Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15355

Experiment: Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15345 and BWI76_RS15350 are separated by 149 nucleotidesBWI76_RS15350 and BWI76_RS15355 are separated by 67 nucleotidesBWI76_RS15355 and BWI76_RS15360 are separated by 423 nucleotidesBWI76_RS15360 and BWI76_RS15365 are separated by 88 nucleotides BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127 _RS15345 BWI76_RS15350: BWI76_RS15350 - hypothetical protein, at 3,215,277 to 3,215,669 _RS15350 BWI76_RS15355: BWI76_RS15355 - transcriptional regulator FtrA, at 3,215,737 to 3,216,747 _RS15355 BWI76_RS15360: BWI76_RS15360 - hypothetical protein, at 3,217,171 to 3,217,506 _RS15360 BWI76_RS15365: BWI76_RS15365 - hypothetical protein, at 3,217,595 to 3,217,927 _RS15365 Position (kb) 3215 3216 3217Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3214.844 kb on - strand, within BWI76_RS15345at 3215.104 kb on + strandat 3215.295 kb on + strandat 3215.297 kb on - strandat 3215.303 kb on - strandat 3215.354 kb on - strand, within BWI76_RS15350at 3216.042 kb on + strand, within BWI76_RS15355at 3216.052 kb on + strand, within BWI76_RS15355at 3216.220 kb on + strand, within BWI76_RS15355at 3216.228 kb on - strand, within BWI76_RS15355at 3216.510 kb on - strand, within BWI76_RS15355at 3216.603 kb on - strand, within BWI76_RS15355at 3216.604 kb on - strand, within BWI76_RS15355at 3216.609 kb on - strandat 3216.617 kb on + strandat 3216.617 kb on + strand, within BWI76_RS15355at 3216.625 kb on - strand, within BWI76_RS15355at 3216.625 kb on - strand, within BWI76_RS15355at 3216.625 kb on - strand, within BWI76_RS15355at 3216.654 kb on + strandat 3216.669 kb on - strandat 3216.727 kb on - strandat 3216.805 kb on + strandat 3216.806 kb on + strandat 3216.811 kb on + strandat 3216.825 kb on + strandat 3216.825 kb on + strandat 3216.825 kb on + strandat 3216.825 kb on + strandat 3216.825 kb on + strandat 3216.825 kb on + strandat 3216.833 kb on - strandat 3216.833 kb on - strandat 3216.833 kb on - strandat 3216.833 kb on - strandat 3216.833 kb on - strandat 3216.833 kb on - strandat 3216.834 kb on + strandat 3216.843 kb on - strandat 3216.844 kb on + strandat 3216.844 kb on + strandat 3216.844 kb on + strandat 3216.844 kb on + strandat 3216.844 kb on + strandat 3216.852 kb on - strandat 3216.875 kb on + strandat 3216.875 kb on + strandat 3216.985 kb on + strandat 3216.998 kb on + strandat 3217.026 kb on + strandat 3217.033 kb on - strandat 3217.034 kb on - strandat 3217.034 kb on - strandat 3217.080 kb on - strandat 3217.106 kb on - strandat 3217.123 kb on - strandat 3217.226 kb on + strand, within BWI76_RS15360at 3217.229 kb on - strand, within BWI76_RS15360at 3217.234 kb on - strand, within BWI76_RS15360at 3217.293 kb on + strand, within BWI76_RS15360at 3217.293 kb on + strand, within BWI76_RS15360at 3217.463 kb on + strand, within BWI76_RS15360

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
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3,214,844 - BWI76_RS15345 0.74 -0.1
3,215,104 + +0.5
3,215,295 + -0.3
3,215,297 - +0.1
3,215,303 - +1.0
3,215,354 - BWI76_RS15350 0.20 -2.4
3,216,042 + BWI76_RS15355 0.30 -0.0
3,216,052 + BWI76_RS15355 0.31 +0.3
3,216,220 + BWI76_RS15355 0.48 +1.5
3,216,228 - BWI76_RS15355 0.49 +0.3
3,216,510 - BWI76_RS15355 0.76 +0.1
3,216,603 - BWI76_RS15355 0.86 +0.6
3,216,604 - BWI76_RS15355 0.86 +0.9
3,216,609 - +0.5
3,216,617 + +1.1
3,216,617 + BWI76_RS15355 0.87 +0.4
3,216,625 - BWI76_RS15355 0.88 +0.9
3,216,625 - BWI76_RS15355 0.88 -1.2
3,216,625 - BWI76_RS15355 0.88 +0.8
3,216,654 + -1.0
3,216,669 - +0.3
3,216,727 - +0.1
3,216,805 + +1.1
3,216,806 + -2.7
3,216,811 + -2.3
3,216,825 + +0.2
3,216,825 + -1.2
3,216,825 + -0.7
3,216,825 + +2.1
3,216,825 + +0.5
3,216,825 + -1.1
3,216,833 - +0.8
3,216,833 - +3.4
3,216,833 - -3.0
3,216,833 - -0.6
3,216,833 - -1.7
3,216,833 - -2.5
3,216,834 + -0.1
3,216,843 - -1.9
3,216,844 + -0.5
3,216,844 + -0.1
3,216,844 + +2.8
3,216,844 + -0.5
3,216,844 + +1.3
3,216,852 - -1.2
3,216,875 + -0.3
3,216,875 + +1.1
3,216,985 + -0.5
3,216,998 + -0.1
3,217,026 + -0.7
3,217,033 - +0.5
3,217,034 - +1.5
3,217,034 - +0.9
3,217,080 - -0.9
3,217,106 - -1.1
3,217,123 - -2.1
3,217,226 + BWI76_RS15360 0.16 -1.9
3,217,229 - BWI76_RS15360 0.17 -1.5
3,217,234 - BWI76_RS15360 0.19 +0.1
3,217,293 + BWI76_RS15360 0.36 -2.1
3,217,293 + BWI76_RS15360 0.36 -1.5
3,217,463 + BWI76_RS15360 0.87 -1.0

Or see this region's nucleotide sequence