Experiment: Glucose (C) and Betaine (N); with MOPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS00385 and PFLU_RS00390 are separated by 56 nucleotides PFLU_RS00390 and PFLU_RS00395 are separated by 74 nucleotides
PFLU_RS00385: PFLU_RS00385 - homoserine kinase, at 76,789 to 77,742
_RS00385
PFLU_RS00390: PFLU_RS00390 - DUF2782 domain-containing protein, at 77,799 to 78,089
_RS00390
PFLU_RS00395: PFLU_RS00395 - DNA polymerase I, at 78,164 to 80,941
_RS00395
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 76.804 kb on + strand at 76.804 kb on + strand at 76.804 kb on + strand at 76.804 kb on + strand at 76.804 kb on + strand at 76.804 kb on + strand at 76.805 kb on - strand at 76.805 kb on - strand at 76.805 kb on - strand at 76.805 kb on - strand at 77.081 kb on + strand, within PFLU_RS00385 at 77.108 kb on + strand, within PFLU_RS00385 at 77.325 kb on + strand, within PFLU_RS00385 at 77.325 kb on + strand, within PFLU_RS00385 at 77.326 kb on - strand, within PFLU_RS00385 at 77.746 kb on + strand at 77.759 kb on - strand at 77.792 kb on + strand at 77.793 kb on - strand at 77.793 kb on - strand at 77.793 kb on - strand at 77.838 kb on - strand, within PFLU_RS00390 at 77.887 kb on - strand, within PFLU_RS00390 at 77.896 kb on + strand, within PFLU_RS00390 at 77.896 kb on + strand, within PFLU_RS00390 at 77.896 kb on + strand, within PFLU_RS00390 at 77.896 kb on + strand, within PFLU_RS00390 at 77.896 kb on + strand, within PFLU_RS00390 at 77.897 kb on - strand, within PFLU_RS00390 at 77.897 kb on - strand, within PFLU_RS00390 at 77.933 kb on - strand, within PFLU_RS00390 at 77.953 kb on + strand, within PFLU_RS00390 at 77.990 kb on + strand, within PFLU_RS00390 at 77.990 kb on + strand, within PFLU_RS00390 at 77.991 kb on - strand, within PFLU_RS00390 at 77.991 kb on - strand, within PFLU_RS00390 at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.083 kb on + strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.084 kb on - strand at 78.092 kb on + strand at 78.093 kb on - strand at 78.093 kb on - strand at 78.124 kb on + strand at 78.124 kb on + strand at 78.125 kb on - strand at 78.125 kb on - strand at 78.125 kb on - strand at 78.129 kb on + strand at 78.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose (C) and Betaine (N); with MOPS; with chloride remove 76,804 + +0.4 76,804 + +1.0 76,804 + +2.0 76,804 + +1.0 76,804 + -0.1 76,804 + -0.0 76,805 - -0.1 76,805 - -1.1 76,805 - -1.2 76,805 - -0.0 77,081 + PFLU_RS00385 0.31 +1.0 77,108 + PFLU_RS00385 0.33 -0.0 77,325 + PFLU_RS00385 0.56 +2.0 77,325 + PFLU_RS00385 0.56 +0.4 77,326 - PFLU_RS00385 0.56 +2.0 77,746 + +1.0 77,759 - +0.4 77,792 + -1.9 77,793 - +0.9 77,793 - -0.1 77,793 - -0.6 77,838 - PFLU_RS00390 0.13 -0.5 77,887 - PFLU_RS00390 0.30 -1.2 77,896 + PFLU_RS00390 0.33 -0.0 77,896 + PFLU_RS00390 0.33 -1.7 77,896 + PFLU_RS00390 0.33 -1.2 77,896 + PFLU_RS00390 0.33 -1.0 77,896 + PFLU_RS00390 0.33 -1.7 77,897 - PFLU_RS00390 0.34 +1.0 77,897 - PFLU_RS00390 0.34 -0.6 77,933 - PFLU_RS00390 0.46 -1.0 77,953 + PFLU_RS00390 0.53 -2.2 77,990 + PFLU_RS00390 0.66 -3.5 77,990 + PFLU_RS00390 0.66 -1.6 77,991 - PFLU_RS00390 0.66 +1.0 77,991 - PFLU_RS00390 0.66 -1.2 78,083 + -0.6 78,083 + -2.0 78,083 + -0.6 78,083 + -0.6 78,083 + -0.3 78,083 + -0.9 78,083 + -1.9 78,083 + -0.8 78,083 + -3.0 78,083 + -0.6 78,083 + +0.4 78,083 + -1.4 78,083 + -1.8 78,083 + -1.6 78,083 + -0.8 78,083 + -0.5 78,083 + -0.9 78,083 + -1.3 78,083 + +1.1 78,083 + -0.9 78,083 + -0.6 78,083 + -1.5 78,084 - +1.5 78,084 - -1.7 78,084 - -2.8 78,084 - +2.5 78,084 - -2.1 78,084 - +0.9 78,084 - -0.6 78,084 - +0.6 78,084 - -1.0 78,084 - -0.6 78,084 - -0.5 78,084 - -0.6 78,084 - -0.5 78,084 - +0.8 78,084 - -0.3 78,084 - -0.0 78,084 - -0.5 78,092 + -1.7 78,093 - -0.0 78,093 - -0.5 78,124 + +0.4 78,124 + -1.9 78,125 - -1.6 78,125 - +2.0 78,125 - -0.0 78,129 + +2.4 78,130 - +0.6
Or see this region's nucleotide sequence