Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000805 and ABIE53_000806 are separated by 40 nucleotides ABIE53_000806 and ABIE53_000807 are separated by 10 nucleotides ABIE53_000807 and ABIE53_000808 are separated by 15 nucleotides
ABIE53_000805: ABIE53_000805 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 873,330 to 874,088
_000805
ABIE53_000806: ABIE53_000806 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase, at 874,129 to 874,683
_000806
ABIE53_000807: ABIE53_000807 - glycyl-tRNA synthetase beta chain, at 874,694 to 876,796
_000807
ABIE53_000808: ABIE53_000808 - glycyl-tRNA synthetase alpha chain, at 876,812 to 877,837
_000808
Position (kb) 874
875
876
877 Strain fitness (log2 ratio) -2
-1
0
1 at 873.776 kb on - strand, within ABIE53_000805 at 873.799 kb on + strand, within ABIE53_000805 at 873.799 kb on + strand, within ABIE53_000805 at 873.799 kb on + strand, within ABIE53_000805 at 873.800 kb on - strand, within ABIE53_000805 at 873.914 kb on - strand, within ABIE53_000805 at 874.044 kb on + strand at 874.055 kb on - strand at 874.055 kb on - strand at 874.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 873,776 - ABIE53_000805 0.59 -2.0 873,799 + ABIE53_000805 0.62 -1.8 873,799 + ABIE53_000805 0.62 -2.1 873,799 + ABIE53_000805 0.62 -1.5 873,800 - ABIE53_000805 0.62 -0.5 873,914 - ABIE53_000805 0.77 -1.6 874,044 + -1.3 874,055 - -2.1 874,055 - -2.5 874,055 - -0.7
Or see this region's nucleotide sequence