrF-1 at 934.195 kb on + strand, within nqrF-1 at 934.339 kb on + strand, within nqrF-1 at 934.364 kb on + strand, within nqrF-1 at 934.486 kb on - strand, within nqrF-1
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 932,045 - nqrC-1 SO0904 0.66 -1.7 932,075 + nqrC-1 SO0904 0.70 -2.7 932,136 + nqrC-1 SO0904 0.77 -0.7 932,298 + -0.5 932,306 - +1.6 932,385 + nqrD-1 SO0905 0.12 -1.7 932,414 + nqrD-1 SO0905 0.16 -0.6 932,427 - nqrD-1 SO0905 0.18 -0.6 932,446 + nqrD-1 SO0905 0.21 -1.8 932,510 + nqrD-1 SO0905 0.32 -0.9 932,510 + nqrD-1 SO0905 0.32 -0.3 932,652 + nqrD-1 SO0905 0.55 -0.5 932,660 - nqrD-1 SO0905 0.56 -0.3 932,664 + nqrD-1 SO0905 0.57 -0.1 932,684 + nqrD-1 SO0905 0.60 -0.6 932,700 + nqrD-1 SO0905 0.62 +0.0 932,708 - nqrD-1 SO0905 0.64 +0.2 932,737 + nqrD-1 SO0905 0.68 -0.7 932,737 + nqrD-1 SO0905 0.68 -1.5 932,745 - nqrD-1 SO0905 0.70 -1.5 932,745 - nqrD-1 SO0905 0.70 -0.8 932,790 - nqrD-1 SO0905 0.77 -0.1 932,805 - nqrD-1 SO0905 0.79 -0.2 932,805 - nqrD-1 SO0905 0.79 -1.7 932,927 + -0.6 932,961 - +0.4 932,961 - +0.5 932,970 - -1.4 932,979 + -2.4 932,980 - -1.0 932,997 - -0.6 932,999 + -0.6 933,015 + nqrE-1 SO0906 0.12 -0.9 933,023 - nqrE-1 SO0906 0.13 -0.0 933,025 + nqrE-1 SO0906 0.13 -1.7 933,035 + nqrE-1 SO0906 0.15 +0.0 933,138 - nqrE-1 SO0906 0.32 -0.4 933,157 - nqrE-1 SO0906 0.35 -0.3 933,157 - nqrE-1 SO0906 0.35 -0.2 933,157 - nqrE-1 SO0906 0.35 -1.0 933,259 + nqrE-1 SO0906 0.52 -1.6 933,311 - nqrE-1 SO0906 0.60 -0.2 933,407 - nqrE-1 SO0906 0.76 -0.3 933,407 - nqrE-1 SO0906 0.76 -2.7 933,407 - nqrE-1 SO0906 0.76 -3.0 933,425 + nqrE-1 SO0906 0.79 +0.4 933,430 - nqrE-1 SO0906 0.80 -2.0 933,468 + nqrE-1 SO0906 0.86 -1.7 933,469 + nqrE-1 SO0906 0.86 -3.2 933,469 + nqrE-1 SO0906 0.86 -2.2 933,477 - nqrE-1 SO0906 0.88 -2.2 933,486 - nqrE-1 SO0906 0.89 -0.8 933,513 + +0.9 933,557 + +1.2 933,634 + -2.2 933,647 - -1.5 933,701 + -0.9 933,828 + nqrF-1 SO0907 0.18 -0.9 934,195 + nqrF-1 SO0907 0.49 -0.1 934,339 + nqrF-1 SO0907 0.60 -1.4 934,364 + nqrF-1 SO0907 0.62 -3.3 934,486 - nqrF-1 SO0907 0.72 +0.3
Or see this region's nucleotide sequence