Experiment: MoLS4 with Formamide 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE-1 and moeB are separated by 268 nucleotides moeB and thiH overlap by 16 nucleotides thiH and thiG are separated by 2 nucleotides thiG and thiS overlap by 1 nucleotides
DVU2091: thiE-1 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,180,539 to 2,181,219
thiE-1
DVU2092: moeB - thiF family protein (TIGR), at 2,181,488 to 2,182,129
moeB
DVU2093: thiH - thiH protein (TIGR), at 2,182,114 to 2,183,448
thiH
DVU2094: thiG - thiG protein (TIGR), at 2,183,451 to 2,184,248
thiG
DVU2095: thiS - thiamine biosynthesis protein ThiS (TIGR), at 2,184,248 to 2,184,448
thiS
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2181.192 kb on + strand at 2181.236 kb on - strand at 2181.406 kb on + strand at 2181.432 kb on - strand at 2181.583 kb on + strand, within moeB at 2181.647 kb on - strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.875 kb on - strand, within moeB at 2181.934 kb on - strand, within moeB at 2182.010 kb on - strand, within moeB at 2182.083 kb on - strand at 2182.139 kb on - strand at 2182.204 kb on - strand at 2182.219 kb on - strand at 2182.258 kb on - strand, within thiH at 2182.258 kb on - strand, within thiH at 2182.278 kb on + strand, within thiH at 2182.393 kb on + strand, within thiH at 2182.401 kb on - strand, within thiH at 2182.474 kb on + strand, within thiH at 2182.853 kb on + strand, within thiH at 2182.878 kb on - strand, within thiH at 2182.887 kb on + strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.055 kb on + strand, within thiH at 2183.063 kb on + strand, within thiH at 2183.120 kb on - strand, within thiH at 2183.125 kb on - strand, within thiH at 2183.159 kb on - strand, within thiH at 2183.193 kb on - strand, within thiH at 2183.195 kb on + strand, within thiH at 2183.220 kb on - strand, within thiH at 2183.222 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.237 kb on - strand, within thiH at 2183.284 kb on + strand, within thiH at 2183.344 kb on - strand at 2183.430 kb on + strand at 2183.502 kb on + strand at 2183.524 kb on - strand at 2183.525 kb on - strand at 2183.563 kb on + strand, within thiG at 2183.569 kb on - strand, within thiG at 2183.613 kb on + strand, within thiG at 2183.647 kb on + strand, within thiG at 2183.746 kb on + strand, within thiG at 2183.836 kb on + strand, within thiG at 2183.952 kb on + strand, within thiG at 2183.983 kb on + strand, within thiG at 2184.212 kb on + strand at 2184.252 kb on - strand at 2184.282 kb on + strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.336 kb on + strand, within thiS at 2184.380 kb on + strand, within thiS at 2184.397 kb on + strand, within thiS at 2184.405 kb on - strand, within thiS
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Formamide 10mM (N) remove 2,181,192 + -2.8 2,181,236 - -0.1 2,181,406 + +0.4 2,181,432 - -0.2 2,181,583 + moeB DVU2092 0.15 -4.1 2,181,647 - moeB DVU2092 0.25 -2.5 2,181,762 + moeB DVU2092 0.43 -3.3 2,181,762 + moeB DVU2092 0.43 -4.4 2,181,875 - moeB DVU2092 0.60 -2.3 2,181,934 - moeB DVU2092 0.69 -3.3 2,182,010 - moeB DVU2092 0.81 +0.1 2,182,083 - -3.2 2,182,139 - +0.8 2,182,204 - -0.6 2,182,219 - -0.9 2,182,258 - thiH DVU2093 0.11 -1.8 2,182,258 - thiH DVU2093 0.11 -1.2 2,182,278 + thiH DVU2093 0.12 -3.3 2,182,393 + thiH DVU2093 0.21 -0.9 2,182,401 - thiH DVU2093 0.21 -1.8 2,182,474 + thiH DVU2093 0.27 -0.9 2,182,853 + thiH DVU2093 0.55 -1.1 2,182,878 - thiH DVU2093 0.57 -3.4 2,182,887 + thiH DVU2093 0.58 -3.4 2,183,038 - thiH DVU2093 0.69 -1.7 2,183,038 - thiH DVU2093 0.69 -3.1 2,183,055 + thiH DVU2093 0.70 -3.9 2,183,063 + thiH DVU2093 0.71 -3.3 2,183,120 - thiH DVU2093 0.75 -3.2 2,183,125 - thiH DVU2093 0.76 +0.7 2,183,159 - thiH DVU2093 0.78 -3.2 2,183,193 - thiH DVU2093 0.81 -2.6 2,183,195 + thiH DVU2093 0.81 -2.7 2,183,220 - thiH DVU2093 0.83 -2.3 2,183,222 + thiH DVU2093 0.83 -3.4 2,183,229 + thiH DVU2093 0.84 -2.4 2,183,229 + thiH DVU2093 0.84 -0.9 2,183,237 - thiH DVU2093 0.84 -0.6 2,183,284 + thiH DVU2093 0.88 -2.4 2,183,344 - -3.8 2,183,430 + -4.1 2,183,502 + -3.6 2,183,524 - -1.2 2,183,525 - -4.3 2,183,563 + thiG DVU2094 0.14 -3.5 2,183,569 - thiG DVU2094 0.15 +0.7 2,183,613 + thiG DVU2094 0.20 -3.1 2,183,647 + thiG DVU2094 0.25 -2.9 2,183,746 + thiG DVU2094 0.37 -0.3 2,183,836 + thiG DVU2094 0.48 -2.9 2,183,952 + thiG DVU2094 0.63 -2.9 2,183,983 + thiG DVU2094 0.67 -3.6 2,184,212 + -1.6 2,184,252 - -2.6 2,184,282 + thiS DVU2095 0.17 -3.3 2,184,290 - thiS DVU2095 0.21 -1.9 2,184,290 - thiS DVU2095 0.21 -1.2 2,184,336 + thiS DVU2095 0.44 -0.6 2,184,380 + thiS DVU2095 0.66 -2.6 2,184,397 + thiS DVU2095 0.74 -3.2 2,184,405 - thiS DVU2095 0.78 -3.5
Or see this region's nucleotide sequence