Experiment: MoLS4 with Formamide 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvB-2 and ilvN-2 are separated by 9 nucleotides ilvN-2 and ilvC are separated by 121 nucleotides ilvC and DVU1380 are separated by 262 nucleotides DVU1380 and DVU1381 overlap by 20 nucleotides DVU1381 and DVU1382 are separated by 60 nucleotides
DVU1376: ilvB-2 - acetolactate synthase, large subunit, biosynthetic type (TIGR), at 1,451,544 to 1,453,235
ilvB-2
DVU1377: ilvN-2 - acetolactate synthase, small subunit (TIGR), at 1,453,245 to 1,453,733
ilvN-2
DVU1378: ilvC - ketol-acid reductoisomerase (TIGR), at 1,453,855 to 1,454,850
ilvC
DVU1380: DVU1380 - hypothetical protein (TIGR), at 1,455,113 to 1,455,343
DVU1380
DVU1381: DVU1381 - hypothetical protein (TIGR), at 1,455,324 to 1,455,539
DVU1381
DVU1382: DVU1382 - HesB family selenoprotein (TIGR), at 1,455,600 to 1,455,923
DVU1382
Position (kb)
1453
1454
1455 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1453.110 kb on - strand at 1453.158 kb on - strand at 1453.247 kb on - strand at 1453.249 kb on + strand at 1453.376 kb on + strand, within ilvN-2 at 1453.486 kb on + strand, within ilvN-2 at 1453.696 kb on + strand at 1453.701 kb on + strand at 1454.150 kb on - strand, within ilvC at 1454.208 kb on + strand, within ilvC at 1454.305 kb on - strand, within ilvC at 1454.352 kb on - strand, within ilvC at 1454.355 kb on + strand, within ilvC at 1454.378 kb on + strand, within ilvC at 1454.418 kb on - strand, within ilvC at 1454.420 kb on - strand, within ilvC at 1454.420 kb on - strand, within ilvC at 1454.477 kb on - strand, within ilvC at 1454.600 kb on - strand, within ilvC at 1454.711 kb on - strand, within ilvC at 1454.827 kb on - strand at 1454.989 kb on - strand at 1455.031 kb on + strand at 1455.066 kb on + strand at 1455.094 kb on + strand at 1455.101 kb on + strand at 1455.134 kb on - strand at 1455.205 kb on - strand, within DVU1380 at 1455.246 kb on + strand, within DVU1380 at 1455.305 kb on + strand, within DVU1380
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Formamide 10mM (N) remove 1,453,110 - -5.7 1,453,158 - -3.9 1,453,247 - -1.7 1,453,249 + -2.0 1,453,376 + ilvN-2 DVU1377 0.27 -0.9 1,453,486 + ilvN-2 DVU1377 0.49 -0.7 1,453,696 + -0.1 1,453,701 + -1.8 1,454,150 - ilvC DVU1378 0.30 -5.8 1,454,208 + ilvC DVU1378 0.35 -5.6 1,454,305 - ilvC DVU1378 0.45 -4.9 1,454,352 - ilvC DVU1378 0.50 -3.0 1,454,355 + ilvC DVU1378 0.50 -0.1 1,454,378 + ilvC DVU1378 0.53 -5.9 1,454,418 - ilvC DVU1378 0.57 -1.1 1,454,420 - ilvC DVU1378 0.57 -5.8 1,454,420 - ilvC DVU1378 0.57 -6.0 1,454,477 - ilvC DVU1378 0.62 -1.1 1,454,600 - ilvC DVU1378 0.75 -1.1 1,454,711 - ilvC DVU1378 0.86 -5.2 1,454,827 - -2.1 1,454,989 - -0.0 1,455,031 + -0.7 1,455,066 + -0.4 1,455,094 + +0.0 1,455,101 + -0.2 1,455,134 - -3.0 1,455,205 - DVU1380 0.40 -0.4 1,455,246 + DVU1380 0.58 -1.0 1,455,305 + DVU1380 0.83 -0.2
Or see this region's nucleotide sequence