Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22365 and ABZR87_RS22370 are separated by 127 nucleotides ABZR87_RS22370 and ABZR87_RS22375 are separated by 16 nucleotides ABZR87_RS22375 and ABZR87_RS22380 are separated by 88 nucleotides
ABZR87_RS22365: ABZR87_RS22365 - 2-isopropylmalate synthase, at 1,376,686 to 1,378,398
_RS22365
ABZR87_RS22370: ABZR87_RS22370 - four-helix bundle copper-binding protein, at 1,378,526 to 1,378,912
_RS22370
ABZR87_RS22375: ABZR87_RS22375 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 1,378,929 to 1,379,405
_RS22375
ABZR87_RS22380: ABZR87_RS22380 - DUF4139 domain-containing protein, at 1,379,494 to 1,381,125
_RS22380
Position (kb)
1378
1379 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1377.556 kb on + strand, within ABZR87_RS22365 at 1377.557 kb on - strand, within ABZR87_RS22365 at 1377.557 kb on - strand, within ABZR87_RS22365 at 1377.658 kb on + strand, within ABZR87_RS22365 at 1377.658 kb on + strand, within ABZR87_RS22365 at 1377.658 kb on + strand, within ABZR87_RS22365 at 1377.658 kb on + strand, within ABZR87_RS22365 at 1377.659 kb on - strand, within ABZR87_RS22365 at 1377.659 kb on - strand, within ABZR87_RS22365 at 1377.659 kb on - strand, within ABZR87_RS22365 at 1377.659 kb on - strand, within ABZR87_RS22365 at 1377.712 kb on + strand, within ABZR87_RS22365 at 1377.760 kb on + strand, within ABZR87_RS22365 at 1377.761 kb on - strand, within ABZR87_RS22365 at 1377.796 kb on + strand, within ABZR87_RS22365 at 1377.796 kb on + strand, within ABZR87_RS22365 at 1377.796 kb on + strand, within ABZR87_RS22365 at 1377.796 kb on + strand, within ABZR87_RS22365 at 1377.797 kb on - strand, within ABZR87_RS22365 at 1377.797 kb on - strand, within ABZR87_RS22365 at 1377.797 kb on - strand, within ABZR87_RS22365 at 1377.797 kb on - strand, within ABZR87_RS22365 at 1377.848 kb on - strand, within ABZR87_RS22365 at 1377.866 kb on - strand, within ABZR87_RS22365 at 1377.985 kb on + strand, within ABZR87_RS22365 at 1377.985 kb on + strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1377.986 kb on - strand, within ABZR87_RS22365 at 1378.013 kb on - strand, within ABZR87_RS22365 at 1378.013 kb on - strand, within ABZR87_RS22365 at 1378.016 kb on + strand, within ABZR87_RS22365 at 1378.016 kb on + strand, within ABZR87_RS22365 at 1378.016 kb on + strand, within ABZR87_RS22365 at 1378.016 kb on + strand, within ABZR87_RS22365 at 1378.016 kb on + strand, within ABZR87_RS22365 at 1378.017 kb on - strand, within ABZR87_RS22365 at 1378.017 kb on - strand, within ABZR87_RS22365 at 1378.017 kb on - strand, within ABZR87_RS22365 at 1378.017 kb on - strand, within ABZR87_RS22365 at 1378.180 kb on + strand, within ABZR87_RS22365 at 1378.180 kb on + strand, within ABZR87_RS22365 at 1378.394 kb on + strand at 1378.394 kb on + strand at 1378.394 kb on + strand at 1378.396 kb on + strand at 1378.396 kb on + strand at 1378.396 kb on + strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.397 kb on - strand at 1378.436 kb on + strand at 1378.436 kb on + strand at 1378.436 kb on + strand at 1378.437 kb on - strand at 1378.678 kb on + strand, within ABZR87_RS22370 at 1378.694 kb on + strand, within ABZR87_RS22370 at 1378.929 kb on - strand at 1378.986 kb on + strand, within ABZR87_RS22375 at 1378.986 kb on + strand, within ABZR87_RS22375 at 1378.986 kb on + strand, within ABZR87_RS22375 at 1378.987 kb on - strand, within ABZR87_RS22375 at 1378.987 kb on - strand, within ABZR87_RS22375 at 1378.987 kb on - strand, within ABZR87_RS22375 at 1379.034 kb on + strand, within ABZR87_RS22375 at 1379.034 kb on + strand, within ABZR87_RS22375 at 1379.034 kb on + strand, within ABZR87_RS22375 at 1379.034 kb on + strand, within ABZR87_RS22375 at 1379.035 kb on - strand, within ABZR87_RS22375 at 1379.632 kb on + strand at 1379.632 kb on + strand at 1379.633 kb on - strand at 1379.633 kb on - strand at 1379.633 kb on - strand at 1379.690 kb on + strand, within ABZR87_RS22380 at 1379.690 kb on + strand, within ABZR87_RS22380 at 1379.690 kb on + strand, within ABZR87_RS22380 at 1379.691 kb on - strand, within ABZR87_RS22380 at 1379.691 kb on - strand, within ABZR87_RS22380 at 1379.902 kb on + strand, within ABZR87_RS22380 at 1379.902 kb on + strand, within ABZR87_RS22380 at 1379.902 kb on + strand, within ABZR87_RS22380 at 1379.902 kb on + strand, within ABZR87_RS22380 at 1379.903 kb on - strand, within ABZR87_RS22380
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,377,556 + ABZR87_RS22365 0.51 -1.1 1,377,557 - ABZR87_RS22365 0.51 -0.7 1,377,557 - ABZR87_RS22365 0.51 +0.2 1,377,658 + ABZR87_RS22365 0.57 +1.5 1,377,658 + ABZR87_RS22365 0.57 +0.2 1,377,658 + ABZR87_RS22365 0.57 -1.3 1,377,658 + ABZR87_RS22365 0.57 -2.3 1,377,659 - ABZR87_RS22365 0.57 +0.3 1,377,659 - ABZR87_RS22365 0.57 +0.3 1,377,659 - ABZR87_RS22365 0.57 +0.8 1,377,659 - ABZR87_RS22365 0.57 -0.2 1,377,712 + ABZR87_RS22365 0.60 -0.0 1,377,760 + ABZR87_RS22365 0.63 -0.5 1,377,761 - ABZR87_RS22365 0.63 +0.3 1,377,796 + ABZR87_RS22365 0.65 -0.6 1,377,796 + ABZR87_RS22365 0.65 -0.6 1,377,796 + ABZR87_RS22365 0.65 +0.4 1,377,796 + ABZR87_RS22365 0.65 -0.4 1,377,797 - ABZR87_RS22365 0.65 -1.1 1,377,797 - ABZR87_RS22365 0.65 -0.6 1,377,797 - ABZR87_RS22365 0.65 -0.1 1,377,797 - ABZR87_RS22365 0.65 -0.6 1,377,848 - ABZR87_RS22365 0.68 -0.1 1,377,866 - ABZR87_RS22365 0.69 -0.5 1,377,985 + ABZR87_RS22365 0.76 -1.1 1,377,985 + ABZR87_RS22365 0.76 -0.6 1,377,986 - ABZR87_RS22365 0.76 +0.7 1,377,986 - ABZR87_RS22365 0.76 +0.2 1,377,986 - ABZR87_RS22365 0.76 -1.0 1,377,986 - ABZR87_RS22365 0.76 +0.2 1,377,986 - ABZR87_RS22365 0.76 +0.5 1,377,986 - ABZR87_RS22365 0.76 +0.0 1,377,986 - ABZR87_RS22365 0.76 -0.5 1,377,986 - ABZR87_RS22365 0.76 -0.2 1,378,013 - ABZR87_RS22365 0.77 +0.1 1,378,013 - ABZR87_RS22365 0.77 -0.7 1,378,016 + ABZR87_RS22365 0.78 +0.8 1,378,016 + ABZR87_RS22365 0.78 +0.1 1,378,016 + ABZR87_RS22365 0.78 +0.8 1,378,016 + ABZR87_RS22365 0.78 +0.1 1,378,016 + ABZR87_RS22365 0.78 +0.1 1,378,017 - ABZR87_RS22365 0.78 -0.9 1,378,017 - ABZR87_RS22365 0.78 +0.5 1,378,017 - ABZR87_RS22365 0.78 -2.0 1,378,017 - ABZR87_RS22365 0.78 -0.1 1,378,180 + ABZR87_RS22365 0.87 -0.9 1,378,180 + ABZR87_RS22365 0.87 +0.4 1,378,394 + -0.8 1,378,394 + -1.3 1,378,394 + +0.3 1,378,396 + -0.2 1,378,396 + +0.3 1,378,396 + +0.1 1,378,397 - +1.8 1,378,397 - +0.4 1,378,397 - -0.5 1,378,397 - -0.6 1,378,397 - -0.8 1,378,397 - -0.3 1,378,397 - +0.0 1,378,436 + +1.2 1,378,436 + +0.2 1,378,436 + -0.7 1,378,437 - -1.8 1,378,678 + ABZR87_RS22370 0.39 -0.3 1,378,694 + ABZR87_RS22370 0.43 -0.5 1,378,929 - +0.2 1,378,986 + ABZR87_RS22375 0.12 -0.4 1,378,986 + ABZR87_RS22375 0.12 -0.1 1,378,986 + ABZR87_RS22375 0.12 -1.0 1,378,987 - ABZR87_RS22375 0.12 +0.1 1,378,987 - ABZR87_RS22375 0.12 -0.7 1,378,987 - ABZR87_RS22375 0.12 -0.6 1,379,034 + ABZR87_RS22375 0.22 +0.1 1,379,034 + ABZR87_RS22375 0.22 +0.4 1,379,034 + ABZR87_RS22375 0.22 -0.3 1,379,034 + ABZR87_RS22375 0.22 -1.6 1,379,035 - ABZR87_RS22375 0.22 +2.1 1,379,632 + +0.3 1,379,632 + -0.4 1,379,633 - -0.4 1,379,633 - +0.2 1,379,633 - +0.4 1,379,690 + ABZR87_RS22380 0.12 -0.3 1,379,690 + ABZR87_RS22380 0.12 +0.0 1,379,690 + ABZR87_RS22380 0.12 +0.5 1,379,691 - ABZR87_RS22380 0.12 +1.7 1,379,691 - ABZR87_RS22380 0.12 -0.1 1,379,902 + ABZR87_RS22380 0.25 +0.4 1,379,902 + ABZR87_RS22380 0.25 -0.7 1,379,902 + ABZR87_RS22380 0.25 -0.1 1,379,902 + ABZR87_RS22380 0.25 -0.4 1,379,903 - ABZR87_RS22380 0.25 -0.2
Or see this region's nucleotide sequence