Experiment: Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27410 and BWI76_RS27415 overlap by 6 nucleotides BWI76_RS27415 and BWI76_RS27420 are separated by 83 nucleotides BWI76_RS27420 and BWI76_RS27425 are separated by 54 nucleotides BWI76_RS27425 and BWI76_RS27430 are separated by 18 nucleotides
BWI76_RS27410: BWI76_RS27410 - DNA ligase B, at 5,687,041 to 5,688,717
_RS27410
BWI76_RS27415: BWI76_RS27415 - hypothetical protein, at 5,688,712 to 5,688,891
_RS27415
BWI76_RS27420: BWI76_RS27420 - guanylate kinase, at 5,688,975 to 5,689,598
_RS27420
BWI76_RS27425: BWI76_RS27425 - DNA-directed RNA polymerase subunit omega, at 5,689,653 to 5,689,928
_RS27425
BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067
_RS27430
Position (kb) 5689
5690 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5688.693 kb on - strand at 5688.900 kb on + strand at 5688.900 kb on + strand at 5688.908 kb on - strand at 5688.926 kb on - strand at 5688.926 kb on - strand at 5688.926 kb on - strand at 5689.709 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL remove 5,688,693 - +1.1 5,688,900 + +1.0 5,688,900 + +0.5 5,688,908 - -0.2 5,688,926 - +1.6 5,688,926 - +1.2 5,688,926 - -2.3 5,689,709 + BWI76_RS27425 0.20 -0.9 5,689,821 + BWI76_RS27425 0.61 -2.9 5,689,821 + BWI76_RS27425 0.61 +1.1
Or see this region's nucleotide sequence