Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21695 and ABZR87_RS21700 are separated by 37 nucleotides ABZR87_RS21700 and ABZR87_RS21705 are separated by 35 nucleotides ABZR87_RS21705 and ABZR87_RS21710 are separated by 11 nucleotides
ABZR87_RS21695: ABZR87_RS21695 - PadR family transcriptional regulator, at 1,252,010 to 1,252,639
_RS21695
ABZR87_RS21700: ABZR87_RS21700 - hypothetical protein, at 1,252,677 to 1,252,856
_RS21700
ABZR87_RS21705: ABZR87_RS21705 - CusA/CzcA family heavy metal efflux RND transporter, at 1,252,892 to 1,256,062
_RS21705
ABZR87_RS21710: ABZR87_RS21710 - efflux RND transporter periplasmic adaptor subunit, at 1,256,074 to 1,257,264
_RS21710
Position (kb)
1252
1253
1254
1255
1256
1257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1252.080 kb on - strand, within ABZR87_RS21695 at 1252.136 kb on + strand, within ABZR87_RS21695 at 1252.148 kb on + strand, within ABZR87_RS21695 at 1252.148 kb on + strand, within ABZR87_RS21695 at 1252.149 kb on - strand, within ABZR87_RS21695 at 1252.235 kb on + strand, within ABZR87_RS21695 at 1252.310 kb on + strand, within ABZR87_RS21695 at 1252.311 kb on - strand, within ABZR87_RS21695 at 1252.311 kb on - strand, within ABZR87_RS21695 at 1252.311 kb on - strand, within ABZR87_RS21695 at 1252.311 kb on - strand, within ABZR87_RS21695 at 1252.311 kb on - strand, within ABZR87_RS21695 at 1252.333 kb on - strand, within ABZR87_RS21695 at 1252.764 kb on + strand, within ABZR87_RS21700 at 1252.765 kb on - strand, within ABZR87_RS21700 at 1252.954 kb on - strand at 1252.966 kb on - strand at 1253.150 kb on + strand at 1253.150 kb on + strand at 1253.278 kb on + strand, within ABZR87_RS21705 at 1253.832 kb on + strand, within ABZR87_RS21705 at 1253.833 kb on - strand, within ABZR87_RS21705 at 1253.833 kb on - strand, within ABZR87_RS21705 at 1253.941 kb on + strand, within ABZR87_RS21705 at 1254.009 kb on - strand, within ABZR87_RS21705 at 1254.060 kb on + strand, within ABZR87_RS21705 at 1254.177 kb on + strand, within ABZR87_RS21705 at 1254.177 kb on + strand, within ABZR87_RS21705 at 1254.178 kb on - strand, within ABZR87_RS21705 at 1254.178 kb on - strand, within ABZR87_RS21705 at 1254.178 kb on - strand, within ABZR87_RS21705 at 1254.178 kb on - strand, within ABZR87_RS21705 at 1254.178 kb on - strand, within ABZR87_RS21705 at 1254.486 kb on + strand, within ABZR87_RS21705 at 1254.486 kb on + strand, within ABZR87_RS21705 at 1254.486 kb on + strand, within ABZR87_RS21705 at 1254.486 kb on + strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.487 kb on - strand, within ABZR87_RS21705 at 1254.676 kb on - strand, within ABZR87_RS21705 at 1254.702 kb on + strand, within ABZR87_RS21705 at 1254.702 kb on + strand, within ABZR87_RS21705 at 1254.703 kb on - strand, within ABZR87_RS21705 at 1255.074 kb on + strand, within ABZR87_RS21705 at 1255.074 kb on + strand, within ABZR87_RS21705 at 1255.075 kb on - strand, within ABZR87_RS21705 at 1255.357 kb on - strand, within ABZR87_RS21705 at 1255.357 kb on - strand, within ABZR87_RS21705 at 1255.357 kb on - strand, within ABZR87_RS21705 at 1255.357 kb on - strand, within ABZR87_RS21705 at 1255.357 kb on - strand, within ABZR87_RS21705 at 1255.380 kb on + strand, within ABZR87_RS21705 at 1255.381 kb on - strand, within ABZR87_RS21705 at 1255.446 kb on + strand, within ABZR87_RS21705 at 1255.446 kb on + strand, within ABZR87_RS21705 at 1255.447 kb on - strand, within ABZR87_RS21705 at 1255.447 kb on - strand, within ABZR87_RS21705 at 1255.590 kb on + strand, within ABZR87_RS21705 at 1255.591 kb on - strand, within ABZR87_RS21705 at 1255.631 kb on + strand, within ABZR87_RS21705 at 1255.644 kb on + strand, within ABZR87_RS21705 at 1255.644 kb on + strand, within ABZR87_RS21705 at 1255.752 kb on + strand at 1255.863 kb on + strand at 1255.863 kb on + strand at 1255.863 kb on + strand at 1255.863 kb on + strand at 1255.863 kb on + strand at 1255.864 kb on - strand at 1255.864 kb on - strand at 1255.912 kb on + strand at 1255.969 kb on + strand at 1255.970 kb on - strand at 1255.970 kb on - strand at 1255.970 kb on - strand at 1255.970 kb on - strand at 1255.971 kb on + strand at 1255.971 kb on + strand at 1255.971 kb on + strand at 1255.972 kb on - strand at 1255.972 kb on - strand at 1255.972 kb on - strand at 1255.972 kb on - strand at 1255.977 kb on + strand at 1255.977 kb on + strand at 1255.978 kb on - strand at 1256.120 kb on + strand at 1256.120 kb on + strand at 1256.121 kb on - strand at 1256.121 kb on - strand at 1256.121 kb on - strand at 1256.121 kb on - strand at 1256.121 kb on - strand at 1256.138 kb on + strand at 1256.423 kb on + strand, within ABZR87_RS21710 at 1256.423 kb on + strand, within ABZR87_RS21710 at 1256.423 kb on + strand, within ABZR87_RS21710 at 1256.423 kb on + strand, within ABZR87_RS21710 at 1256.424 kb on - strand, within ABZR87_RS21710 at 1256.424 kb on - strand, within ABZR87_RS21710 at 1256.424 kb on - strand, within ABZR87_RS21710 at 1256.424 kb on - strand, within ABZR87_RS21710 at 1256.751 kb on - strand, within ABZR87_RS21710
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,252,080 - ABZR87_RS21695 0.11 -0.2 1,252,136 + ABZR87_RS21695 0.20 -1.0 1,252,148 + ABZR87_RS21695 0.22 -1.1 1,252,148 + ABZR87_RS21695 0.22 -1.6 1,252,149 - ABZR87_RS21695 0.22 +0.0 1,252,235 + ABZR87_RS21695 0.36 -0.2 1,252,310 + ABZR87_RS21695 0.48 +0.8 1,252,311 - ABZR87_RS21695 0.48 -0.0 1,252,311 - ABZR87_RS21695 0.48 +0.2 1,252,311 - ABZR87_RS21695 0.48 +0.4 1,252,311 - ABZR87_RS21695 0.48 +0.4 1,252,311 - ABZR87_RS21695 0.48 -0.0 1,252,333 - ABZR87_RS21695 0.51 -0.4 1,252,764 + ABZR87_RS21700 0.48 -0.5 1,252,765 - ABZR87_RS21700 0.49 +0.4 1,252,954 - -0.3 1,252,966 - -1.1 1,253,150 + -1.9 1,253,150 + +1.3 1,253,278 + ABZR87_RS21705 0.12 -0.8 1,253,832 + ABZR87_RS21705 0.30 -1.0 1,253,833 - ABZR87_RS21705 0.30 -1.5 1,253,833 - ABZR87_RS21705 0.30 +0.3 1,253,941 + ABZR87_RS21705 0.33 +0.9 1,254,009 - ABZR87_RS21705 0.35 -1.0 1,254,060 + ABZR87_RS21705 0.37 -0.3 1,254,177 + ABZR87_RS21705 0.41 +0.3 1,254,177 + ABZR87_RS21705 0.41 -0.9 1,254,178 - ABZR87_RS21705 0.41 -0.0 1,254,178 - ABZR87_RS21705 0.41 -1.3 1,254,178 - ABZR87_RS21705 0.41 +0.3 1,254,178 - ABZR87_RS21705 0.41 +2.1 1,254,178 - ABZR87_RS21705 0.41 +0.6 1,254,486 + ABZR87_RS21705 0.50 +0.5 1,254,486 + ABZR87_RS21705 0.50 +0.4 1,254,486 + ABZR87_RS21705 0.50 -0.5 1,254,486 + ABZR87_RS21705 0.50 +0.1 1,254,487 - ABZR87_RS21705 0.50 +0.3 1,254,487 - ABZR87_RS21705 0.50 -1.0 1,254,487 - ABZR87_RS21705 0.50 -1.8 1,254,487 - ABZR87_RS21705 0.50 +0.3 1,254,487 - ABZR87_RS21705 0.50 +0.7 1,254,487 - ABZR87_RS21705 0.50 +1.5 1,254,676 - ABZR87_RS21705 0.56 -0.0 1,254,702 + ABZR87_RS21705 0.57 +0.9 1,254,702 + ABZR87_RS21705 0.57 -0.3 1,254,703 - ABZR87_RS21705 0.57 -1.2 1,255,074 + ABZR87_RS21705 0.69 -0.9 1,255,074 + ABZR87_RS21705 0.69 -0.5 1,255,075 - ABZR87_RS21705 0.69 -0.0 1,255,357 - ABZR87_RS21705 0.78 +0.2 1,255,357 - ABZR87_RS21705 0.78 -0.6 1,255,357 - ABZR87_RS21705 0.78 +2.1 1,255,357 - ABZR87_RS21705 0.78 -0.8 1,255,357 - ABZR87_RS21705 0.78 -0.7 1,255,380 + ABZR87_RS21705 0.78 +1.7 1,255,381 - ABZR87_RS21705 0.78 -0.1 1,255,446 + ABZR87_RS21705 0.81 +1.2 1,255,446 + ABZR87_RS21705 0.81 -2.0 1,255,447 - ABZR87_RS21705 0.81 +0.2 1,255,447 - ABZR87_RS21705 0.81 -0.1 1,255,590 + ABZR87_RS21705 0.85 -0.3 1,255,591 - ABZR87_RS21705 0.85 -0.4 1,255,631 + ABZR87_RS21705 0.86 +0.1 1,255,644 + ABZR87_RS21705 0.87 -0.4 1,255,644 + ABZR87_RS21705 0.87 -0.1 1,255,752 + -3.5 1,255,863 + +2.2 1,255,863 + +1.7 1,255,863 + -2.7 1,255,863 + +0.2 1,255,863 + +0.1 1,255,864 - +1.2 1,255,864 - +0.6 1,255,912 + +1.4 1,255,969 + -0.4 1,255,970 - +0.0 1,255,970 - -0.5 1,255,970 - -0.1 1,255,970 - -0.6 1,255,971 + -0.8 1,255,971 + -2.3 1,255,971 + +1.1 1,255,972 - -0.2 1,255,972 - -0.2 1,255,972 - +0.1 1,255,972 - +1.6 1,255,977 + +0.9 1,255,977 + -0.9 1,255,978 - -1.5 1,256,120 + +0.8 1,256,120 + -0.3 1,256,121 - +0.6 1,256,121 - +0.1 1,256,121 - +1.6 1,256,121 - +2.7 1,256,121 - -1.3 1,256,138 + -0.2 1,256,423 + ABZR87_RS21710 0.29 -1.2 1,256,423 + ABZR87_RS21710 0.29 -1.5 1,256,423 + ABZR87_RS21710 0.29 +0.2 1,256,423 + ABZR87_RS21710 0.29 +0.2 1,256,424 - ABZR87_RS21710 0.29 +0.4 1,256,424 - ABZR87_RS21710 0.29 +0.3 1,256,424 - ABZR87_RS21710 0.29 +1.6 1,256,424 - ABZR87_RS21710 0.29 +1.5 1,256,751 - ABZR87_RS21710 0.57 +0.8
Or see this region's nucleotide sequence