Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22725 and ABZR87_RS22730 are separated by 99 nucleotides ABZR87_RS22730 and ABZR87_RS22735 are separated by 118 nucleotides
ABZR87_RS22725: ABZR87_RS22725 - aldo/keto reductase family oxidoreductase, at 1,457,613 to 1,458,497
_RS22725
ABZR87_RS22730: ABZR87_RS22730 - LysR family transcriptional regulator, at 1,458,597 to 1,459,472
_RS22730
ABZR87_RS22735: ABZR87_RS22735 - succinylglutamate desuccinylase/aspartoacylase family protein, at 1,459,591 to 1,460,607
_RS22735
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1457.676 kb on + strand at 1457.677 kb on - strand at 1457.783 kb on - strand, within ABZR87_RS22725 at 1457.794 kb on - strand, within ABZR87_RS22725 at 1457.808 kb on + strand, within ABZR87_RS22725 at 1457.808 kb on + strand, within ABZR87_RS22725 at 1457.809 kb on - strand, within ABZR87_RS22725 at 1457.809 kb on - strand, within ABZR87_RS22725 at 1457.809 kb on - strand, within ABZR87_RS22725 at 1457.809 kb on - strand, within ABZR87_RS22725 at 1458.095 kb on + strand, within ABZR87_RS22725 at 1458.174 kb on + strand, within ABZR87_RS22725 at 1458.174 kb on + strand, within ABZR87_RS22725 at 1458.175 kb on - strand, within ABZR87_RS22725 at 1458.237 kb on + strand, within ABZR87_RS22725 at 1458.237 kb on + strand, within ABZR87_RS22725 at 1458.237 kb on + strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.238 kb on - strand, within ABZR87_RS22725 at 1458.247 kb on - strand, within ABZR87_RS22725 at 1458.562 kb on + strand at 1458.562 kb on + strand at 1458.562 kb on + strand at 1458.562 kb on + strand at 1458.619 kb on - strand at 1458.786 kb on + strand, within ABZR87_RS22730 at 1458.786 kb on + strand, within ABZR87_RS22730 at 1458.786 kb on + strand, within ABZR87_RS22730 at 1458.786 kb on + strand, within ABZR87_RS22730 at 1458.786 kb on + strand, within ABZR87_RS22730 at 1458.787 kb on - strand, within ABZR87_RS22730 at 1458.983 kb on + strand, within ABZR87_RS22730 at 1458.984 kb on - strand, within ABZR87_RS22730 at 1459.207 kb on - strand, within ABZR87_RS22730 at 1459.210 kb on - strand, within ABZR87_RS22730 at 1459.210 kb on - strand, within ABZR87_RS22730 at 1459.210 kb on - strand, within ABZR87_RS22730 at 1459.300 kb on + strand, within ABZR87_RS22730 at 1459.300 kb on + strand, within ABZR87_RS22730 at 1459.300 kb on + strand, within ABZR87_RS22730 at 1459.301 kb on - strand, within ABZR87_RS22730 at 1459.301 kb on - strand, within ABZR87_RS22730 at 1459.301 kb on - strand, within ABZR87_RS22730 at 1459.412 kb on - strand at 1459.455 kb on - strand at 1459.470 kb on + strand at 1459.701 kb on + strand, within ABZR87_RS22735 at 1459.701 kb on + strand, within ABZR87_RS22735 at 1459.702 kb on - strand, within ABZR87_RS22735 at 1459.786 kb on + strand, within ABZR87_RS22735 at 1459.786 kb on + strand, within ABZR87_RS22735 at 1459.787 kb on - strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.984 kb on + strand, within ABZR87_RS22735 at 1459.985 kb on - strand, within ABZR87_RS22735 at 1460.380 kb on + strand, within ABZR87_RS22735 at 1460.381 kb on - strand, within ABZR87_RS22735 at 1460.381 kb on - strand, within ABZR87_RS22735 at 1460.386 kb on + strand, within ABZR87_RS22735 at 1460.386 kb on + strand, within ABZR87_RS22735 at 1460.386 kb on + strand, within ABZR87_RS22735 at 1460.386 kb on + strand, within ABZR87_RS22735 at 1460.387 kb on - strand, within ABZR87_RS22735 at 1460.387 kb on - strand, within ABZR87_RS22735
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,457,676 + -0.3 1,457,677 - +0.0 1,457,783 - ABZR87_RS22725 0.19 +0.4 1,457,794 - ABZR87_RS22725 0.20 +0.2 1,457,808 + ABZR87_RS22725 0.22 +1.1 1,457,808 + ABZR87_RS22725 0.22 +0.6 1,457,809 - ABZR87_RS22725 0.22 +0.7 1,457,809 - ABZR87_RS22725 0.22 -0.9 1,457,809 - ABZR87_RS22725 0.22 +0.4 1,457,809 - ABZR87_RS22725 0.22 +0.7 1,458,095 + ABZR87_RS22725 0.54 +1.1 1,458,174 + ABZR87_RS22725 0.63 -1.1 1,458,174 + ABZR87_RS22725 0.63 -0.4 1,458,175 - ABZR87_RS22725 0.64 +0.1 1,458,237 + ABZR87_RS22725 0.71 +0.7 1,458,237 + ABZR87_RS22725 0.71 -0.5 1,458,237 + ABZR87_RS22725 0.71 -1.5 1,458,238 - ABZR87_RS22725 0.71 +0.2 1,458,238 - ABZR87_RS22725 0.71 +0.3 1,458,238 - ABZR87_RS22725 0.71 +0.1 1,458,238 - ABZR87_RS22725 0.71 +1.0 1,458,238 - ABZR87_RS22725 0.71 +0.9 1,458,238 - ABZR87_RS22725 0.71 -0.0 1,458,238 - ABZR87_RS22725 0.71 +0.4 1,458,247 - ABZR87_RS22725 0.72 +0.5 1,458,562 + -0.1 1,458,562 + -1.0 1,458,562 + +0.5 1,458,562 + -0.8 1,458,619 - -0.1 1,458,786 + ABZR87_RS22730 0.22 -0.1 1,458,786 + ABZR87_RS22730 0.22 -0.2 1,458,786 + ABZR87_RS22730 0.22 +0.3 1,458,786 + ABZR87_RS22730 0.22 +0.5 1,458,786 + ABZR87_RS22730 0.22 -0.2 1,458,787 - ABZR87_RS22730 0.22 -0.1 1,458,983 + ABZR87_RS22730 0.44 -0.2 1,458,984 - ABZR87_RS22730 0.44 -0.9 1,459,207 - ABZR87_RS22730 0.70 -0.3 1,459,210 - ABZR87_RS22730 0.70 -0.1 1,459,210 - ABZR87_RS22730 0.70 +1.1 1,459,210 - ABZR87_RS22730 0.70 +0.5 1,459,300 + ABZR87_RS22730 0.80 -0.9 1,459,300 + ABZR87_RS22730 0.80 +0.2 1,459,300 + ABZR87_RS22730 0.80 +0.5 1,459,301 - ABZR87_RS22730 0.80 +0.2 1,459,301 - ABZR87_RS22730 0.80 +0.5 1,459,301 - ABZR87_RS22730 0.80 +0.7 1,459,412 - -0.1 1,459,455 - -0.9 1,459,470 + +0.7 1,459,701 + ABZR87_RS22735 0.11 +0.8 1,459,701 + ABZR87_RS22735 0.11 +0.8 1,459,702 - ABZR87_RS22735 0.11 -0.6 1,459,786 + ABZR87_RS22735 0.19 +0.4 1,459,786 + ABZR87_RS22735 0.19 -0.7 1,459,787 - ABZR87_RS22735 0.19 -0.4 1,459,984 + ABZR87_RS22735 0.39 +0.0 1,459,984 + ABZR87_RS22735 0.39 -2.7 1,459,984 + ABZR87_RS22735 0.39 +0.1 1,459,984 + ABZR87_RS22735 0.39 +2.8 1,459,984 + ABZR87_RS22735 0.39 +0.2 1,459,984 + ABZR87_RS22735 0.39 -0.4 1,459,985 - ABZR87_RS22735 0.39 -0.1 1,460,380 + ABZR87_RS22735 0.78 +0.1 1,460,381 - ABZR87_RS22735 0.78 -0.3 1,460,381 - ABZR87_RS22735 0.78 -1.1 1,460,386 + ABZR87_RS22735 0.78 +0.4 1,460,386 + ABZR87_RS22735 0.78 +0.0 1,460,386 + ABZR87_RS22735 0.78 +0.8 1,460,386 + ABZR87_RS22735 0.78 +0.2 1,460,387 - ABZR87_RS22735 0.78 -0.7 1,460,387 - ABZR87_RS22735 0.78 -0.4
Or see this region's nucleotide sequence