Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21720 and ABZR87_RS21725 are separated by 196 nucleotides ABZR87_RS21725 and ABZR87_RS21730 overlap by 1 nucleotides ABZR87_RS21730 and ABZR87_RS21735 are separated by 9 nucleotides ABZR87_RS21735 and ABZR87_RS21740 are separated by 252 nucleotides
ABZR87_RS21720: ABZR87_RS21720 - hypothetical protein, at 1,258,560 to 1,258,913
_RS21720
ABZR87_RS21725: ABZR87_RS21725 - heavy metal response regulator transcription factor, at 1,259,110 to 1,259,784
_RS21725
ABZR87_RS21730: ABZR87_RS21730 - heavy metal sensor histidine kinase, at 1,259,784 to 1,261,226
_RS21730
ABZR87_RS21735: ABZR87_RS21735 - DUF2917 domain-containing protein, at 1,261,236 to 1,261,598
_RS21735
ABZR87_RS21740: ABZR87_RS21740 - TauD/TfdA family dioxygenase, at 1,261,851 to 1,262,807
_RS21740
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1259.064 kb on + strand at 1259.064 kb on + strand at 1259.065 kb on - strand at 1259.065 kb on - strand at 1259.153 kb on - strand at 1259.242 kb on + strand, within ABZR87_RS21725 at 1259.243 kb on - strand, within ABZR87_RS21725 at 1259.243 kb on - strand, within ABZR87_RS21725 at 1259.243 kb on - strand, within ABZR87_RS21725 at 1259.243 kb on - strand, within ABZR87_RS21725 at 1259.243 kb on - strand, within ABZR87_RS21725 at 1259.398 kb on + strand, within ABZR87_RS21725 at 1259.398 kb on + strand, within ABZR87_RS21725 at 1259.398 kb on + strand, within ABZR87_RS21725 at 1259.399 kb on - strand, within ABZR87_RS21725 at 1259.569 kb on + strand, within ABZR87_RS21725 at 1259.570 kb on - strand, within ABZR87_RS21725 at 1259.639 kb on + strand, within ABZR87_RS21725 at 1259.892 kb on + strand at 1259.892 kb on + strand at 1259.892 kb on + strand at 1259.893 kb on - strand at 1259.893 kb on - strand at 1259.893 kb on - strand at 1259.898 kb on + strand at 1259.898 kb on + strand at 1259.899 kb on - strand at 1259.987 kb on - strand, within ABZR87_RS21730 at 1260.024 kb on + strand, within ABZR87_RS21730 at 1260.024 kb on + strand, within ABZR87_RS21730 at 1260.027 kb on - strand, within ABZR87_RS21730 at 1260.172 kb on + strand, within ABZR87_RS21730 at 1260.306 kb on + strand, within ABZR87_RS21730 at 1260.306 kb on + strand, within ABZR87_RS21730 at 1260.307 kb on - strand, within ABZR87_RS21730 at 1260.648 kb on + strand, within ABZR87_RS21730 at 1260.649 kb on - strand, within ABZR87_RS21730 at 1260.681 kb on + strand, within ABZR87_RS21730 at 1260.681 kb on + strand, within ABZR87_RS21730 at 1260.682 kb on - strand, within ABZR87_RS21730 at 1260.801 kb on + strand, within ABZR87_RS21730 at 1261.266 kb on - strand at 1261.331 kb on + strand, within ABZR87_RS21735 at 1261.778 kb on - strand at 1261.816 kb on + strand at 1261.816 kb on + strand at 1261.816 kb on + strand at 1261.817 kb on - strand at 1261.903 kb on + strand at 1261.922 kb on + strand at 1261.923 kb on - strand at 1261.989 kb on + strand, within ABZR87_RS21740 at 1262.007 kb on + strand, within ABZR87_RS21740 at 1262.007 kb on + strand, within ABZR87_RS21740 at 1262.007 kb on + strand, within ABZR87_RS21740 at 1262.008 kb on - strand, within ABZR87_RS21740 at 1262.008 kb on - strand, within ABZR87_RS21740 at 1262.008 kb on - strand, within ABZR87_RS21740 at 1262.008 kb on - strand, within ABZR87_RS21740
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,259,064 + +0.2 1,259,064 + -0.3 1,259,065 - -0.8 1,259,065 - +0.5 1,259,153 - +0.1 1,259,242 + ABZR87_RS21725 0.20 -1.2 1,259,243 - ABZR87_RS21725 0.20 -0.8 1,259,243 - ABZR87_RS21725 0.20 -2.9 1,259,243 - ABZR87_RS21725 0.20 -2.3 1,259,243 - ABZR87_RS21725 0.20 -0.8 1,259,243 - ABZR87_RS21725 0.20 +0.6 1,259,398 + ABZR87_RS21725 0.43 -0.5 1,259,398 + ABZR87_RS21725 0.43 -0.6 1,259,398 + ABZR87_RS21725 0.43 -2.2 1,259,399 - ABZR87_RS21725 0.43 -0.5 1,259,569 + ABZR87_RS21725 0.68 +0.0 1,259,570 - ABZR87_RS21725 0.68 -0.3 1,259,639 + ABZR87_RS21725 0.78 -1.1 1,259,892 + -0.3 1,259,892 + -0.8 1,259,892 + +1.3 1,259,893 - -0.3 1,259,893 - +0.3 1,259,893 - +0.2 1,259,898 + -0.9 1,259,898 + +0.9 1,259,899 - -0.3 1,259,987 - ABZR87_RS21730 0.14 +0.8 1,260,024 + ABZR87_RS21730 0.17 -1.1 1,260,024 + ABZR87_RS21730 0.17 -1.5 1,260,027 - ABZR87_RS21730 0.17 +0.3 1,260,172 + ABZR87_RS21730 0.27 +1.4 1,260,306 + ABZR87_RS21730 0.36 +0.6 1,260,306 + ABZR87_RS21730 0.36 -0.5 1,260,307 - ABZR87_RS21730 0.36 +0.1 1,260,648 + ABZR87_RS21730 0.60 -0.4 1,260,649 - ABZR87_RS21730 0.60 -0.5 1,260,681 + ABZR87_RS21730 0.62 +1.6 1,260,681 + ABZR87_RS21730 0.62 -0.1 1,260,682 - ABZR87_RS21730 0.62 +0.1 1,260,801 + ABZR87_RS21730 0.70 +0.2 1,261,266 - -0.0 1,261,331 + ABZR87_RS21735 0.26 +0.4 1,261,778 - +0.3 1,261,816 + +0.2 1,261,816 + +0.8 1,261,816 + -0.1 1,261,817 - -0.1 1,261,903 + +0.2 1,261,922 + +0.8 1,261,923 - +0.0 1,261,989 + ABZR87_RS21740 0.14 -0.2 1,262,007 + ABZR87_RS21740 0.16 +0.4 1,262,007 + ABZR87_RS21740 0.16 -0.5 1,262,007 + ABZR87_RS21740 0.16 +1.5 1,262,008 - ABZR87_RS21740 0.16 +0.3 1,262,008 - ABZR87_RS21740 0.16 +0.3 1,262,008 - ABZR87_RS21740 0.16 -0.9 1,262,008 - ABZR87_RS21740 0.16 -0.2
Or see this region's nucleotide sequence