Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS23435 and ABZR87_RS23440 overlap by 1 nucleotides ABZR87_RS23440 and ABZR87_RS23445 overlap by 14 nucleotides ABZR87_RS23445 and ABZR87_RS23450 are separated by 9 nucleotides ABZR87_RS23450 and ABZR87_RS23455 overlap by 4 nucleotides
ABZR87_RS23435: ABZR87_RS23435 - malonate decarboxylase subunit alpha, at 1,621,954 to 1,623,642
_RS23435
ABZR87_RS23440: ABZR87_RS23440 - triphosphoribosyl-dephospho-CoA synthase, at 1,623,642 to 1,624,571
_RS23440
ABZR87_RS23445: ABZR87_RS23445 - malonate decarboxylase subunit delta, at 1,624,558 to 1,624,863
_RS23445
ABZR87_RS23450: ABZR87_RS23450 - biotin-independent malonate decarboxylase subunit beta, at 1,624,873 to 1,625,718
_RS23450
ABZR87_RS23455: ABZR87_RS23455 - biotin-independent malonate decarboxylase subunit gamma, at 1,625,715 to 1,626,545
_RS23455
Position (kb)
1624
1625 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1623.610 kb on + strand at 1623.680 kb on + strand at 1623.680 kb on + strand at 1623.681 kb on - strand at 1624.043 kb on - strand, within ABZR87_RS23440 at 1624.147 kb on - strand, within ABZR87_RS23440 at 1624.612 kb on + strand, within ABZR87_RS23445 at 1624.612 kb on + strand, within ABZR87_RS23445 at 1624.613 kb on - strand, within ABZR87_RS23445 at 1624.861 kb on + strand at 1624.862 kb on - strand at 1625.022 kb on + strand, within ABZR87_RS23450 at 1625.380 kb on + strand, within ABZR87_RS23450 at 1625.380 kb on + strand, within ABZR87_RS23450 at 1625.380 kb on + strand, within ABZR87_RS23450 at 1625.458 kb on + strand, within ABZR87_RS23450 at 1625.458 kb on + strand, within ABZR87_RS23450 at 1625.459 kb on - strand, within ABZR87_RS23450 at 1625.633 kb on - strand, within ABZR87_RS23450 at 1625.633 kb on - strand, within ABZR87_RS23450 at 1625.633 kb on - strand, within ABZR87_RS23450 at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.687 kb on + strand at 1625.698 kb on + strand at 1625.699 kb on - strand at 1625.699 kb on - strand at 1625.699 kb on - strand at 1625.844 kb on + strand, within ABZR87_RS23455 at 1625.845 kb on - strand, within ABZR87_RS23455
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,623,610 + -0.5 1,623,680 + -0.0 1,623,680 + -1.1 1,623,681 - -0.2 1,624,043 - ABZR87_RS23440 0.43 -0.3 1,624,147 - ABZR87_RS23440 0.54 -0.5 1,624,612 + ABZR87_RS23445 0.18 -0.8 1,624,612 + ABZR87_RS23445 0.18 -0.9 1,624,613 - ABZR87_RS23445 0.18 -0.4 1,624,861 + -0.3 1,624,862 - -1.0 1,625,022 + ABZR87_RS23450 0.18 -0.9 1,625,380 + ABZR87_RS23450 0.60 +0.7 1,625,380 + ABZR87_RS23450 0.60 -0.3 1,625,380 + ABZR87_RS23450 0.60 -2.3 1,625,458 + ABZR87_RS23450 0.69 -0.2 1,625,458 + ABZR87_RS23450 0.69 -0.0 1,625,459 - ABZR87_RS23450 0.69 -1.4 1,625,633 - ABZR87_RS23450 0.90 -1.3 1,625,633 - ABZR87_RS23450 0.90 -1.5 1,625,633 - ABZR87_RS23450 0.90 +0.6 1,625,687 + -0.5 1,625,687 + -0.9 1,625,687 + -0.4 1,625,687 + -1.5 1,625,687 + +0.0 1,625,687 + +1.6 1,625,687 + +1.8 1,625,698 + +1.1 1,625,699 - +0.4 1,625,699 - +1.1 1,625,699 - +0.2 1,625,844 + ABZR87_RS23455 0.16 -1.5 1,625,845 - ABZR87_RS23455 0.16 -0.4
Or see this region's nucleotide sequence