Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21825 and ABZR87_RS21830 overlap by 17 nucleotides ABZR87_RS21830 and ABZR87_RS21835 overlap by 11 nucleotides ABZR87_RS21835 and ABZR87_RS21840 overlap by 4 nucleotides ABZR87_RS21840 and ABZR87_RS21845 are separated by 16 nucleotides
ABZR87_RS21825: ABZR87_RS21825 - metalloregulator ArsR/SmtB family transcription factor, at 1,279,607 to 1,279,963
_RS21825
ABZR87_RS21830: ABZR87_RS21830 - SRPBCC family protein, at 1,279,947 to 1,280,909
_RS21830
ABZR87_RS21835: ABZR87_RS21835 - hypothetical protein, at 1,280,899 to 1,281,024
_RS21835
ABZR87_RS21840: ABZR87_RS21840 - DUF4865 family protein, at 1,281,021 to 1,281,587
_RS21840
ABZR87_RS21845: ABZR87_RS21845 - tautomerase family protein, at 1,281,604 to 1,281,996
_RS21845
Position (kb)
1280
1281
1282 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1279.913 kb on + strand, within ABZR87_RS21825 at 1279.913 kb on + strand, within ABZR87_RS21825 at 1279.914 kb on - strand, within ABZR87_RS21825 at 1279.914 kb on - strand, within ABZR87_RS21825 at 1279.914 kb on - strand, within ABZR87_RS21825 at 1280.572 kb on - strand, within ABZR87_RS21830 at 1280.703 kb on + strand, within ABZR87_RS21830 at 1280.703 kb on + strand, within ABZR87_RS21830 at 1280.703 kb on + strand, within ABZR87_RS21830 at 1280.704 kb on - strand, within ABZR87_RS21830 at 1280.704 kb on - strand, within ABZR87_RS21830 at 1280.709 kb on + strand, within ABZR87_RS21830 at 1280.709 kb on + strand, within ABZR87_RS21830 at 1280.709 kb on + strand, within ABZR87_RS21830 at 1280.709 kb on - strand, within ABZR87_RS21830 at 1280.710 kb on - strand, within ABZR87_RS21830 at 1280.710 kb on - strand, within ABZR87_RS21830 at 1280.710 kb on - strand, within ABZR87_RS21830 at 1280.710 kb on - strand, within ABZR87_RS21830 at 1280.835 kb on + strand at 1280.835 kb on + strand at 1280.996 kb on + strand, within ABZR87_RS21835 at 1280.996 kb on + strand, within ABZR87_RS21835 at 1280.996 kb on + strand, within ABZR87_RS21835 at 1280.996 kb on + strand, within ABZR87_RS21835 at 1280.997 kb on - strand, within ABZR87_RS21835 at 1280.997 kb on - strand, within ABZR87_RS21835 at 1281.409 kb on + strand, within ABZR87_RS21840 at 1281.409 kb on + strand, within ABZR87_RS21840 at 1281.409 kb on + strand, within ABZR87_RS21840 at 1281.410 kb on - strand, within ABZR87_RS21840 at 1281.547 kb on + strand at 1281.548 kb on - strand at 1281.571 kb on + strand at 1281.571 kb on + strand at 1281.571 kb on + strand at 1281.571 kb on + strand at 1281.572 kb on - strand at 1281.572 kb on - strand at 1281.623 kb on + strand at 1281.624 kb on - strand at 1281.671 kb on - strand, within ABZR87_RS21845 at 1281.671 kb on - strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.685 kb on + strand, within ABZR87_RS21845 at 1281.686 kb on - strand, within ABZR87_RS21845 at 1281.686 kb on - strand, within ABZR87_RS21845 at 1281.827 kb on + strand, within ABZR87_RS21845 at 1281.827 kb on + strand, within ABZR87_RS21845 at 1281.827 kb on + strand, within ABZR87_RS21845 at 1281.828 kb on - strand, within ABZR87_RS21845 at 1281.923 kb on + strand, within ABZR87_RS21845 at 1281.950 kb on + strand, within ABZR87_RS21845 at 1281.951 kb on - strand, within ABZR87_RS21845
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,279,913 + ABZR87_RS21825 0.86 -0.0 1,279,913 + ABZR87_RS21825 0.86 -0.0 1,279,914 - ABZR87_RS21825 0.86 +0.0 1,279,914 - ABZR87_RS21825 0.86 +0.5 1,279,914 - ABZR87_RS21825 0.86 -0.4 1,280,572 - ABZR87_RS21830 0.65 +2.1 1,280,703 + ABZR87_RS21830 0.79 +0.0 1,280,703 + ABZR87_RS21830 0.79 +0.8 1,280,703 + ABZR87_RS21830 0.79 +0.9 1,280,704 - ABZR87_RS21830 0.79 -0.7 1,280,704 - ABZR87_RS21830 0.79 +0.2 1,280,709 + ABZR87_RS21830 0.79 -0.4 1,280,709 + ABZR87_RS21830 0.79 +0.1 1,280,709 + ABZR87_RS21830 0.79 +0.1 1,280,709 - ABZR87_RS21830 0.79 +0.3 1,280,710 - ABZR87_RS21830 0.79 +0.9 1,280,710 - ABZR87_RS21830 0.79 +0.0 1,280,710 - ABZR87_RS21830 0.79 +0.2 1,280,710 - ABZR87_RS21830 0.79 -0.1 1,280,835 + +0.5 1,280,835 + +0.1 1,280,996 + ABZR87_RS21835 0.77 +0.4 1,280,996 + ABZR87_RS21835 0.77 +1.9 1,280,996 + ABZR87_RS21835 0.77 +0.3 1,280,996 + ABZR87_RS21835 0.77 -1.0 1,280,997 - ABZR87_RS21835 0.78 +0.8 1,280,997 - ABZR87_RS21835 0.78 +1.2 1,281,409 + ABZR87_RS21840 0.68 -0.4 1,281,409 + ABZR87_RS21840 0.68 +0.3 1,281,409 + ABZR87_RS21840 0.68 +0.1 1,281,410 - ABZR87_RS21840 0.69 -0.1 1,281,547 + -0.5 1,281,548 - +0.7 1,281,571 + +0.4 1,281,571 + +1.2 1,281,571 + -0.2 1,281,571 + +1.2 1,281,572 - +1.7 1,281,572 - -0.5 1,281,623 + -0.0 1,281,624 - -0.9 1,281,671 - ABZR87_RS21845 0.17 +0.3 1,281,671 - ABZR87_RS21845 0.17 +0.4 1,281,685 + ABZR87_RS21845 0.21 -0.9 1,281,685 + ABZR87_RS21845 0.21 -0.2 1,281,685 + ABZR87_RS21845 0.21 +1.0 1,281,685 + ABZR87_RS21845 0.21 -0.0 1,281,685 + ABZR87_RS21845 0.21 +0.5 1,281,685 + ABZR87_RS21845 0.21 -2.2 1,281,685 + ABZR87_RS21845 0.21 -0.2 1,281,685 + ABZR87_RS21845 0.21 +0.2 1,281,685 + ABZR87_RS21845 0.21 -0.1 1,281,686 - ABZR87_RS21845 0.21 -0.2 1,281,686 - ABZR87_RS21845 0.21 +0.5 1,281,827 + ABZR87_RS21845 0.57 -0.2 1,281,827 + ABZR87_RS21845 0.57 -1.3 1,281,827 + ABZR87_RS21845 0.57 +0.7 1,281,828 - ABZR87_RS21845 0.57 +0.8 1,281,923 + ABZR87_RS21845 0.81 +0.3 1,281,950 + ABZR87_RS21845 0.88 -0.2 1,281,951 - ABZR87_RS21845 0.88 +0.1
Or see this region's nucleotide sequence