Experiment: Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26210 and BWI76_RS26215 overlap by 4 nucleotides BWI76_RS26215 and BWI76_RS26220 are separated by 247 nucleotides
BWI76_RS26210: BWI76_RS26210 - glycogen debranching enzyme, at 5,422,264 to 5,424,240
_RS26210
BWI76_RS26215: BWI76_RS26215 - glycogen-branching enzyme, at 5,424,237 to 5,426,423
_RS26215
BWI76_RS26220: BWI76_RS26220 - aspartate-semialdehyde dehydrogenase, at 5,426,671 to 5,427,777
_RS26220
Position (kb)
5424
5425
5426
5427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5423.454 kb on - strand, within BWI76_RS26210 at 5423.763 kb on - strand, within BWI76_RS26210 at 5423.770 kb on - strand, within BWI76_RS26210 at 5423.802 kb on - strand, within BWI76_RS26210 at 5423.915 kb on + strand, within BWI76_RS26210 at 5423.978 kb on - strand, within BWI76_RS26210 at 5424.138 kb on + strand at 5424.183 kb on - strand at 5424.345 kb on - strand at 5424.504 kb on + strand, within BWI76_RS26215 at 5424.604 kb on - strand, within BWI76_RS26215 at 5424.652 kb on + strand, within BWI76_RS26215 at 5424.675 kb on + strand, within BWI76_RS26215 at 5424.786 kb on + strand, within BWI76_RS26215 at 5425.243 kb on + strand, within BWI76_RS26215 at 5425.243 kb on + strand, within BWI76_RS26215 at 5425.251 kb on - strand, within BWI76_RS26215 at 5425.317 kb on + strand, within BWI76_RS26215 at 5425.356 kb on - strand, within BWI76_RS26215 at 5425.578 kb on + strand, within BWI76_RS26215 at 5425.943 kb on + strand, within BWI76_RS26215 at 5426.125 kb on - strand, within BWI76_RS26215 at 5426.334 kb on + strand at 5426.413 kb on + strand at 5426.489 kb on - strand at 5426.501 kb on - strand at 5426.513 kb on - strand at 5426.535 kb on - strand at 5426.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM remove 5,423,454 - BWI76_RS26210 0.60 -0.0 5,423,763 - BWI76_RS26210 0.76 -0.3 5,423,770 - BWI76_RS26210 0.76 -0.2 5,423,802 - BWI76_RS26210 0.78 -0.3 5,423,915 + BWI76_RS26210 0.84 +0.7 5,423,978 - BWI76_RS26210 0.87 +0.4 5,424,138 + +1.0 5,424,183 - +0.9 5,424,345 - +0.4 5,424,504 + BWI76_RS26215 0.12 +0.8 5,424,604 - BWI76_RS26215 0.17 +1.6 5,424,652 + BWI76_RS26215 0.19 -0.7 5,424,675 + BWI76_RS26215 0.20 +1.0 5,424,786 + BWI76_RS26215 0.25 -1.8 5,425,243 + BWI76_RS26215 0.46 +1.0 5,425,243 + BWI76_RS26215 0.46 -0.6 5,425,251 - BWI76_RS26215 0.46 +0.4 5,425,317 + BWI76_RS26215 0.49 +2.4 5,425,356 - BWI76_RS26215 0.51 -0.4 5,425,578 + BWI76_RS26215 0.61 +1.0 5,425,943 + BWI76_RS26215 0.78 +2.0 5,426,125 - BWI76_RS26215 0.86 +2.7 5,426,334 + +1.3 5,426,413 + +1.0 5,426,489 - -3.1 5,426,501 - +0.0 5,426,513 - +0.5 5,426,535 - +2.2 5,426,572 + +1.2
Or see this region's nucleotide sequence