Experiment: MoLS4 with Formamide 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrf2 and rrf1 are separated by 3 nucleotides rrf1 and hmcF are separated by 92 nucleotides hmcF and hmcE are separated by 23 nucleotides hmcE and hmcD are separated by 21 nucleotides hmcD and hmcC are separated by 15 nucleotides
DVU0529: rrf2 - Rrf2 protein (voordouw), at 601,638 to 602,090
rrf2
DVU0530: rrf1 - Rrf1 protein (voordouw), at 602,094 to 602,510
rrf1
DVU0531: hmcF - HmcF, 52.7 kd protein in hmc operon (voordouw), at 602,603 to 603,988
hmcF
DVU0532: hmcE - HmcE, 25.3 kd protein in hmc operon (voordouw), at 604,012 to 604,692
hmcE
DVU0533: hmcD - HmcD, 5.8 kd protein in hmc operon (voordouw), at 604,714 to 604,857
hmcD
DVU0534: hmcC - HmcC, 43.2 kd protein in hmc operon (voordouw), at 604,873 to 606,039
hmcC
Position (kb) 602
603
604 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 601.629 kb on + strand at 601.636 kb on + strand at 601.776 kb on + strand, within rrf2 at 601.791 kb on - strand, within rrf2 at 601.851 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.936 kb on + strand, within rrf2 at 602.011 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.085 kb on + strand at 602.087 kb on - strand at 602.143 kb on - strand, within rrf1 at 602.200 kb on - strand, within rrf1 at 602.259 kb on + strand, within rrf1 at 602.353 kb on - strand, within rrf1 at 602.398 kb on - strand, within rrf1 at 602.425 kb on + strand, within rrf1 at 602.440 kb on + strand, within rrf1 at 602.448 kb on - strand, within rrf1 at 602.508 kb on + strand at 602.518 kb on - strand at 602.524 kb on - strand at 602.624 kb on - strand at 602.726 kb on + strand at 602.804 kb on - strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.090 kb on - strand, within hmcF at 603.119 kb on + strand, within hmcF at 603.125 kb on + strand, within hmcF at 603.170 kb on - strand, within hmcF at 603.273 kb on + strand, within hmcF at 603.273 kb on + strand, within hmcF at 603.281 kb on - strand, within hmcF at 603.360 kb on + strand, within hmcF at 603.443 kb on + strand, within hmcF at 603.502 kb on - strand, within hmcF at 603.534 kb on - strand, within hmcF at 603.540 kb on + strand, within hmcF at 603.548 kb on - strand, within hmcF at 603.599 kb on + strand, within hmcF at 603.602 kb on - strand, within hmcF at 603.602 kb on - strand, within hmcF at 603.623 kb on + strand, within hmcF at 603.651 kb on - strand, within hmcF at 603.651 kb on - strand, within hmcF at 603.677 kb on - strand, within hmcF at 603.767 kb on - strand, within hmcF at 603.894 kb on + strand at 603.928 kb on - strand at 603.930 kb on + strand at 603.930 kb on + strand at 603.938 kb on - strand at 604.026 kb on - strand at 604.058 kb on + strand at 604.141 kb on - strand, within hmcE at 604.149 kb on - strand, within hmcE at 604.151 kb on - strand, within hmcE at 604.282 kb on + strand, within hmcE at 604.327 kb on + strand, within hmcE at 604.363 kb on - strand, within hmcE at 604.363 kb on - strand, within hmcE at 604.499 kb on + strand, within hmcE at 604.632 kb on - strand at 604.694 kb on + strand at 604.694 kb on + strand at 604.774 kb on - strand, within hmcD at 604.779 kb on + strand, within hmcD at 604.787 kb on - strand, within hmcD at 604.846 kb on - strand at 604.868 kb on + strand at 604.883 kb on + strand at 604.988 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Formamide 10mM (N) remove 601,629 + +1.3 601,636 + +0.8 601,776 + rrf2 DVU0529 0.30 -1.0 601,791 - rrf2 DVU0529 0.34 +0.3 601,851 + rrf2 DVU0529 0.47 -0.1 601,872 + rrf2 DVU0529 0.52 -1.2 601,872 + rrf2 DVU0529 0.52 +0.5 601,936 + rrf2 DVU0529 0.66 -0.8 602,011 + rrf2 DVU0529 0.82 -0.6 602,021 + rrf2 DVU0529 0.85 +0.3 602,021 + rrf2 DVU0529 0.85 -0.7 602,021 + rrf2 DVU0529 0.85 -1.7 602,085 + -0.6 602,087 - -1.0 602,143 - rrf1 DVU0530 0.12 -0.2 602,200 - rrf1 DVU0530 0.25 -1.5 602,259 + rrf1 DVU0530 0.40 +1.0 602,353 - rrf1 DVU0530 0.62 +2.3 602,398 - rrf1 DVU0530 0.73 +0.2 602,425 + rrf1 DVU0530 0.79 +0.7 602,440 + rrf1 DVU0530 0.83 -0.2 602,448 - rrf1 DVU0530 0.85 +0.6 602,508 + +0.1 602,518 - -0.2 602,524 - +0.9 602,624 - -1.4 602,726 + -0.3 602,804 - hmcF DVU0531 0.15 -0.7 603,048 + hmcF DVU0531 0.32 +0.8 603,048 + hmcF DVU0531 0.32 -0.9 603,090 - hmcF DVU0531 0.35 -1.0 603,119 + hmcF DVU0531 0.37 -0.6 603,125 + hmcF DVU0531 0.38 -0.7 603,170 - hmcF DVU0531 0.41 -0.7 603,273 + hmcF DVU0531 0.48 -1.0 603,273 + hmcF DVU0531 0.48 -0.8 603,281 - hmcF DVU0531 0.49 -0.1 603,360 + hmcF DVU0531 0.55 -0.3 603,443 + hmcF DVU0531 0.61 -2.7 603,502 - hmcF DVU0531 0.65 -0.2 603,534 - hmcF DVU0531 0.67 -0.9 603,540 + hmcF DVU0531 0.68 -1.0 603,548 - hmcF DVU0531 0.68 -0.8 603,599 + hmcF DVU0531 0.72 -2.5 603,602 - hmcF DVU0531 0.72 -0.7 603,602 - hmcF DVU0531 0.72 -1.6 603,623 + hmcF DVU0531 0.74 -3.1 603,651 - hmcF DVU0531 0.76 -1.7 603,651 - hmcF DVU0531 0.76 -0.6 603,677 - hmcF DVU0531 0.77 -2.1 603,767 - hmcF DVU0531 0.84 -1.2 603,894 + -1.2 603,928 - -1.6 603,930 + -2.1 603,930 + -1.5 603,938 - -1.2 604,026 - -0.3 604,058 + +2.3 604,141 - hmcE DVU0532 0.19 -0.9 604,149 - hmcE DVU0532 0.20 +0.6 604,151 - hmcE DVU0532 0.20 +0.3 604,282 + hmcE DVU0532 0.40 -0.9 604,327 + hmcE DVU0532 0.46 +1.3 604,363 - hmcE DVU0532 0.52 -1.2 604,363 - hmcE DVU0532 0.52 -0.4 604,499 + hmcE DVU0532 0.72 -0.2 604,632 - -1.1 604,694 + -0.8 604,694 + -0.9 604,774 - hmcD DVU0533 0.42 +0.3 604,779 + hmcD DVU0533 0.45 +0.3 604,787 - hmcD DVU0533 0.51 -0.7 604,846 - -1.8 604,868 + -0.8 604,883 + -0.6 604,988 - -1.6
Or see this region's nucleotide sequence