Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01280

Experiment: Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01275 and BWI76_RS01280 are separated by 232 nucleotidesBWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotidesBWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748 _RS01275 BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242 _RS01280 BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097 _RS01285 BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913 _RS01290 Position (kb) 261 262 263 264 265Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 260.996 kb on + strand, within BWI76_RS01275at 261.005 kb on - strand, within BWI76_RS01275at 261.109 kb on - strand, within BWI76_RS01275at 261.114 kb on - strand, within BWI76_RS01275at 261.435 kb on - strand, within BWI76_RS01275at 261.625 kb on + strand, within BWI76_RS01275at 261.633 kb on - strand, within BWI76_RS01275at 261.663 kb on + strandat 261.699 kb on + strandat 261.904 kb on - strandat 262.023 kb on - strandat 262.089 kb on - strandat 262.183 kb on + strandat 262.201 kb on + strandat 262.226 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.504 kb on - strand, within BWI76_RS01280at 262.517 kb on - strand, within BWI76_RS01280at 262.692 kb on - strand, within BWI76_RS01280at 262.692 kb on - strand, within BWI76_RS01280at 262.901 kb on + strand, within BWI76_RS01280at 262.909 kb on - strand, within BWI76_RS01280at 263.014 kb on + strand, within BWI76_RS01280at 263.072 kb on - strand, within BWI76_RS01280at 263.247 kb on - strand, within BWI76_RS01280at 263.290 kb on + strand, within BWI76_RS01280at 263.298 kb on - strand, within BWI76_RS01280at 263.345 kb on + strand, within BWI76_RS01280at 263.406 kb on + strand, within BWI76_RS01280at 263.459 kb on - strand, within BWI76_RS01280at 263.513 kb on - strand, within BWI76_RS01280at 263.518 kb on - strand, within BWI76_RS01280at 263.525 kb on - strand, within BWI76_RS01280at 263.535 kb on - strand, within BWI76_RS01280at 263.901 kb on + strand, within BWI76_RS01280at 263.906 kb on + strand, within BWI76_RS01280at 264.050 kb on + strandat 264.087 kb on - strandat 264.104 kb on + strandat 264.142 kb on + strandat 264.217 kb on - strandat 264.217 kb on - strandat 264.217 kb on - strandat 264.319 kb on - strandat 264.367 kb on - strandat 264.448 kb on + strandat 264.505 kb on + strand, within BWI76_RS01285at 264.568 kb on - strand, within BWI76_RS01285at 264.662 kb on - strand, within BWI76_RS01285at 264.761 kb on - strand, within BWI76_RS01285at 264.958 kb on + strand, within BWI76_RS01285at 264.992 kb on + strand, within BWI76_RS01285at 265.007 kb on - strand, within BWI76_RS01285at 265.060 kb on - strandat 265.099 kb on - strandat 265.132 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL
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260,996 + BWI76_RS01275 0.21 -0.9
261,005 - BWI76_RS01275 0.22 -5.3
261,109 - BWI76_RS01275 0.33 -2.4
261,114 - BWI76_RS01275 0.33 -1.7
261,435 - BWI76_RS01275 0.67 -3.3
261,625 + BWI76_RS01275 0.87 -3.9
261,633 - BWI76_RS01275 0.88 -2.1
261,663 + -4.5
261,699 + -2.9
261,904 - -2.3
262,023 - -2.1
262,089 - -1.5
262,183 + -2.3
262,201 + -1.5
262,226 - BWI76_RS01280 0.11 -3.9
262,504 - BWI76_RS01280 0.23 -0.9
262,504 - BWI76_RS01280 0.23 -1.9
262,504 - BWI76_RS01280 0.23 -1.0
262,504 - BWI76_RS01280 0.23 -1.4
262,504 - BWI76_RS01280 0.23 -2.5
262,504 - BWI76_RS01280 0.23 -2.3
262,517 - BWI76_RS01280 0.24 -2.6
262,692 - BWI76_RS01280 0.31 -3.1
262,692 - BWI76_RS01280 0.31 -2.7
262,901 + BWI76_RS01280 0.41 -1.8
262,909 - BWI76_RS01280 0.41 -3.0
263,014 + BWI76_RS01280 0.46 -1.3
263,072 - BWI76_RS01280 0.48 -1.5
263,247 - BWI76_RS01280 0.56 -2.0
263,290 + BWI76_RS01280 0.58 -2.2
263,298 - BWI76_RS01280 0.58 -0.9
263,345 + BWI76_RS01280 0.60 -1.4
263,406 + BWI76_RS01280 0.63 -1.5
263,459 - BWI76_RS01280 0.65 +0.7
263,513 - BWI76_RS01280 0.68 -3.9
263,518 - BWI76_RS01280 0.68 -0.9
263,525 - BWI76_RS01280 0.68 -1.7
263,535 - BWI76_RS01280 0.69 -0.8
263,901 + BWI76_RS01280 0.85 -1.5
263,906 + BWI76_RS01280 0.85 -0.9
264,050 + -1.0
264,087 - -4.3
264,104 + -0.8
264,142 + -0.9
264,217 - +0.1
264,217 - -0.9
264,217 - -1.5
264,319 - -0.4
264,367 - -0.1
264,448 + +0.8
264,505 + BWI76_RS01285 0.14 +1.6
264,568 - BWI76_RS01285 0.23 +1.5
264,662 - BWI76_RS01285 0.37 -0.6
264,761 - BWI76_RS01285 0.51 +0.5
264,958 + BWI76_RS01285 0.80 -0.3
264,992 + BWI76_RS01285 0.85 +1.1
265,007 - BWI76_RS01285 0.87 +0.1
265,060 - -0.9
265,099 - -1.4
265,132 - -0.3

Or see this region's nucleotide sequence