Experiment: Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21145 and BWI76_RS21150 are separated by 73 nucleotides BWI76_RS21150 and BWI76_RS21155 are separated by 19 nucleotides BWI76_RS21155 and BWI76_RS21160 are separated by 26 nucleotides BWI76_RS21160 and BWI76_RS21165 are separated by 177 nucleotides BWI76_RS21165 and BWI76_RS21170 are separated by 233 nucleotides
BWI76_RS21145: BWI76_RS21145 - co-chaperone HscB, at 4,428,711 to 4,429,226
_RS21145
BWI76_RS21150: BWI76_RS21150 - iron-sulfur cluster assembly accessory protein, at 4,429,300 to 4,429,623
_RS21150
BWI76_RS21155: BWI76_RS21155 - iron-sulfur cluster scaffold-like protein, at 4,429,643 to 4,430,029
_RS21155
BWI76_RS21160: BWI76_RS21160 - IscS subfamily cysteine desulfurase, at 4,430,056 to 4,431,270
_RS21160
BWI76_RS21165: BWI76_RS21165 - Fe-S cluster assembly transcriptional regulator IscR, at 4,431,448 to 4,431,939
_RS21165
BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904
_RS21170
Position (kb)
4430
4431
4432 Strain fitness (log2 ratio)
-2
-1
0
1 at 4429.252 kb on + strand at 4429.284 kb on + strand at 4429.713 kb on - strand, within BWI76_RS21155 at 4431.572 kb on - strand, within BWI76_RS21165 at 4432.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM remove 4,429,252 + +0.0 4,429,284 + -0.6 4,429,713 - BWI76_RS21155 0.18 +1.0 4,431,572 - BWI76_RS21165 0.25 -2.5 4,432,156 + +0.0
Or see this region's nucleotide sequence